公开(公告)号：US20150197760A1

公开(公告)日：2015-07-16

申请号：US14408089

申请日：2013-06-17

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter Los ,  Wilbert Herman Marie Heijne ,  Herman Jan Pel ,  Robbertus Antonius Damveld ,  Brenda Vonk

IPC: C12N15/80 ,  C12N9/34

CPC classification number: C12N15/80 ,  C12N9/18 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2434 ,  C12P1/02 ,  C12P21/02

Abstract: The present invention relates to isolated Rasamsonia promoter DNA sequences, to DNA constructs, vectors, and host cells comprising these promoters in operative association with coding sequences. The present invention also relates to methods for expressing a gene and/or producing a biological compound using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.

Abstract translation: 本发明涉及分离的Rasamsonia启动子DNA序列，涉及包含这些启动子的DNA构建体，载体和宿主细胞，其与编码序列有效结合。 本发明还涉及使用分离的新启动子来表达基因和/或生产生物化合物的方法。 本发明还涉及使用本发明的新启动子改变内源基因的转录水平和/或调节的方法。

公开(公告)号：US11149268B2

公开(公告)日：2021-10-19

申请号：US16319746

申请日：2016-12-15

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries Roubos ,  René Verwaal ,  Bianca Elisabeth Maria Gielesen ,  Brenda Vonk

IPC: C12N15/09 ,  C12N15/10 ,  C12N15/11 ,  C12N15/90

Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct.
The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.

公开(公告)号：US11118193B2

公开(公告)日：2021-09-14

申请号：US16780063

申请日：2020-02-03

Applicant: DSM IP ASSETS B.V.

Inventor： René Verwaal ,  Bernard Meijrink ,  Nathalie Wiessenhaan ,  Brenda Vonk ,  Johannes Andries Roubos

IPC: C12N15/90 ,  C12P19/34 ,  C12N15/64 ,  C07H21/02 ,  C12N5/00 ,  C12N15/82 ,  C12N15/113 ,  C12N9/22 ,  C12N15/81 ,  C12N15/10 ,  C12P23/00

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.