公开(公告)号：US20150197760A1

公开(公告)日：2015-07-16

申请号：US14408089

申请日：2013-06-17

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter Los ,  Wilbert Herman Marie Heijne ,  Herman Jan Pel ,  Robbertus Antonius Damveld ,  Brenda Vonk

IPC: C12N15/80 ,  C12N9/34

CPC classification number: C12N15/80 ,  C12N9/18 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2434 ,  C12P1/02 ,  C12P21/02

Abstract: The present invention relates to isolated Rasamsonia promoter DNA sequences, to DNA constructs, vectors, and host cells comprising these promoters in operative association with coding sequences. The present invention also relates to methods for expressing a gene and/or producing a biological compound using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.

Abstract translation: 本发明涉及分离的Rasamsonia启动子DNA序列，涉及包含这些启动子的DNA构建体，载体和宿主细胞，其与编码序列有效结合。 本发明还涉及使用分离的新启动子来表达基因和/或生产生物化合物的方法。 本发明还涉及使用本发明的新启动子改变内源基因的转录水平和/或调节的方法。

公开(公告)号：US09499841B2

公开(公告)日：2016-11-22

申请号：US14750084

申请日：2015-06-25

Applicant: DSM IP ASSETS B.V.

Inventor： Paul Klaassen ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom ,  Wilbert Herman Marie Heijne

IPC: C07K14/395 ,  C12P7/10 ,  C12N1/36 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/24 ,  C12N9/26 ,  C12N9/42 ,  C12N9/38 ,  C12N9/50 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P13/00 ,  C12P13/08 ,  C12P13/12 ,  C12P13/20 ,  C12P13/22 ,  C12P17/10 ,  C12P35/00

CPC classification number: C12P7/10 ,  C07K14/395 ,  C12N1/36 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/10 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/2405 ,  C12N9/2411 ,  C12N9/2437 ,  C12N9/2468 ,  C12N9/248 ,  C12N9/50 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12P5/026 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P13/005 ,  C12P13/08 ,  C12P13/12 ,  C12P13/20 ,  C12P13/227 ,  C12P17/10 ,  C12P35/00 ,  C12Y101/01 ,  C12Y207/01016 ,  C12Y501/03004 ,  C12Y503/01004 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

Abstract: The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.