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公开(公告)号：US10294479B2
公开(公告)日：2019-05-21
申请号：US15307455
申请日：2015-03-04
Inventor： Jianhe Xu , Yujun Zhang , Gaowei Zheng , Jiang Pan
IPC: C12N9/04 , C12N9/88 , C12N9/10 , C12N15/00 , C12P7/62 , C12P7/04 , C12N15/52 , C12R1/72 , C12N1/16 , C12P7/42 , C12N9/02 , C12N15/81
Abstract: Disclosed herein is Candida parapsilosis CGMCC 9630, the carbonyl reductase expressed by said strain and the encoding gene and amino acid sequence thereof, the recombinant expression vector and recombinant expression transformant containing said gene sequence, and use of whole cells of Candida parapsilosis, carbonyl reductase or corresponding recombinant transformant thereof as catalyst in catalyzing asymmetric reduction of prochiral carbonyl compounds, particularly reduction of 6-carbonyl-8-halogenocaprylate to prepare the synthetic precursor of (R)-α-lipoic acid, (R)-6-hydroxy-8-halogenocaprylate. In comparison to other methods of asymmetric reduction for preparing (R)-6-hydroxy-8-halogenocaprylate, the disclosure has advantages of high substrate concentration, mild reaction conditions, environmental friendship, high yield, and high optical purity of the product, and thus has good prospect in industrial production of (R)-α-α-lipoic acid.
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公开(公告)号：US11001866B2
公开(公告)日：2021-05-11
申请号：US16620529
申请日：2017-10-25
Applicant: JIANGSU AOSAIKANG PHARMACEUTICAL CO., LTD. , EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Abstract: A strain of Burkholderia is Burkholderia glathei ECU0712, with an accession number of CGMCC NO. 14464. With the strain or its extract as the biocatalyst, thioether is catalyzed to be oxidized asymmetrically to chiral sulfoxide, with significant advantages that the obtained product has a high optical purity, and benefits of a simple reaction system, short preparation time of the catalyst and a high yield of the product.
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公开(公告)号：US10787651B2
公开(公告)日：2020-09-29
申请号：US16620525
申请日：2018-04-13
Applicant: JIANGSU AOSAIKANG PHARMACEUTICAL CO., LTD. , EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Abstract: A Bradyrhizobium monooxygenase, a gene for encoding the monooxygenase, a recombinant expression vector comprising the gene and a recombinant transformant, a method of preparing the monooxygenase by the recombinant expression transformant, and a method of preparing an optically pure chiral sulfoxide by the monooxygenase, in particular to a method of preparing prazole drugs by means of catalyzing the asymmetric oxidation of thioether, a prazole precursor. As compared with other methods of preparing an optically pure sulfoxide, the product produced by the monooxygenase of the present invention as a catalyst has high optical purity, avoids the generation of the byproduct sulfone, and has advantages of mild reaction conditions, simple and convenient operations, easy amplification, etc.
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公开(公告)号：US20180265875A1
公开(公告)日：2018-09-20
申请号：US15307455
申请日：2015-03-04
Inventor： Jianhe Xu , Yujun Zhang , Gaowei Zheng , Jiang Pan
CPC classification number: C12N15/52 , C12N1/16 , C12N9/0036 , C12N15/81 , C12P7/42 , C12R1/72 , C12Y101/01184
Abstract: Disclosed herein is Candida parapsilosis CGMCC 9630, the carbonyl reductase expressed by said strain and the encoding gene and amino acid sequence thereof, the recombinant expression vector and recombinant expression transformant containing said gene sequence, and use of whole cells of Candida parapsilosis, carbonyl reductase or corresponding recombinant transformant thereof as catalyst in catalyzing asymmetric reduction of prochiral carbonyl compounds, particularly reduction of 6-carbonyl-8-halogenocaprylate to prepare the synthetic precursor of (R)-α-lipoic acid, (R)-6-hydroxy-8-halogenocaprylate. In comparison to other methods of asymmetric reduction for preparing (R)-6-hydroxy-8-halogenocaprylate, the disclosure has advantages of high substrate concentration, mild reaction conditions, environmental friendship, high yield, and high optical purity of the product, and thus has good prospect in industrial production of (R)-α-α-lipoic acid.
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