公开(公告)号：US20050267062A1

公开(公告)日：2005-12-01

申请号：US11115922

申请日：2005-04-26

申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen Eisenberg ,  Eric Jarvis

发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen Eisenberg ,  Eric Jarvis

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K48/00 ,  A61P9/00 ,  A61P9/10 ,  A61P17/02 ,  A61P19/02 ,  A61P29/00 ,  A61P35/00 ,  A61P43/00 ,  C07K14/47 ,  C07K14/52 ,  C12N15/12 ,  C12N15/861 ,  C12N15/867 ,  C12Q1/02

CPC分类号： A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。

公开(公告)号：US20070072818A1

公开(公告)日：2007-03-29

申请号：US11486994

申请日：2006-07-14

申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen Eisenberg ,  Eric Jarvis

发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen Eisenberg ,  Eric Jarvis

IPC分类号： A61K48/00

CPC分类号： A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。

公开(公告)号：US07067317B2

公开(公告)日：2006-06-27

申请号：US09846033

申请日：2001-04-30

申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

IPC分类号： C12Q1/68 ,  C12N5/16 ,  C12N15/64

CPC分类号： C07K14/52 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K2319/00 ,  C12N2799/021

摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

公开(公告)号：US07605140B2

公开(公告)日：2009-10-20

申请号：US11115922

申请日：2005-04-26

申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

IPC分类号： A61K48/00

CPC分类号： A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

公开(公告)号：US07560440B2

公开(公告)日：2009-07-14

申请号：US11486994

申请日：2006-07-14

申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

IPC分类号： A61K48/00

CPC分类号： A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。

公开(公告)号：US08071564B2

公开(公告)日：2011-12-06

申请号：US12553826

申请日：2009-09-03

申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

IPC分类号： A61K48/00 ,  C07H21/02

CPC分类号： A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。

公开(公告)号：US20100093838A1

公开(公告)日：2010-04-15

申请号：US12553826

申请日：2009-09-03

申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

IPC分类号： A61K31/7088 ,  A61P9/00

CPC分类号： A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。

公开(公告)号：US08153399B2

公开(公告)日：2012-04-10

申请号：US12589884

申请日：2009-10-29

申请人： Pei-Qi Liu ,  Jeffrey C. Miller

发明人： Pei-Qi Liu ,  Jeffrey C. Miller

IPC分类号： C12P21/06 ,  C12N1/20 ,  C12Q1/68 ,  C12Q1/48

CPC分类号： C12N15/85 ,  C07K14/00 ,  C12N9/22 ,  C12N9/93 ,  C12N2510/00 ,  C12P21/02 ,  C12Y603/01002 ,  Y02P20/52

摘要： Disclosed herein are methods and compositions for inactivating a glutamine synthetase (GS) gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.

摘要翻译： 本文公开了使用包含锌指蛋白和切割结构域或裂解半结构域的融合蛋白来灭活谷氨酰胺合成酶（GS）基因的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。

公开(公告)号：US20100311124A1

公开(公告)日：2010-12-09

申请号：US12803928

申请日：2010-07-09

申请人： Pei-Qi Liu ,  Jeffrey C. Miller

发明人： Pei-Qi Liu ,  Jeffrey C. Miller

IPC分类号： C12P21/08 ,  C12N9/99 ,  C12P21/00 ,  C12N5/10

CPC分类号： C12N15/85 ,  C07K14/00 ,  C12N9/22 ,  C12N9/93 ,  C12N2510/00 ,  C12P21/02 ,  C12Y603/01002 ,  Y02P20/52

摘要： Disclosed herein are methods and compositions for inactivating a glutamine synthetase (GS) gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.

公开(公告)号：US20130164785A1

公开(公告)日：2013-06-27

申请号：US13605820

申请日：2012-09-06

申请人： Pei-Qi Liu ,  Jeffrey C. Miller

发明人： Pei-Qi Liu ,  Jeffrey C. Miller

IPC分类号： C07K14/00

CPC分类号： C12N15/85 ,  C07K14/00 ,  C12N9/22 ,  C12N9/93 ,  C12N2510/00 ,  C12P21/02 ,  C12Y603/01002 ,  Y02P20/52

摘要： Disclosed herein are methods and compositions for inactivating a glutamine synthetase (GS) gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.