-
公开(公告)号：US20250011706A1
公开(公告)日：2025-01-09
申请号：US18894014
申请日：2024-09-24
Applicant: FUJIFILM CORPORATION
Inventor： Shinichi NAKAI , Hiroshi SAKUYAMA , Naoto TAKAHASHI
Abstract: A method including: connecting, through a coupling line, a culture section where a culture step, in which cells are cultured in a culture liquid stored in a culture tank and the cells are removed from the culture liquid to obtain a culture supernatant liquid containing a product of the cells, is performed and a purification section where a purification step, in which the product is purified from the culture supernatant liquid to obtain an active pharmaceutical ingredient of a biopharmaceutical, is performed, to allow the culture supernatant liquid to flow into the purification section from the culture section through the coupling line; connecting a flexible single-use tank having an accommodation capacity of 0.2 times to 10 times an amount of the culture liquid in the culture tank to a branch line provided between the culture section and the purification section; and temporarily storing the culture supernatant liquid in the tank.
-
公开(公告)号：US20210355430A1
公开(公告)日：2021-11-18
申请号：US17387091
申请日：2021-07-28
Applicant: FUJIFILM Corporation
Inventor： Naoto TAKAHASHI , Shinichi NAKAI , Hiroshi SAKUYAMA
Abstract: In the cell culture device, a volume of a space defined by a diaphragm of the diaphragm pump and a plane at an outer edge of the diaphragm is 1 cm3 or more and 20 cm3 or less, in a case where an angle formed by a straight line connecting a foot of a perpendicular line from the apex to the plane to each point in the diaphragm and by the perpendicular line is denoted by an angle A, a thickness H of the diaphragm is within a range of 0.5 mm or more and 1.5 mm or less where the angle A is from 0° to 75°, and in a case where a thickness of the diaphragm at the apex is denoted by HT, the thickness H satisfies a relationship of 1≤HT/H≤1.75 at any point where the angle A is from 0° to 75°.
-
公开(公告)号：US20240336671A1
公开(公告)日：2024-10-10
申请号：US18743257
申请日：2024-06-14
Applicant: FUJIFILM Corporation
Inventor： Hiroshi SAKUYAMA , Naoto TAKAHASHI
Abstract: An object of the present invention is to provide a method for producing a product by cell culture, which makes it possible to suppress the clogging of an exhaust filter due to bubbles generated on an upper surface of a culture solution, and a cell culture device that makes it possible to suppress the clogging of an exhaust filter due to bubbles generated on an upper surface of a culture solution. According to the present invention, there is provided a method for producing a product, which includes culturing a cell using a cell culture device to produce a product from the cell, where the cell culture device includes a culture container accommodating a cell suspension containing cells and at least one or more bubble trap containers capable of accumulating bubbles, the one or more bubble trap containers being connected to an outside of the culture container.
-
公开(公告)号：US20250019419A1
公开(公告)日：2025-01-16
申请号：US18898929
申请日：2024-09-27
Applicant: FUJIFILM Corporation
Inventor： Shinichi NAKAI , Atsushi INADA , Hiroshi SAKUYAMA , Naoto TAKAHASHI
IPC: C07K16/00
Abstract: An object of the present invention is to provide a method in which the amount of mannose 5 in a product can be reduced in the production method for the product by cell culture, and a product that is produced by the method. According to the present invention, there is provided a production method for a product, including culturing cells in a culture container, in which the culturing is a perfusion culture method, Expressions (1) to (5) are satisfied in a production period of a product after reaching a target viable cell density, a variation of VCD during the production period is ±20% or less with respect to an average viable cell density during the production period, and a variation of VCV during the production period is ±20% or less with respect to an average value of the VCV during the production period. 0≤Gln-C≤2.5 Expression (1) 0.5≤Gluc-C≤8.0 Expression (2) 1.0≤NH4-C≤8.0 Expression (3) 50≤VCD≤300 Expression (4) 0.6×105≤VCV≤4.0×105 Expression (5) The meanings of the symbols in the expressions are as defined in the present specification.
-
-
-
Search Results
4
records
(took 0.011 seconds)
