公开(公告)号：US20250019419A1

公开(公告)日：2025-01-16

申请号：US18898929

申请日：2024-09-27

Applicant: FUJIFILM Corporation

Inventor： Shinichi NAKAI ,  Atsushi INADA ,  Hiroshi SAKUYAMA ,  Naoto TAKAHASHI

IPC: C07K16/00

Abstract: An object of the present invention is to provide a method in which the amount of mannose 5 in a product can be reduced in the production method for the product by cell culture, and a product that is produced by the method. According to the present invention, there is provided a production method for a product, including culturing cells in a culture container, in which the culturing is a perfusion culture method, Expressions (1) to (5) are satisfied in a production period of a product after reaching a target viable cell density, a variation of VCD during the production period is ±20% or less with respect to an average viable cell density during the production period, and a variation of VCV during the production period is ±20% or less with respect to an average value of the VCV during the production period. 0≤Gln-C≤2.5  Expression (1) 0.5≤Gluc-C≤8.0  Expression (2) 1.0≤NH4-C≤8.0  Expression (3) 50≤VCD≤300  Expression (4) 0.6×105≤VCV≤4.0×105  Expression (5) The meanings of the symbols in the expressions are as defined in the present specification.

公开(公告)号：US20220127593A1

公开(公告)日：2022-04-28

申请号：US17572011

申请日：2022-01-10

Applicant: FUJIFILM Corporation

Inventor： Yoichi NAGAI ,  Naoto TAKAHASHI ,  Michiko EDO

IPC: C12N13/00 ,  C12N15/85

Abstract: The producing method according to the disclosed technology is a producing method for an organism-derived material into which a bioactive substance has been introduced, the producing method comprising a step of causing a suspension containing the organism-derived material before the introduction of the bioactive substance and containing the bioactive substance to pass through a first electric field region having a first electric field intensity; and a step of causing the suspension to pass through a second electric field region having a second electric field intensity lower than the first electric field intensity after the suspension has passed through the first electric field region. The organism-derived material is a human-derived cell.

公开(公告)号：US20250011706A1

公开(公告)日：2025-01-09

申请号：US18894014

申请日：2024-09-24

Applicant: FUJIFILM CORPORATION

Inventor： Shinichi NAKAI ,  Hiroshi SAKUYAMA ,  Naoto TAKAHASHI

IPC: C12M1/36 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34

Abstract: A method including: connecting, through a coupling line, a culture section where a culture step, in which cells are cultured in a culture liquid stored in a culture tank and the cells are removed from the culture liquid to obtain a culture supernatant liquid containing a product of the cells, is performed and a purification section where a purification step, in which the product is purified from the culture supernatant liquid to obtain an active pharmaceutical ingredient of a biopharmaceutical, is performed, to allow the culture supernatant liquid to flow into the purification section from the culture section through the coupling line; connecting a flexible single-use tank having an accommodation capacity of 0.2 times to 10 times an amount of the culture liquid in the culture tank to a branch line provided between the culture section and the purification section; and temporarily storing the culture supernatant liquid in the tank.

公开(公告)号：US20240002784A1

公开(公告)日：2024-01-04

申请号：US18468223

申请日：2023-09-15

Applicant: FUJIFILM Corporation

Inventor： Atsushi INADA ,  Kosuke TANIGUCHI ,  Naoto TAKAHASHI

IPC: C12N5/00 ,  C12M1/34

CPC classification number: C12N5/0018 ,  C12M41/42 ,  C12N2500/02

Abstract: An object to be achieved by the present invention is to provide a culture method for cells, which makes it possible to suppress oxygen deficiency of cells in a case where the cell is cultured at a high density, and a production method for a useful substance using the culture method for cells. According to the present invention, there is provided a culture method for cells, in which a relationship between a stirring power P/V per unit volume, a dissolved oxygen concentration CL0, and a viable cell density VCD satisfies a condition of Expression 1, in cell culture in which the viable cell density is 60×106 cells/mL or more.


0.0059×exp[0.0243×(P/V)]×CL0/VCD≥1.10×10−17   Expression 1:

In the expression, L0 indicates a dissolved oxygen concentration [mol/mL]; VCD indicates a viable cell density [cells/mL]; and P/V indicates a stirring power [W/m3] per unit volume, which is defined by a predetermined expression.

公开(公告)号：US20210355430A1

公开(公告)日：2021-11-18

申请号：US17387091

申请日：2021-07-28

Applicant: FUJIFILM Corporation

Inventor： Naoto TAKAHASHI ,  Shinichi NAKAI ,  Hiroshi SAKUYAMA

IPC: C12N1/02 ,  C12M1/00 ,  C12N5/071

Abstract: In the cell culture device, a volume of a space defined by a diaphragm of the diaphragm pump and a plane at an outer edge of the diaphragm is 1 cm3 or more and 20 cm3 or less, in a case where an angle formed by a straight line connecting a foot of a perpendicular line from the apex to the plane to each point in the diaphragm and by the perpendicular line is denoted by an angle A, a thickness H of the diaphragm is within a range of 0.5 mm or more and 1.5 mm or less where the angle A is from 0° to 75°, and in a case where a thickness of the diaphragm at the apex is denoted by HT, the thickness H satisfies a relationship of 1≤HT/H≤1.75 at any point where the angle A is from 0° to 75°.

公开(公告)号：US20240336671A1

公开(公告)日：2024-10-10

申请号：US18743257

申请日：2024-06-14

Applicant: FUJIFILM Corporation

Inventor： Hiroshi SAKUYAMA ,  Naoto TAKAHASHI

IPC: C07K16/00 ,  C12M1/00 ,  C12M1/06 ,  C12M1/21

CPC classification number: C07K16/00 ,  C12M27/02 ,  C12M29/10 ,  C12M29/20 ,  C12M41/02 ,  C07K2317/14

Abstract: An object of the present invention is to provide a method for producing a product by cell culture, which makes it possible to suppress the clogging of an exhaust filter due to bubbles generated on an upper surface of a culture solution, and a cell culture device that makes it possible to suppress the clogging of an exhaust filter due to bubbles generated on an upper surface of a culture solution. According to the present invention, there is provided a method for producing a product, which includes culturing a cell using a cell culture device to produce a product from the cell, where the cell culture device includes a culture container accommodating a cell suspension containing cells and at least one or more bubble trap containers capable of accumulating bubbles, the one or more bubble trap containers being connected to an outside of the culture container.

公开(公告)号：US20200216792A1

公开(公告)日：2020-07-09

申请号：US16807740

申请日：2020-03-03

Applicant: FUJIFILM Corporation

Inventor： Shinichi NAKAI ,  Naoto TAKAHASHI

IPC: C12M1/42 ,  C12M1/36 ,  C12M1/00 ,  C12M1/26

Abstract: A method for producing a product related to the specified technology includes adjusting the concentration of cells in a culture vessel to a value of from 3×107 cells/ml to 3×108 cells/ml; in a case in which the average diameter of single cells in the culture vessel is designated as A, adjusting the number proportion of cells having a single cell diameter of 1.4×A or greater in the culture vessel to 5% or less, and adjusting the number proportion of cells having a single cell diameter in the range of A±A/7 to 50% or more.