公开(公告)号：US20150152400A1

公开(公告)日：2015-06-04

申请号：US14408612

申请日：2013-06-10

Applicant: IFP ENERGIES NOUVELLES

Inventor： Fadhel Ben Chaabane ,  Sylvain Louret

IPC: C12N9/24

CPC classification number: C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12P21/00 ,  C12P2203/00 ,  C12Y302/01004 ,  Y02E50/16

Abstract: A process for the production of an enzymatic cocktail with a cellulolytic microorganism comprises two phases:a phase a) for growth of said microorganism in a closed reactor in the presence of a carbonaceous growth solution;a phase b) for the production of said enzymatic cocktail carried out with a supply of carbonaceous production solution the concentration of carbonaceous substrate of which is in the range 150 to 400 g/L, said carbonaceous production solution comprising a carbonaceous inducer substrate;characterized in that said carbonaceous inducer substrate is a liquid residue obtained from a step for pre-treatment of lignocellulosic materials, the C5 sugar oligomers of which represent at least 1% by weight of the total sugars present in said liquid residue, and at least 0.3% by weight of the total sugars present in said carbonaceous production solution.

Abstract translation: 用纤维素分解微生物生产酶促混合物的方法包括两个阶段：一个阶段a）用于在碳质生长溶液存在下在封闭反应器中生长所述微生物; a）阶段b），用于生产所述酶促混合物，其中碳质生物溶液的碳浓度为150-400g / L，所述含碳生产溶液包含碳质诱导剂底物; 其特征在于，所述含碳诱导剂底物是由用于预处理木质纤维素材料的步骤获得的液体残余物，所述木质纤维素材料的C5糖低聚物占所述液体残余物中存在的总糖的至少1重量％，并且至少0.3 占所述碳质生产溶液中存在的总糖的重量％。

公开(公告)号：US09187770B2

公开(公告)日：2015-11-17

申请号：US14197619

申请日：2014-03-05

Applicant: IFP ENERGIES NOUVELLES

Inventor： Caroline Aymard ,  Pierre Antoine Bouillon ,  Stephanie Fleurier ,  Sylvain Louret ,  Larissa Perotta ,  Eszter Toth

IPC: C12P7/16 ,  C12P19/14 ,  C12P19/02 ,  C12P1/00 ,  C12N1/21 ,  C12M1/00 ,  A23K1/06 ,  A61K35/66 ,  A61P43/00 ,  C12P7/10 ,  C12P7/14 ,  C12P7/24 ,  C12P7/62 ,  C12P7/06 ,  C07H21/04

CPC classification number: C12P7/14 ,  C12P7/10 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/17

Abstract: The process for the production of alcohol and/or solvent from a biomass feedstock comprises the stages for pretreatment (P) of the biomass feedstock, for enzymatic hydrolysis (H) of the pretreated substrate, and for fermenting the hydrolyzate (F).To reduce the size of the fermenters, at least a portion of the solid residue contained in the hydrolyzate is extracted (Ex1) in such a way as to obtain a stream of solid residue (9) comprising lignin and a hydrolyzate (8) that is low in solid residue. Then, the stream of solid residue is washed (L) with a liquid stream in such a way as to recover a sugar-enriched liquid stream (15). The sugar-enriched liquid stream (15) is recycled in the enzymatic hydrolysis stage (H) to be able to upgrade the sugars without providing dilution of the streams in the process.

Abstract translation: 从生物质原料生产醇和/或溶剂的方法包括生物质原料的预处理（P），预处理底物的酶水解（H）和发酵水解产物（F）的阶段。 为了减小发酵罐的尺寸，提取水解产物中含有的至少一部分固体残余物（Ex1），以获得包含木质素和水解产物（8）的固体残余物（9），即 固体残渣少。 然后，用液体流洗涤固体残余物流（L），以回收富含糖的液体流（15）。 富含糖的液体流（15）在酶水解阶段（H）中再循环，以便能够在不提供该过程中的流的稀释的情况下升级糖。

公开(公告)号：US20140377812A1

公开(公告)日：2014-12-25

申请号：US14364081

申请日：2012-11-29

Applicant: IFP ENERGIES NOUVELLES

Inventor： Sylvain Louret ,  Romain Rousset

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  Y02E50/16

Abstract: The present invention concerns a production of liquefied lignocellulosic substrate by enzymatic reaction. 10% to 40% by weight dry matter of pre-treated lignocellulosic substrate is contacted, with water and enzymes at 0.1 to 60 mg of enzymes per gram of cellulose for a period of 1 to 24 hours. Over time, at least the value of one of the rheological characteristics of the reaction medium is measured. If a reduction of the value is detected over time, the following step a) is carried out: a) the feeding flow rate of pre-treated lignocellulosic substrate is increased, with or without modification of the flow rate of enzymes and/or water; If an increase of the value is detected over time, a step b) is carried out: b) the feeding flow rate of water and/or enzymes is increased, with or without modification of the flow rate of pre-treated lignocellulosic substrate.

Abstract translation: 本发明涉及通过酶反应生产液化的木质纤维素底物。 将预处理的木质纤维素底物的10重量％至40重量％的干物质与水和酶以0.1至60毫克酶/克纤维素接触1至24小时。 随着时间的推移，至少测量反应介质的流变特性之一的值。 如果随着时间的推移检测到值的降低，则执行以下步骤a）：a）预处理的木质纤维素底物的进料流速增加，伴随或不改变酶和/或水的流速; 如果随时间检测到值的增加，则进行步骤b）：b）在预处理的木质纤维素底物的流速或不改变的情况下，水和/或酶的进料流速增加。

公开(公告)号：US10150980B2

公开(公告)日：2018-12-11

申请号：US14409207

申请日：2013-06-05

Applicant: IFP Energies nouvelles

Inventor： Fadhel Ben Chaabane ,  Sylvain Louret

IPC: C12P19/14 ,  C12N1/00 ,  C12N9/42 ,  C12N9/96 ,  C12P7/10 ,  C12N1/36 ,  C12P19/02 ,  C13K1/02

Abstract: The invention concerns a process for deactivating a cellulolytic microorganism enabling the production of an enzymatic cocktail, said cocktail being used without separating the cellulolytic microorganism during the biochemical conversion of lignocellulosic materials, comprising at least one step for bringing a gaseous stream into contact with a medium containing said microorganism, said gaseous stream comprising more than 25% by weight of CO2 and comprising less than 0.5 molar % of O2.

公开(公告)号：US10030236B2

公开(公告)日：2018-07-24

申请号：US14408612

申请日：2013-06-10

Applicant: IFP ENERGIES NOUVELLES

Inventor： Fadhel Ben Chaabane ,  Sylvain Louret

IPC: C12N9/24 ,  C12P21/00 ,  C12N9/42

Abstract: A process for the production of an enzymatic cocktail with a cellulolytic microorganism comprises two phases: a phase a) for growth of said microorganism in a closed reactor in the presence of a carbonaceous growth solution; a phase b) for the production of said enzymatic cocktail carried out with a supply of carbonaceous production solution the concentration of carbonaceous substrate of which is in the range 150 to 400 g/L, said carbonaceous production solution comprising a carbonaceous inducer substrate; characterized in that said carbonaceous inducer substrate is a liquid residue obtained from a step for pre-treatment of lignocellulosic materials, the C5 sugar oligomers of which represent at least 1% by weight of the total sugars present in said liquid residue, and at least 0.3% by weight of the total sugars present in said carbonaceous production solution.

公开(公告)号：US09885027B2

公开(公告)日：2018-02-06

申请号：US14365274

申请日：2012-11-27

Applicant: IFP Energies nouvelles

Inventor： Fadhel Ben Chaabane ,  Sylvain Louret

IPC: C12N9/42 ,  C12N1/16 ,  C12N9/24 ,  C12P7/10 ,  C12P21/00

CPC classification number: C12N9/2437 ,  C12N1/16 ,  C12N9/2477 ,  C12P7/10 ,  C12P21/00 ,  C12P2203/00 ,  C12Y302/01004 ,  C12Y302/01074 ,  Y02E50/16

Abstract: The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases: a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L; a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.

公开(公告)号：US09605282B2

公开(公告)日：2017-03-28

申请号：US14772911

申请日：2014-02-06

Applicant: IFP ENERGIES NOUVELLES

Inventor： Caroline Aymard ,  Pierre-Antoine Bouillon ,  Stephanie Fleurier ,  Sylvain Louret ,  Larissa Perotta ,  Eszter Toth

IPC: C12P7/14 ,  C12P7/10

CPC classification number: C12P7/14 ,  C12P7/10 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/16

Abstract: The process for the production of alcohol and/or solvent from a biomass feedstock comprises the stages for pretreatment (P) of the biomass feedstock, for enzymatic hydrolysis (H1 and HF), and for fermenting the hydrolyzate (HF). To prevent solids from being sent and to facilitate operating the section for purifying the fermentation products, at least a portion of the solid material in the fermentation wine is extracted (Ex1) to obtain a stream of solid residue (11) comprising lignin and a fermentation wine (12) that is low in solid material. Then, the stream of solid residue is washed (L) with a liquid stream to recover a liquid stream that is enriched with fermentation products (16). The liquid stream that is enriched with fermentation products (16) is recycled in the enzymatic hydrolysis stage (H1) to recover any fermentation product and to increase the overall yield of the process.

公开(公告)号：US09593355B2

公开(公告)日：2017-03-14

申请号：US14364081

申请日：2012-11-29

Applicant: IFP ENERGIES NOUVELLES

Inventor： Sylvain Louret ,  Romain Rousset

IPC: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02

CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  Y02E50/16

Abstract: The present invention concerns a production of liquefied lignocellulosic substrate by enzymatic reaction. 10% to 40% by weight dry matter of pre-treated lignocellulosic substrate is contacted, with water and enzymes at 0.1 to 60 mg of enzymes per gram of cellulose for a period of 1 to 24 hours. Over time, at least the value of one of the rheological characteristics of the reaction medium is measured. If a reduction of the value is detected over time, the following step a) is carried out: a) the feeding flow rate of pre-treated lignocellulosic substrate is increased, with or without modification of the flow rate of enzymes and/or water; If an increase of the value is detected over time, a step b) is carried out: b) the feeding flow rate of water and/or enzymes is increased, with or without modification of the flow rate of pre-treated lignocellulosic substrate.

Abstract translation: 本发明涉及通过酶反应生产液化的木质纤维素底物。 将预处理的木质纤维素底物的10重量％至40重量％的干物质与水和酶以0.1至60毫克酶/克纤维素接触1至24小时。 随着时间的推移，至少测量反应介质的流变特性之一的值。 如果随着时间的推移检测到值的降低，则执行以下步骤a）：a）预处理的木质纤维素底物的进料流速增加，伴随或不改变酶和/或水的流速; 如果随时间检测到值的增加，则进行步骤b）：b）在预处理的木质纤维素底物的流速或不改变的情况下，水和/或酶的进料流速增加。

公开(公告)号：US20150191757A1

公开(公告)日：2015-07-09

申请号：US14409207

申请日：2013-06-05

Applicant: IFP Energies nouvelles

Inventor： Fadhel Ben Chaabane ,  Sylvain Louret

IPC: C12P19/14 ,  C13K1/02 ,  C12N1/36 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12N1/005 ,  C12N1/36 ,  C12N9/2437 ,  C12N9/96 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  Y02E50/16

Abstract: The invention concerns a process for deactivating a cellulolytic microorganism enabling the production of an enzymatic cocktail, said cocktail being used without separating the cellulolytic microorganism during the biochemical conversion of lignocellulosic materials, comprising at least one step for bringing a gaseous stream into contact with a medium containing said microorganism, said gaseous stream comprising more than 25% by weight of CO2 and comprising less than 0.5 molar % of O2.

Abstract translation: 本发明涉及使木质纤维素材料的生物化学转化期间不分离纤维素分解微生物的所述混合物在使用能够产生酶促混合物的情况下使解纤维素分解微生物失活的方法，该方法包括至少一个使气流与培养基接触的步骤 含有所述微生物，所述气流包含大于25重量％的CO 2并且包含小于0.5摩尔％的O 2。

公开(公告)号：US20140349373A1

公开(公告)日：2014-11-27

申请号：US14365274

申请日：2012-11-27

Applicant: IFP Energies nouvelles

Inventor： Fadhel Ben Chaabane ,  Sylvain Louret

IPC: C12N9/42

CPC classification number: C12N9/2437 ,  C12N1/16 ,  C12N9/2477 ,  C12P7/10 ,  C12P21/00 ,  C12P2203/00 ,  C12Y302/01004 ,  C12Y302/01074 ,  Y02E50/16

Abstract: The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases: a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L; a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.

Abstract translation: 本发明涉及通过用纤维素分解微生物浸没培养生产酶促混合物的方法，该方法包括两相：一相a）用于在封闭反应器中在至少一个含碳生长基质存在下生长所述微生物，所述相 生长期以含碳生长基质浓度在10〜90g / L范围内进行; b）用于生产其中提供至少一个含碳诱导剂底物的酶混合物，所述碳质诱导剂底物是从已经经过预处理的木质纤维素材料的酶水解步骤获得的至少一种固体残余物 所述生产阶段的碳质生产基质的浓度在150至400g / L的范围内进行。