公开(公告)号：US20240361321A1

公开(公告)日：2024-10-31

申请号：US18685111

申请日：2022-08-23

Applicant: INSTITUT PASTEUR ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

Inventor： Thierry ROSE ,  Sophie GOYARD ,  Yves JANIN ,  Pierre LAFAYE ,  Gabriel AYME ,  Nicolas ESCRIOU ,  Marion GRANSAGNE

IPC: G01N33/569 ,  C07K16/10 ,  C12Q1/66 ,  G01N33/68

CPC classification number: G01N33/56988 ,  C07K16/1003 ,  C07K16/1054 ,  C12Q1/66 ,  G01N33/6854 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2319/61 ,  G01N2333/161 ,  G01N2333/165 ,  G01N2333/90241

Abstract: System for detecting an antigen comprising: —a first fusion protein with no luciferase activity comprising: —a N-terminal domain which comprises a first single domain antibody which is directed against a first epitope of said antigen and —a C-terminal domain which comprises a first fragment of a luciferase having the amino acid sequence SEQ ID NO: 1 or is a variant thereof and —a second fusion protein with no luciferase activity comprising: —a N-terminal domain which comprises a second single domain antibody which is directed against a second epitope of said antigen and —a C-terminal domain which comprises a second fragment of a luciferase having the amino acid sequence SEQ ID NO: 2 or is a variant thereof. Luminescence is emitted in the presence of a substrate when both the first fusion protein and the second fusion protein bind to said antigen.

公开(公告)号：US20230145894A1

公开(公告)日：2023-05-11

申请号：US17996861

申请日：2021-04-26

Applicant: INSTITUT PASTEUR

Inventor： Thierry Rose ,  Sophie GOYARD ,  Laurent Lionel REBER ,  Yves JANIN

IPC: C07K16/42 ,  C12N9/02 ,  G01N33/535 ,  G01N33/533 ,  G01N33/577

CPC classification number: C07K16/4291 ,  C12N9/0069 ,  G01N33/535 ,  G01N33/533 ,  G01N33/577 ,  C07K2317/22 ,  C07K2317/622

Abstract: The present invention relates to a fusion protein comprising: —a N-terminal domain which comprises an antibody which is a variable domain of a camelid heavy-chain antibody (VHH) or a single chain variable fragment (scFV) and which is directed against an immunoglobulin and —a C-terminal domain which comprises a polypeptide with a luciferase activity: —having the amino acid sequence SEQ ID NO: 1 or —having at least 80% amino acid sequence identity to the amino acid sequence SEQ ID NO: 1.