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    (1→3, 1→4)—&bgr;-glucanase of enhanced stability
    1.
    发明授权
    (1→3, 1→4)—&bgr;-glucanase of enhanced stability 失效
    （1-> 3，1→4）β-葡聚糖酶

    公开(公告)号：US06277615B1

    公开(公告)日：2001-08-21

    申请号：US08584008

    申请日：1996-01-11

    申请人： Joseph Noozhumurry Varghese ,  Thomas Peter John Garrett ,  Geoffrey Bruce Fincher ,  Peter Bordier Hoj ,  Lin Chen

    发明人： Joseph Noozhumurry Varghese ,  Thomas Peter John Garrett ,  Geoffrey Bruce Fincher ,  Peter Bordier Hoj ,  Lin Chen

    IPC分类号： C12N924

    CPC分类号： C12Y302/01073 ,  A23K10/14 ,  C12N9/244 ,  C12N9/2448 ,  C12N15/8242 ,  C12Y302/01006

    摘要： This invention relates to a (1→3, 1→4)-&bgr;-glucanase (glucanase EII endohydrolase) enzyme, whose amino acid sequence has been modified in order to provide an enzyme whose three-dimensional structure confers improved thermostability and/or pH stability. Specific modifications are based upon a comparison between the three-dimensional structure (1→3, 1→4)-&bgr;-glucanase and that of (1→3)-&bgr;-glucanase. The (1→3, 1→4)-&bgr;-glucanase gene has been modified by site-directed mutagenesis, and modified enzymes have been expressed in E. coli. Modified sequences, DNA molecules encoding them, plasmids, expression vectors and transgenic plants are disclosed.

    摘要翻译： 本发明涉及一种已经修饰其氨基酸序列的（1-> 3,1-4）β-葡聚糖酶（葡聚糖酶EII内切水解酶）酶，以提供其三维结构赋予改善的热稳定性和/ 或pH稳定性。 具体的修饰是基于三维结构（1-> 3,1→4）-β-葡聚糖酶和（1-> 3）-β-葡聚糖酶的比较。 （1-> 3，1→4）β-葡聚糖酶基因已经通过定点诱变进行了修饰，修饰的酶已经在大肠杆菌中表达。 公开了修饰序列，编码它们的DNA分子，质粒，表达载体和转基因植物。

    POLYSACCHARIDE TRANSFERASE
    2.
    发明申请
    POLYSACCHARIDE TRANSFERASE 审中-公开
    多糖转移

    公开(公告)号：US20100009414A1

    公开(公告)日：2010-01-14

    申请号：US12375237

    申请日：2007-07-27

    申请人： Maria Hrmova ,  Geoffrey Bruce Fincher

    发明人： Maria Hrmova ,  Geoffrey Bruce Fincher

    IPC分类号： C12P19/04 ,  C12N9/10 ,  C07H1/00 ,  C12N5/04

    CPC分类号： C12N9/1048 ,  C12N9/1051 ,  C12Y204/01074 ,  C12Y204/01207

    摘要： The present invention is predicated on the discovery that a polysaccharide transferase purified from barley seedlings can catalyze the formation of covalent bonds between different polysaccharides, including between xyloglucans and cellulose, and between xyloglucans and (1,3;1,4)-β-D-glucans. The present invention thus provides, among other things, an isolated or substantially purified polysaccharide transferase, wherein said polysaccharide transferase is capable of catalyzing the formation of a covalent bond between a donor polysaccharide and an acceptor polysaccharide; or a functionally active fragment or variant of said polysaccharide transferase.

    摘要翻译： 本发明基于以下发现：从大麦幼苗纯化的多糖转移酶可以催化不同多糖（包括木葡聚糖和纤维素之间）和木葡聚糖与（1,3; 1,4）-β-D 葡聚糖 因此，本发明尤其提供了分离或基本上纯化的多糖转移酶，其中所述多糖转移酶能够催化供体多糖和受体多糖之间的共价键的形成; 或所述多糖转移酶的功能活性片段或变体。

    POLYSACCHARIDE TRANSFERASE
    3.
    发明申请
    POLYSACCHARIDE TRANSFERASE 审中-公开
    多糖转移

    公开(公告)号：US20120083036A1

    公开(公告)日：2012-04-05

    申请号：US13325976

    申请日：2011-12-14

    申请人： Maria Hrmova ,  Geoffrey Bruce Fincher

    发明人： Maria Hrmova ,  Geoffrey Bruce Fincher

    IPC分类号： C12N15/01 ,  C12N5/04 ,  C12N15/82 ,  C12N9/99 ,  C12N9/10

    CPC分类号： C12N9/1048 ,  C12N9/1051 ,  C12Y204/01074 ,  C12Y204/01207

    摘要： The present invention is predicated on the discovery that a polysaccharide transferase purified from barley seedlings can catalyze the formation of covalent bonds between different polysaccharides, including between xyloglucans and cellulose, and between xyloglucans and (1,3;1,4)-β-D-glucans. The present invention thus provides, among other things, an isolated or substantially purified polysaccharide transferase, wherein said polysaccharide transferase is capable of catalyzing the formation of a covalent bond between a donor polysaccharide and an acceptor polysaccharide; or a functionally active fragment or variant of said polysaccharide transferase.

    摘要翻译： 本发明基于以下发现：从大麦幼苗纯化的多糖转移酶可以催化不同多糖（包括木葡聚糖和纤维素之间）和木葡聚糖与（1,3; 1,4） - D-葡聚糖。 因此，本发明尤其提供了分离或基本上纯化的多糖转移酶，其中所述多糖转移酶能够催化供体多糖和受体多糖之间的共价键的形成; 或所述多糖转移酶的功能活性片段或变体。