公开(公告)号：US20220002716A1

公开(公告)日：2022-01-06

申请号：US17364956

申请日：2021-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Anilkumar KORE ,  Senthilvelan ANNAMALAI ,  Shanmugasundaram MUTHIAN ,  Robert POTTER ,  Tyson VONDERFECHT

IPC: C12N15/11 ,  C07H21/00 ,  C12N9/12 ,  A61K31/7088 ,  A61K47/54 ,  A61K38/45

Abstract: This specification generally relates to trinucleotide RNA cap analogs, methods of use thereof, and kits comprising same. In particular, the trinucleotide cap analogs provided herein permit ready detection and/or isolation of capped RNA transcripts in vitro and translation of capped mRNAs in vivo.

公开(公告)号：US20160281095A1

公开(公告)日：2016-09-29

申请号：US15178821

申请日：2016-06-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Anilkumar KORE ,  Shanmugasundaram MUTHIAN ,  Kyle GEE

IPC: C12N15/67 ,  C07C323/60 ,  C12P19/34 ,  A61K39/39 ,  A61K39/12 ,  C12N7/00 ,  C12N15/11 ,  C07D213/82

CPC classification number: C12N15/67 ,  A61K31/7084 ,  A61K31/7088 ,  A61K31/7115 ,  A61K31/712 ,  A61K39/12 ,  A61K39/39 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/55511 ,  C07C247/04 ,  C07C323/60 ,  C07D213/82 ,  C07H1/00 ,  C07H1/02 ,  C07H1/04 ,  C07H19/207 ,  C07H21/02 ,  C12N7/00 ,  C12N15/11 ,  C12N2770/36134 ,  C12N2770/36171 ,  C12P19/34 ,  C12Q1/68

Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.

公开(公告)号：US20150252373A1

公开(公告)日：2015-09-10

申请号：US14600155

申请日：2015-01-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Anilkumar KORE ,  Shanmugasundaram Muthian ,  Kyle Gee

IPC: C12N15/67 ,  C07D213/82 ,  C12N15/11 ,  C07C247/04

CPC classification number: C12N15/67 ,  A61K31/7084 ,  A61K31/7088 ,  A61K31/7115 ,  A61K31/712 ,  A61K39/12 ,  A61K39/39 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/55511 ,  C07C247/04 ,  C07C323/60 ,  C07D213/82 ,  C07H1/00 ,  C07H1/02 ,  C07H1/04 ,  C07H19/207 ,  C07H21/02 ,  C12N7/00 ,  C12N15/11 ,  C12N2770/36134 ,  C12N2770/36171 ,  C12P19/34 ,  C12Q1/68

Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.

Abstract translation: 提供炔基衍生的帽类似物，炔基修饰的封端RNA，1,4-二取代的三唑衍生的封端RNA，制备方法，分离方法及其用途。 “点击”修改有助于检测和分离封闭的RNA，通过“点击”反应形成的1,4-二取代的三唑衍生物可用于产生RNA转录物和编码的蛋白质。