公开(公告)号：US12152256B2

公开(公告)日：2024-11-26

申请号：US17932844

申请日：2022-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US10344325B2

公开(公告)日：2019-07-09

申请号：US14937211

申请日：2015-11-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven Menchen ,  Theo Nikiforov ,  Barnett Rosenblum

IPC: C12Q1/68 ,  C12Q1/6869 ,  G01N27/414

Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.

公开(公告)号：US10597642B2

公开(公告)日：2020-03-24

申请号：US15690945

申请日：2017-08-30

Applicant: Life Technologies Corporation

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6804 ,  C12Q1/6818

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US09765310B2

公开(公告)日：2017-09-19

申请号：US14991230

申请日：2016-01-08

Applicant: Life Technologies Corporation

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US09399767B2

公开(公告)日：2016-07-26

申请号：US14191997

申请日：2014-02-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marian Peris ,  Michael Phelan ,  Barnett Rosenblum ,  Stephen Hendricks

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

Abstract translation: 本文提供了衍生自野生型RB69 DNA聚合酶或与野生型RB69 DNA聚合酶相关的突变型DNA依赖性聚合酶。 这些突变型聚合酶能够选择性地结合标记的核苷酸。 这些突变型聚合酶还能够掺入多种天然存在和修饰的核苷酸，包括例如终止子核苷酸。

公开(公告)号：US20250163391A1

公开(公告)日：2025-05-22

申请号：US18952663

申请日：2024-11-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12N9/12 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US20160208318A1

公开(公告)日：2016-07-21

申请号：US14991230

申请日：2016-01-08

Applicant: Life Technologies Corporation

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

Abstract translation: 本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。 该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。 该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。 标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。 所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

公开(公告)号：US11447756B2

公开(公告)日：2022-09-20

申请号：US16825788

申请日：2020-03-20

Applicant: Life Technologies Corporation

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6804 ,  C12Q1/6818

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US11047004B2

公开(公告)日：2021-06-29

申请号：US16421284

申请日：2019-05-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven Menchen ,  Theo Nikiforov ,  Barnett Rosenblum

IPC: C12Q1/6869 ,  G01N27/414

Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.

公开(公告)号：US10336991B2

公开(公告)日：2019-07-02

申请号：US15422224

申请日：2017-02-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marian Peris ,  Michael Phelan ,  Barnett Rosenblum ,  Stephen Hendricks

IPC: C12N9/12 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/68

Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.