公开(公告)号：US09417233B2

公开(公告)日：2016-08-16

申请号：US14602619

申请日：2015-01-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Aldrich Lau ,  Robert Eason ,  Maxim Brevnov ,  Handong Li ,  Kevin Hacker

IPC: C12Q1/70 ,  C12Q1/68 ,  G01N33/543 ,  C12N1/02 ,  G01N33/569 ,  C12N13/00

CPC classification number: G01N33/54326 ,  C12N1/02 ,  C12N13/00 ,  C12Q1/689 ,  G01N33/54353 ,  G01N33/56911

Abstract: Magnetic beads that include polyvalent ligands comprising various carbohydrates are described. Methods for fabricating such magnetic beads are also provided as well as methods of their use to capture and enrich pathogen cell population for subsequent culture, lysis and identification.

公开(公告)号：US09988622B2

公开(公告)日：2018-06-05

申请号：US14697962

申请日：2015-04-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kevin Hacker ,  Gregory Govoni ,  Nikolay Sergeev ,  Elena Bolchakova ,  Maxim Brevnov ,  Manohar Furtado ,  Johnie Young ,  James Nurse ,  Mariela Cuadras

IPC: C12N15/10 ,  C12Q1/68 ,  G01N21/75 ,  C12N1/06 ,  B01D29/13

CPC classification number: C12N15/1003 ,  B01D29/13 ,  C12N1/066 ,  C12Q1/68 ,  C12Q1/689 ,  C12Q1/6895 ,  G01N21/75

Abstract: Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.

公开(公告)号：US20150197743A1

公开(公告)日：2015-07-16

申请号：US14602619

申请日：2015-01-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Aldrich Lau ,  Robert Eason ,  Maxim Brevnov ,  Handong Li ,  Kevin Hacker

IPC: C12N13/00 ,  C12Q1/68

CPC classification number: G01N33/54326 ,  C12N1/02 ,  C12N13/00 ,  C12Q1/689 ,  G01N33/54353 ,  G01N33/56911

Abstract: Magnetic beads that include polyvalent ligands comprising various carbohydrates are described. Methods for fabricating such magnetic beads are also provided as well as methods of their use to capture and enrich pathogen cell population for subsequent culture, lysis and identification.

Abstract translation: 描述包括包含各种碳水化合物的多价配体的磁珠。 还提供了制备这种磁珠的方法以及它们用于捕获和富集病原体细胞群以用于随后的培养，裂解和鉴定的方法。