公开(公告)号：US20140005061A1

公开(公告)日：2014-01-02

申请号：US13790447

申请日：2013-03-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert TEBBS ,  Craig Cummings ,  Pius Brzoska ,  Sharon Matheny ,  Catherine O'Connell ,  Manohar Furtado ,  Nikolay Sergeev ,  Maxim Brevnov

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: The present disclosure describes compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as shiga toxin stx1 and stx2 and eae. Some embodiments relate to detection of STEC microorganisms including an E. coli O26, an E. coli O45, an E. coli O103, an E. coli O111, an E. coli O121 or an E. coli O145. In some embodiments, compositions, methods and kits can detect and identify individual serotypes of shiga toxin producing microorganisms. Workflows for multiple microbe detection and identification are also described.

Abstract translation: 本公开描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。 一些实施方案涉及检测产生毒力因子的一种或多种微生物，例如志贺毒素stx1和stx2和eae。 一些实施方案涉及检测STEC微生物，包括大肠杆菌O26，大肠杆菌O45，大肠杆菌O103，大肠杆菌O111，大肠杆菌O121或大肠杆菌O145。 在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体血清型。 还描述了用于多个微生物检测和鉴定的工作流程。

公开(公告)号：US09988622B2

公开(公告)日：2018-06-05

申请号：US14697962

申请日：2015-04-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kevin Hacker ,  Gregory Govoni ,  Nikolay Sergeev ,  Elena Bolchakova ,  Maxim Brevnov ,  Manohar Furtado ,  Johnie Young ,  James Nurse ,  Mariela Cuadras

IPC: C12N15/10 ,  C12Q1/68 ,  G01N21/75 ,  C12N1/06 ,  B01D29/13

CPC classification number: C12N15/1003 ,  B01D29/13 ,  C12N1/066 ,  C12Q1/68 ,  C12Q1/689 ,  C12Q1/6895 ,  G01N21/75

Abstract: Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.