公开(公告)号：US11453912B2

公开(公告)日：2022-09-27

申请号：US16364336

申请日：2019-03-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Melville Davey ,  Michael Meyer ,  Marcin Sikora ,  Simon Cawley ,  Kirk Pastorian

IPC: C12Q1/6874 ,  G16B30/00 ,  G16B99/00

Abstract: A method of estimating a parameter related to sequencing of a sample nucleic acid template includes: receiving signal data relating to nucleotide incorporation events resulting from a series of flows of nucleotides onto an array of wells including (i) a first well containing the sample nucleic acid template and (ii) a plurality of other sample-containing wells; determining sequence information for the sample nucleic acid template using signal data from the first well; and constructing a phase-state model for a set of nucleotide flows that contributed at least in part to the sequence information, wherein the model includes a signal correction parameter that is determined using signal data from the plurality of other sample-containing wells.

公开(公告)号：US10273540B2

公开(公告)日：2019-04-30

申请号：US13967665

申请日：2013-08-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Melville Davey ,  Michael Meyer ,  Marcin Sikora ,  Simon Cawley ,  Kirk Pastorian

IPC: G06F19/10 ,  C12Q1/6874

Abstract: A method of estimating a parameter related to sequencing of a sample nucleic acid template includes: receiving signal data relating to nucleotide incorporation events resulting from a series of flows of nucleotides onto an array of wells including (i) a first well containing the sample nucleic acid template and (ii) a plurality of other sample-containing wells; determining sequence information for the sample nucleic acid template using signal data from the first well; and constructing a phase-state model for a set of nucleotide flows that contributed at least in part to the sequence information, wherein the model includes a signal correction parameter that is determined using signal data from the plurality of other sample-containing wells.

公开(公告)号：US20230131684A1

公开(公告)日：2023-04-27

申请号：US17929782

申请日：2022-09-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Melville Davey ,  Michael Meyer ,  Marcin Sikora ,  Simon Cawley ,  Kirk Pastorian

IPC: C12Q1/6874 ,  G16B30/00

Abstract: A method of estimating a parameter related to sequencing of a sample nucleic acid template includes: receiving signal data relating to nucleotide incorporation events resulting from a series of flows of nucleotides onto an array of wells including (i) a first well containing the sample nucleic acid template and (ii) a plurality of other sample-containing wells; determining sequence information for the sample nucleic acid template using signal data from the first well; and constructing a phase-state model for a set of nucleotide flows that contributed at least in part to the sequence information, wherein the model includes a signal correction parameter that is determined using signal data from the plurality of other sample-containing wells.

公开(公告)号：US20140222399A1

公开(公告)日：2014-08-07

申请号：US14150855

申请日：2014-01-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Melville Davey ,  Michael Meyer

IPC: G06F19/12

CPC classification number: G16B5/00 ,  C12Q1/6874 ,  C12Q2537/165

Abstract: A method of obtaining a more accurate estimate of a signal correction parameter(s) in sequencing-by-synthesis operations, such as incomplete extension rates, carry forward rates, and/or signal droop rates. The sequencing operation produces signal data. A model is constructed to simulate a population of template strands as it undergoes the sequencing process and becomes divided into different phase-states as the sequencing-by-synthesis progresses. For example, the model may be a phase-state model. The output from the model is used to adjust the signal correction parameter(s). For example, the model may be fitted to the signal data. This fitting results in a more accurate estimate of the signal correction parameter(s). In another embodiment, the signal droop rate is modeled as a decaying function and this decaying function is fitted to the signal data to obtain an improved estimate of the signal droop rate.

Abstract translation: 一种在逐个合成操作中获得信号校正参数的更精确估计的方法，例如不完全扩展速率，结转率和/或信号下降率。 排序操作产生信号数据。 构建模型以模拟模板链的群体，因为其经历了测序过程，并且随着逐个序列合成的进行而分为不同的相态。 例如，模型可以是阶段状态模型。 模型的输出用于调整信号校正参数。 例如，该模型可以适合于信号数据。 该拟合导致信号校正参数的更精确的估计。 在另一个实施例中，信号下降率被建模为衰减函数，并且该衰减函数适合于信号数据以获得信号下降率的改进估计。