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    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    1.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20200277580A1

    公开(公告)日：2020-09-03

    申请号：US16850629

    申请日：2020-04-16

    Applicant: Life Technologies Corporation

    Inventor： Peter VANDER HORN ,  Theo NIKIFOROV ,  Guobin LUO ,  Mindy LANDES ,  Daniel MAZUR ,  Eileen TOZER ,  Tommie LINCECUM

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
    3.
    发明公开
    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME 审中-公开

    公开(公告)号：US20240101980A1

    公开(公告)日：2024-03-28

    申请号：US18521466

    申请日：2023-11-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel MAZUR ,  Sihong CHEN ,  Peter VANDER HORN ,  Eileen TOZER ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

    IPC: C12N9/12 ,  C12Q1/686

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    MODIFIED NUCLEOTIDES AND USES THEREOF
    5.
    发明申请
    MODIFIED NUCLEOTIDES AND USES THEREOF 审中-公开

    公开(公告)号：US20180119217A1

    公开(公告)日：2018-05-03

    申请号：US15561445

    申请日：2016-03-18

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES GMBH

    Inventor： Wolfgang HINZ ,  Steven MENCHEN ,  Ronald GRAHAM ,  Peter VANDER HORN ,  Earl HUBBELL ,  Christian WOEHLER ,  Roman ROZHKOV ,  Barnett ROSENBLUM

    IPC: C12Q1/6874

    CPC classification number: C12Q1/6874 ,  C12Q2565/607

    Abstract: In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    6.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20170260512A1

    公开(公告)日：2017-09-14

    申请号：US15481858

    申请日：2017-04-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    7.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20200299655A1

    公开(公告)日：2020-09-24

    申请号：US16894469

    申请日：2020-06-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    METHODS AND SYSTEMS FOR MODELING PHASING EFFECTS IN SEQUENCING USING TERMINATION CHEMISTRY
    8.
    发明申请
    METHODS AND SYSTEMS FOR MODELING PHASING EFFECTS IN SEQUENCING USING TERMINATION CHEMISTRY 审中-公开

    公开(公告)号：US20200043571A1

    公开(公告)日：2020-02-06

    申请号：US16550374

    申请日：2019-08-26

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Christian KOLLER ,  Marcin SIKORA ,  Peter VANDER HORN

    IPC: G16B40/10 ,  G16B5/00 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  C12Q1/6869

    Abstract: A method for nucleic acid sequencing includes receiving observed or measured nucleic acid sequencing data from a sequencing instrument that receives and processes a sample nucleic acid in a termination sequencing-by-synthesis process. The method also includes generating a set of candidate sequences of bases for the observed or measured nucleic acid sequencing data by determining a predicted signal for candidate sequences using a simulation framework. The simulation framework incorporates an estimated carry forward rate (CFR), an estimated incomplete extension rate (IER), an estimated droop rate (DR), an estimated reactivated molecules rate (RMR), and an estimated termination failure rate (TFR), the RMR being greater than or equal to zero and the TFR being lesser than one. The method also includes identifying, from the set of candidate sequences of bases, one candidate sequence leading to optimization of a solver function as corresponding to the sequence for the sample nucleic acid.

    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
    9.
    发明申请
    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME 审中-公开

    公开(公告)号：US20190270974A1

    公开(公告)日：2019-09-05

    申请号：US16415063

    申请日：2019-05-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel MAZUR ,  Peter VANDER HORN ,  Eileen TOZER ,  Sihong CHEN ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

    IPC: C12N9/12 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
    10.
    发明申请
    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME 审中-公开

    公开(公告)号：US20170096648A1

    公开(公告)日：2017-04-06

    申请号：US15277834

    申请日：2016-09-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： DANIEL MAZUR ,  Peter VANDER HORN ,  Eileen TOZER ,  Sihong CHEN ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

    IPC: C12N9/12

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q2521/101

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

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