公开(公告)号：US10081833B2

公开(公告)日：2018-09-25

申请号：US14856448

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu Chen ,  Ruoying Tan

IPC: C12Q1/68 ,  C12Q1/6858 ,  C12Q1/6883 ,  C12Q1/686

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161 ,  C12Q2525/186 ,  C12Q2565/107 ,  C12Q2535/125

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US11572585B2

公开(公告)日：2023-02-07

申请号：US15930101

申请日：2020-05-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu Chen ,  Ruoying Tan

IPC: C12Q1/6858 ,  C12Q1/6883 ,  C12Q1/686

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US10570459B2

公开(公告)日：2020-02-25

申请号：US15923954

申请日：2018-03-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu Chen ,  Ruoying Tan

IPC: C12Q1/68 ,  C12Q1/6886 ,  C12Q1/6858 ,  C07H21/04

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US11530450B2

公开(公告)日：2022-12-20

申请号：US16745296

申请日：2020-01-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu Chen ,  Ruoying Tan

IPC: C12Q1/6886 ,  C12Q1/6858 ,  C07H21/04

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20160040256A1

公开(公告)日：2016-02-11

申请号：US14879156

申请日：2015-10-09

Applicant: Life Technologies Corporation

Inventor： Caifu CHEN ,  Ruoying Tan

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C07H21/04 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/172 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US10689694B2

公开(公告)日：2020-06-23

申请号：US16109191

申请日：2018-08-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu Chen ,  Ruoying Tan

IPC: C12Q1/68 ,  C12Q1/6858 ,  C12Q1/6883 ,  C12Q1/686

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US09512473B2

公开(公告)日：2016-12-06

申请号：US14445046

申请日：2014-07-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu Chen ,  Ruoying Tan

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161 ,  C12Q2525/186 ,  C12Q2565/107 ,  C12Q2535/125

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

Abstract translation: 在一些实施方案中，本发明一般涉及用于区分不同等位基因之间的序列变异的组合物，方法和试剂盒。 更具体地说，在一些实施方案中，本发明提供了用于定量稀少（例如，突变体）等位基因变体如SNP或核苷酸（NT）插入或缺失的组合物，方法和试剂盒，其包含丰富的（例如，野生型 ）等位基因变体，具有高特异性和选择性。 特别地，在一些实施方案中，本发明涉及用于突变检测的高选择性方法，称为竞争性等位基因特异性TaqMan PCR（“cast-PCR”）。

公开(公告)号：US20150140557A1

公开(公告)日：2015-05-21

申请号：US14445046

申请日：2014-07-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying Tan

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161 ,  C12Q2525/186 ,  C12Q2565/107 ,  C12Q2535/125

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

Abstract translation: 在一些实施方案中，本发明一般涉及用于区分不同等位基因之间的序列变异的组合物，方法和试剂盒。 更具体地说，在一些实施方案中，本发明提供了用于定量稀少（例如，突变体）等位基因变体如SNP或核苷酸（NT）插入或缺失的组合物，方法和试剂盒，其包含丰富的（例如，野生型 ）等位基因变体，具有高特异性和选择性。 特别地，在一些实施方案中，本发明涉及用于突变检测的高选择性方法，称为竞争性等位基因特异性TaqMan PCR（“cast-PCR”）。