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    Methods, compositions, and kits for detecting allelic variants
    1.
    发明授权
    Methods, compositions, and kits for detecting allelic variants 有权

    公开(公告)号：US11572585B2

    公开(公告)日：2023-02-07

    申请号：US15930101

    申请日：2020-05-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Caifu Chen ,  Ruoying Tan

    IPC: C12Q1/6858 ,  C12Q1/6883 ,  C12Q1/686

    Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

    Small RNA Capture, Detection and Quantification
    4.
    发明申请
    Small RNA Capture, Detection and Quantification 有权
    小RNA捕获，检测和定量

    公开(公告)号：US20150105275A1

    公开(公告)日：2015-04-16

    申请号：US14233711

    申请日：2013-11-04

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Linda Wong ,  Caifu Chen ,  Yalei Wu ,  Shoulian Dong ,  Chunmei Liu

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/191 ,  C12Q2525/301

    Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

    Abstract translation: 本文提供了用于捕获，检测和定量成熟小RNA的方法，组合物和试剂盒。 方法的实施方案包括在5'和3'半简并连接夹板的存在下分别将5'和3'连接衔接子连接到成熟小RNA的5'和3'末端以产生连接产物。 其他实施方案包括具有通用逆转录引物的逆转录聚腺苷酸化成熟小RNA，并且在半退化连接夹板存在下将衔接子连接到cDNA的3'末端以产生cDNA连接产物。

    Methods, compositions, and kits for detecting allelic variants
    5.
    发明授权
    Methods, compositions, and kits for detecting allelic variants 有权

    公开(公告)号：US11530450B2

    公开(公告)日：2022-12-20

    申请号：US16745296

    申请日：2020-01-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Caifu Chen ,  Ruoying Tan

    IPC: C12Q1/6886 ,  C12Q1/6858 ,  C07H21/04

    Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

    Methods, compositions, and kits for detecting allelic variants
    9.
    发明授权
    Methods, compositions, and kits for detecting allelic variants 有权

    公开(公告)号：US10570459B2

    公开(公告)日：2020-02-25

    申请号：US15923954

    申请日：2018-03-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Caifu Chen ,  Ruoying Tan

    IPC: C12Q1/68 ,  C12Q1/6886 ,  C12Q1/6858 ,  C07H21/04

    Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

    Method of performing digital PCR
    10.
    发明授权
    Method of performing digital PCR 有权

    公开(公告)号：US10030262B2

    公开(公告)日：2018-07-24

    申请号：US14395456

    申请日：2013-04-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Caifu Chen ,  Casey McFarland ,  David Keys

    IPC: C12P19/34 ,  C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6858

    Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

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