公开(公告)号：US09986699B2

公开(公告)日：2018-06-05

申请号：US14629235

申请日：2015-02-23

Applicant: Monsanto Technology LLC

Inventor： Kevin L. Deppermann ,  Michael W. Petersen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather Forbes ,  Bruce Schnicker ,  John Tamulonis

IPC: A01H1/02 ,  A01H1/04 ,  A01H1/08 ,  A01H5/10 ,  C12N15/82 ,  C12Q1/68

CPC classification number: A01H1/02 ,  A01H1/04 ,  A01H1/08 ,  A01H5/10 ,  C12N15/8241 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: Novel methods are provided to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for introgressive hybridization, for example, generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, and analyzing nucleic acids extracted from the tissue samples for at least one genetic marker. The method then further includes selecting the sampled seeds that possess the at least one genetic marker, cultivating fertile plants from the selected seeds, and crossing the fertile plants with other plants.

公开(公告)号：US20150355205A1

公开(公告)日：2015-12-10

申请号：US14685047

申请日：2015-04-13

Applicant: Monsanto Technology LLC

Inventor： Kevin L. Deppermann ,  Michael W. Petersen ,  Allen N. Ondes ,  David W. Finley ,  William M. Fischer ,  John M. Jensen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather M. Forbes ,  Bruce Schnicker ,  John Tamulonis

IPC: G01N35/00 ,  G01N1/04 ,  G01N1/20 ,  G01N33/50 ,  G01N1/28 ,  A01C1/00 ,  G01N33/00

CPC classification number: G01N35/00584 ,  A01C1/00 ,  A01C1/025 ,  G01N1/04 ,  G01N1/20 ,  G01N1/286 ,  G01N33/0098 ,  G01N33/5097 ,  G01N2001/002

Abstract: A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

Abstract translation: 提供种子采样系统，其具有包括种子仓的自动种子加载组件，并且可操作以从种子仓内的多个种子中分离种子。 该系统还包括可操作以从单个种子中去除组织样本的自动种子取样组件，以及可操作以将单种化种子从种子装载组件转移到种子采样组件的自动种子运输组件。 种子运输组件包括多个保持构件。 每个保持构件可相对于种子装载组件和种子取样组件移动。 种子运输组件可操作以将多个保持构件中的一个定位成与种子装载组件相邻以接合单个种子之一，同时将另一个保持构件定位成与种子取样组件相邻，以将另一个单个种子呈现到 种子采样总成。

公开(公告)号：US12163196B2

公开(公告)日：2024-12-10

申请号：US17319657

申请日：2021-05-13

Applicant: Monsanto Technology LLC

Inventor： David Butruille ,  Kevin L. Deppermann ,  Stanton Dotson ,  Sam Eathington ,  Heather Forbes ,  Michael W. Petersen ,  Bruce Schnicker ,  John Tamulonis

IPC: C12Q1/6895 ,  A01H1/04 ,  A01H5/10 ,  A01H6/54 ,  C12N15/82

Abstract: A method is provided for use in analyzing seeds, in a population of seeds, for one or more desired characteristic. The method includes removing tissue from individual seeds in a population of seeds using an automated seed sampler, while preserving germination viability of the seeds, and analyzing the tissue for the presence or absence of one or more desired characteristic. The method then includes, based on the analysis of the tissue, quantifying the one or more desired characteristic for each of the individual seeds that possess the one or more desired characteristic.

公开(公告)号：US10705102B2

公开(公告)日：2020-07-07

申请号：US14685047

申请日：2015-04-13

Applicant: Monsanto Technology LLC

Inventor： Kevin L. Deppermann ,  Michael W. Petersen ,  Allen N. Ondes ,  David W. Finley ,  William M. Fischer ,  John M. Jensen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather M. Forbes ,  Bruce Schnicker ,  John Tamulonis

IPC: A01C1/00 ,  G01N35/00 ,  G01N33/00 ,  G01N33/50 ,  G01N1/20 ,  G01N1/28 ,  A01C1/02 ,  G01N1/04 ,  G01N1/00

Abstract: A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

公开(公告)号：US11006593B2

公开(公告)日：2021-05-18

申请号：US15996080

申请日：2018-06-01

Applicant: Monsanto Technology LLC

Inventor： David Butruille ,  Kevin L. Deppermann ,  Stanton Dotson ,  Sam Eathington ,  Heather Forbes ,  Michael W. Petersen ,  Bruce Schnicker ,  John Tamulonis

IPC: A01H1/02 ,  A01H1/04 ,  C12Q1/68 ,  A01H1/08 ,  A01H5/10 ,  C12N15/82 ,  C12Q1/6895

Abstract: A method is provided for use in a plant breeding program to generate a population having one or more desired traits. The method includes removing a tissue sample from each of one or more seeds using an automated seed sampler while preserving germination viability of the sampled seeds; analyzing the tissue samples for the presence or absence of a genetic sequence associated with at least one desired trait; based on the analysis of the tissue samples, cultivating plants from the sampled seeds that either possess or lack the genetic sequence associated with the at least one desired trait; creating a parental cross using at least one of the cultivated plants; and selecting seeds from the parental cross and cultivating offspring of the parental cross using the selected seeds.

公开(公告)号：US20150164011A1

公开(公告)日：2015-06-18

申请号：US14629235

申请日：2015-02-23

Applicant: Monsanto Technology LLC

Inventor： Kevin L. Deppermann ,  Michael W. Petersen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather Forbes ,  Bruce Schnicker ,  John Tamulonis

IPC: A01H1/02 ,  C12Q1/68 ,  A01H1/04

CPC classification number: A01H1/02 ,  A01H1/04 ,  A01H1/08 ,  A01H5/10 ,  C12N15/8241 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: Novel methods are provided to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for introgressive hybridization, for example, generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, and analyzing nucleic acids extracted from the tissue samples for at least one genetic marker. The method then further includes selecting the sampled seeds that possess the at least one genetic marker, cultivating fertile plants from the selected seeds, and crossing the fertile plants with other plants.

Abstract translation: 提供了新的方法，通过使用高通量，无损取样的种子来促进种质改良活动。 例如，侵入性杂交的方法通常包括使用自动化种子取样器从个体种子中除去组织样品，而不影响种子的萌发活力，以及分析从组织样品提取至少一种遗传标记的核酸。 该方法然后进一步包括选择具有至少一个遗传标记的采样种子，从所选择的种子培养可育植物，并将可育植物与其他植物杂交。

公开(公告)号：US08959833B2

公开(公告)日：2015-02-24

申请号：US13680603

申请日：2012-11-19

Applicant: Monsanto Technology LLC

Inventor： Kevin L. Deppermann ,  Michael W. Petersen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather Forbes ,  Bruce Schnicker ,  John Tamulonis

IPC: A01B79/00 ,  A01B79/02 ,  A01C1/00 ,  A01G1/00 ,  A01H3/00 ,  A01H1/04 ,  A01H5/10 ,  C12Q1/68 ,  C12N15/82 ,  A01H1/08 ,  A01H1/02

CPC classification number: A01H1/02 ,  A01H1/04 ,  A01H1/08 ,  A01H5/10 ,  C12N15/8241 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method of evaluating seeds for a desired trait, for example, for facilitating germplasm improvement activities, etc., generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, analyzing the tissue samples for one or more characteristics indicative of a desired trait, and selecting the sampled seeds that include the desired trait.

Abstract translation: 本发明提供了通过使用高通量，种子的非破坏性采样来促进种质改良活动的新方法。 评估所需性状的种子的方法，例如用于促进种质改良活动等的方法通常包括使用自动种子取样器从个体种子中除去组织样品，而不影响种子的发芽活力，分析组织样品中的一种或 指示所需性状的更多特征，以及选择包含所需性状的采样种子。

公开(公告)号：US20130167257A1

公开(公告)日：2013-06-27

申请号：US13680603

申请日：2012-11-19

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Kevin L. Deppermann ,  Michael W. Petersen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather Forbes ,  Bruce Schnicker ,  John Tamulonis

IPC: A01H1/04

CPC classification number: A01H1/02 ,  A01H1/04 ,  A01H1/08 ,  A01H5/10 ,  C12N15/8241 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method of evaluating seeds for a desired trait, for example, for facilitating germplasm improvement activities, etc., generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, analyzing the tissue samples for one or more characteristics indicative of a desired trait, and selecting the sampled seeds that include the desired trait.

Abstract translation: 本发明提供了通过使用高通量，种子的非破坏性采样来促进种质改良活动的新方法。 评估所需性状的种子的方法，例如用于促进种质改良活动等的方法通常包括使用自动种子取样器从个体种子中除去组织样品，而不影响种子的发芽活力，分析组织样品中的一种或 指示期望特征的更多特征，以及选择包含期望特征的采样种子。