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1.发明授权Nucleic acids encoding factor X analogues having a modified protease cleavage site 有权编码具有修饰的蛋白酶切割位点的因子X类似物的核酸公开(公告)号：US07220569B2
公开(公告)日：2007-05-22
申请号：US10407123
申请日：2003-04-04
CPC分类号： C12N9/6432 , A61K38/00 , C12Y304/21006
摘要： Nucleic acids encoding factor X analogues are provided that have a modification in the region of the natural Factor Xa activation cleavage site. The modification results in a processing site for a protease not naturally cleaving in this region of the Factor X sequence.
摘要翻译： 提供编码因子X类似物的核酸，其在天然因子Xa激活切割位点的区域具有修饰。 该修饰导致在因子X序列的该区域中不自然切割的蛋白酶的加工位点。
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2.发明授权公开(公告)号：US06905846B2
公开(公告)日：2005-06-14
申请号：US10348504
申请日：2003-01-21
申请人： Michele Himmelspach , Michael Pfleiderer , Falko-Guenter Falkner , Johann Eibl , Friedrich Dorner , Uwe Schlokat
发明人： Michele Himmelspach , Michael Pfleiderer , Falko-Guenter Falkner , Johann Eibl , Friedrich Dorner , Uwe Schlokat
IPC分类号： C12N15/09 , A61K38/00 , A61K38/36 , A61K38/46 , A61K38/48 , A61P7/00 , A61P7/04 , C07K14/475 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/64 , C12N15/57 , C12P21/02 , C12P21/06
CPC分类号： C12N9/6432 , A61K38/00 , C12Y304/21006
摘要： Nucleic acids encoding factor XΔ analogues having a deletion of amino acids Arg180 to Arg234 and a modification in the region of the amino acid sequence between Gly173 and Arg 179 are provided.
摘要翻译： 提供编码具有氨基酸Arg180至Arg234缺失的因子XDelta类似物的核酸，以及Gly173和Arg179之间的氨基酸序列区域的修饰。
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公开(公告)号：US06958322B1
公开(公告)日：2005-10-25
申请号：US09632722
申请日：2000-08-04
申请人： Michele Himmelspach , Uwe Schlokat
发明人： Michele Himmelspach , Uwe Schlokat
CPC分类号： C12N9/6432 , C12Y304/21006
摘要： This invention describes a factor Xa analog which has a substitution of a minimum of one of the amino acid between Glu226 and Arg234 and possibly Ile235, relative to the amino acid numbering according to FIG. 1, a preparation containing the activated form of the factor X analog, and a method for the production of these molecules.
摘要翻译： 本发明描述了相对于根据图1的氨基酸编号，具有至少一个氨基酸在Glu226和Arg234之间以及可能的Ile235的取代的因子Xa类似物。 1，含有因子X类似物的活化形式的制剂，以及这些分子的制备方法。
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公开(公告)号：US06562598B1
公开(公告)日：2003-05-13
申请号：US09367777
申请日：1999-11-18
申请人： Michele Himmelspach , Michael Pfleiderer , Falko-Guenter Falkner , Johann Eibl , Friedrich Dorner , Uwe Schlokat
发明人： Michele Himmelspach , Michael Pfleiderer , Falko-Guenter Falkner , Johann Eibl , Friedrich Dorner , Uwe Schlokat
IPC分类号： C12P2106
CPC分类号： C12N9/6432 , A61K38/00 , C12Y304/21006
摘要： Factor X&Dgr; analogues are provided, as well as pharmaceutical preparations containing such analogues and methods of preparing such analogues. The factor X&Dgr; analogues have a deletion of the amino acids Arg180 to Arg234 and a modification in the region of the amino acid sequence between Gly173 and Arg179 of the factor X amino acid sequence. Such analogues can include a processing site not normally present in factor X, thus allowing for selective conversion of the analogue to an active form. The analogues and preparations have utility in the treatment of a number of blood coagulation disorders.
摘要翻译： 提供因子XDELTA类似物，以及含有此类类似物的药物制剂和制备此类类似物的方法。 因子XDELTA类似物具有氨基酸Arg180至Arg234的缺失和在因子X氨基酸序列的Gly173和Arg179之间的氨基酸序列区域的修饰。 这样的类似物可以包括通常不存在于因子X中的处理位点，从而允许将类似物选择性转化为活性形式。 类似物和制剂可用于治疗许多凝血障碍。
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公开(公告)号：US06573071B1
公开(公告)日：2003-06-03
申请号：US09367791
申请日：1999-11-12
IPC分类号： C12P2100
CPC分类号： C12N9/6432 , A61K38/00 , C12Y304/21006
摘要： Factor X analogues having a modification in the region of the natural Factor Xa activation cleavage site, said modification representing a processing site of a protease not naturally cleaving in this region of the Factor X sequence, preparations containing the Factor X analogues according to the invention, and processes for the preparation thereof are described.
摘要翻译： 因子X类似物在天然因子Xa活化切割位点的区域具有修饰，所述修饰代表在因子X序列的该区域中不自然切割的蛋白酶的加工位点，所述制剂含有本发明的因子X类似物， 并描述其制备方法。
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公开(公告)号：US07575897B2
公开(公告)日：2009-08-18
申请号：US12058473
申请日：2008-03-28
IPC分类号： C12P21/06
CPC分类号： C12N9/644 , A61K38/36 , C12Y304/21022
摘要： The present invention relates to a purified recombinant blood coagulation factor IX (rFIX) preparation, wherein at least 25% of the rFIX in the preparation is fully phosphorylated and sulfated, a cell culture expressing a rFIX resulting in said preparation, a pharmaceutical composition for treating a bleeding disorder comprising said preparation, and a method for treating a bleeding disorder comprising the step of administering said preparation to a patient in need thereof.
摘要翻译： 本发明涉及纯化的重组凝血因子IX（rFIX）制剂，其中至少25％的制剂中的rFIX被完全磷酸化和硫酸化，表达产生所述制备的rFIX的细胞培养物，用于治疗的药物组合物 包括所述制剂的出血病症和治疗出血性疾病的方法，包括将所述制剂给予需要的患者的步骤。
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公开(公告)号：US20080176287A1
公开(公告)日：2008-07-24
申请号：US12058473
申请日：2008-03-28
IPC分类号： C12P21/04
CPC分类号： C12N9/644 , A61K38/36 , C12Y304/21022
摘要： The present invention relates to a purified recombinant blood coagulation factor IX (rFIX) preparation, wherein at least 25% of the rFIX in the preparation is fully phosphorylated and sulfated, a cell culture expressing a rFIX resulting in said preparation, a pharmaceutical composition for treating a bleeding disorder comprising said preparation, and a method for treating a bleeding disorder comprising the step of administering said preparation to a patient in need thereof.
摘要翻译： 本发明涉及纯化的重组凝血因子IX（rFIX）制剂，其中至少25％的制剂中的rFIX被完全磷酸化和硫酸化，表达产生所述制备的rFIX的细胞培养物，用于治疗的药物组合物 包括所述制剂的出血病症和治疗出血性疾病的方法，包括将所述制剂给予需要的患者的步骤。
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公开(公告)号：US20070244036A1
公开(公告)日：2007-10-18
申请号：US11683362
申请日：2007-03-07
CPC分类号： C12N9/644 , A61K38/36 , C12Y304/21022
摘要： The present invention relates to a purified recombinant blood coagulation factor IX (rFIX) preparation, wherein at least 25% of the rFIX in the preparation is fully phosphorylated and sulfated, a cell culture expressing a rFIX resulting in said preparation, a pharmaceutical composition for treating a bleeding disorder comprising said preparation, and a method for treating a bleeding disorder comprising the step of administering said preparation to a patient in need thereof.
摘要翻译： 本发明涉及纯化的重组凝血因子IX（rFIX）制剂，其中至少25％的制剂中的rFIX被完全磷酸化和硫酸化，表达产生所述制备的rFIX的细胞培养物，用于治疗的药物组合物 包括所述制剂的出血病症和治疗出血性疾病的方法，包括将所述制剂给予需要的患者的步骤。
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公开(公告)号：US07375084B2
公开(公告)日：2008-05-20
申请号：US11683362
申请日：2007-03-07
CPC分类号： C12N9/644 , A61K38/36 , C12Y304/21022
摘要： The present invention relates to a purified recombinant blood coagulation factor IX (rFIX) preparation, wherein at least 25% of the rFIX in the preparation is fully phosphorylated and sulfated, a cell culture expressing a rFIX resulting in said preparation, a pharmaceutical composition for treating a bleeding disorder comprising said preparation, and a method for treating a bleeding disorder comprising the step of administering said preparation to a patient in need thereof.
摘要翻译： 本发明涉及纯化的重组凝血因子IX（rFIX）制剂，其中至少25％的制剂中的rFIX被完全磷酸化和硫酸化，表达产生所述制备的rFIX的细胞培养物，用于治疗的药物组合物 包括所述制剂的出血病症和治疗出血性疾病的方法，包括将所述制剂给予需要的患者的步骤。
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公开(公告)号：US5858658A
公开(公告)日：1999-01-12
申请号：US533967
申请日：1995-09-26
申请人： Thomas Haemmerle , Falko-Guenter Falkner , Johann Kohl , Michele Himmelspach , Friedrich Dorner
发明人： Thomas Haemmerle , Falko-Guenter Falkner , Johann Kohl , Michele Himmelspach , Friedrich Dorner
CPC分类号： C12Q1/6876 , C12Q1/6851
摘要： A method of quantitating genomic DNA in a sample is provided. The method comprises the steps of adding to the sample a given amount of at least one nucleic acid as an internal standard, wherein the standard nucleic acid differs from the genomic DNA to be quantified in at least one detectable characteristic; amplifying the genomic DNA and the internal standard nucleic acid by means of a nucleic acid amplification process employing primers complementary to repetitive genomic sequences; determining as a first amount the amount of amplified genomic DNA, and determining as a second amount the amount of amplified standard nucleic acid; and determining from the first and second amounts, as a third amount, the amount of genomic DNA originally contained in the sample. Kits for performing the method and products substantially free of foreign DNA as determined by the method also are provided.
摘要翻译： 提供了一种定量样品中基因组DNA的方法。 该方法包括以下步骤：向样品中加入给定量的至少一种作为内标的核酸，其中标准核酸与待定量的至少一种可检测特征的基因组DNA不同; 通过使用与重复基因组序列互补的引物的核酸扩增方法扩增基因组DNA和内标核酸; 确定扩增的基因组DNA的量作为第一量，并将第二量确定为扩增的标准核酸的量; 并且从第一和第二量确定作为第三量，最初包含在样品中的基因组DNA的量。 还提供了通过该方法确定的用于执行基本上不含外来DNA的方法和产物的试剂盒。
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