公开(公告)号：US20170037483A1

公开(公告)日：2017-02-09

申请号：US15298723

申请日：2016-10-20

Applicant: OLYMPUS CORPORATION

Inventor： Taro HAYASHI ,  Isao SAKANE ,  Yoko OHASHI

IPC: C12Q1/68 ,  G01N21/64 ,  G06K9/00 ,  G06T7/00 ,  G06T7/20 ,  G01N33/483 ,  C12N5/077

CPC classification number: C12Q1/6897 ,  C12N5/0657 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2510/00 ,  G01N21/6486 ,  G01N33/4833 ,  G06K9/00134 ,  G06T7/0012 ,  G06T7/20 ,  G06T7/97 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30048

Abstract: A method for monitoring differentiation into cardiac muscle cells includes keeping, in an alive state, cells into which a reporter gene of luminescent protein configured to vary in luminescence intensity according to an expression of myocardial differentiation marker gene is introduced. The method includes acquiring a luminescence image as a still image by imaging light emitted from the cells in a light shielding state. The method includes acquiring sequential images with illuminating the cells. The method includes associating biological information obtained from the sequential images with biological information obtained from the still image.

Abstract translation: 用于监测心肌细胞分化的方法包括在活体状态下保持根据心肌分化标记基因的表达配置成发光强度变化的发光蛋白质的报道基因的细胞。 该方法包括通过在遮光状态下成像从单元发射的光来获取作为静止图像的发光图像。 该方法包括通过照亮单元获取顺序图像。 该方法包括将从顺序图像获得的生物信息与从静止图像获得的生物信息相关联。

公开(公告)号：US20170315049A1

公开(公告)日：2017-11-02

申请号：US15647486

申请日：2017-07-12

Applicant: OLYMPUS CORPORATION

Inventor： Taro HAYASHI ,  Yoko OHASHI

IPC: G01N15/14 ,  G01N33/483 ,  G03B13/34 ,  H04N5/265 ,  H04N5/232 ,  G01N21/76 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1475 ,  G01N15/1434 ,  G01N21/763 ,  G01N33/4833 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1445 ,  G01N2015/1452 ,  G03B13/34 ,  H04N5/23212 ,  H04N5/265

Abstract: A method for generating a three-dimensional luminescence image includes setting a focal interval between two-dimensional images in accordance with localization of luminescence in a three-dimensional sample. The three-dimensional sample contains a plurality of cells prepared to be luminescent and has a three-dimensional shape. The two-dimensional images have mutually different focal planes and are acquired at the focal interval. The method further includes acquiring a two-dimensional image set including two-dimensional images at the focal interval that is set by imaging the three-dimensional sample under an unirradiated condition; and generating a three-dimensional luminescence image by combining the two-dimensional images included in the two-dimensional image set together.