公开(公告)号：US20160377605A1

公开(公告)日：2016-12-29

申请号：US15194188

申请日：2016-06-27

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Kyo Han AHN ,  Yong Woong JUN ,  Donghee MA

IPC: G01N33/53 ,  G01N33/58 ,  G01N21/64 ,  C07F5/02

CPC classification number: G01N33/5308 ,  C07F5/022 ,  C12Q1/008 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5735 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2400/00

Abstract: A fluorescent probe compound, a preparation method thereof, and a method of imaging and quantifying lysosomal adenosine triphosphate (ATP) at a cell or tissue level through one-photon or two-photon fluorescence microscopy using the compound is disclosed. A fluorescence detection system capable of detecting lysosomal ATP is further disclosed. Since the fluorescent probe compound is found to be capable of selectively sensing and quantifying lysosomal ATP in a cell or tissue, it is expected that the disclosed compound or composition can be usefully employed in the study of various biological reactions or diseases associated with ATP in a living body.

Abstract translation: 公开了一种荧光探针化合物及其制备方法，以及通过使用该化合物的单光子或双光子荧光显微镜在细胞或组织水平上成像和定量溶酶体三磷酸腺苷（ATP）的方法。 进一步公开了能够检测溶酶体ATP的荧光检测系统。 由于发现荧光探针化合物能够选择性地感测和定量细胞或组织中的溶酶体ATP，所以预期所公开的化合物或组合物可有效地用于研究各种生物反应或与ATP相关的疾病 活体。

公开(公告)号：US20160202263A1

公开(公告)日：2016-07-14

申请号：US14745973

申请日：2015-06-22

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Kyo Han AHN ,  Hyunsoo MOON ,  Dokyoung KIM ,  Ki Hean KIM ,  Hye Gun RYU

IPC: G01N33/58 ,  G01N21/64 ,  C07D311/92 ,  G01N33/68 ,  C07D311/78 ,  C07D311/94

CPC classification number: G01N33/582 ,  A61K49/0021 ,  C07D311/78 ,  C07D311/92 ,  C07D311/94 ,  G01N21/6428 ,  G01N33/6896 ,  G01N2021/6439 ,  G01N2333/4709 ,  G01N2800/2821

Abstract: Provided are acedan derivatives having an extended π bond, a method for preparing the acedan derivatives, and a method for two-photon microscopy imaging of amyloid-beta plaque using the acedan derivatives; more particularly, to two-photon absorbing fluorescent compounds having a longer absorption wavelength and emission wavelength than acedan and acedan derivatives which are conventional two-photon absorbing fluorophores. The compounds provided may be usefully used for in vivo imaging studies by imaging cells or tissue using the compounds, and may also be usefully used for diagnosing Alzheimer's disease by imaging amyloid-beta plaque using the compounds.

Abstract translation: 提供的是具有延伸＆ 键，一种制备acedan衍生物的方法，以及使用acedan衍生物的淀粉样蛋白-β斑块双光子显微镜成像方法。 更具体地说，涉及具有比作为常规双光子吸收荧光团的常规阿敏和acedan衍生物更长的吸收波长和发射波长的双光子吸收荧光化合物。 所提供的化合物可以有用地用于通过使用化合物成像细胞或组织的体内成像研究，并且还可以有用地用于通过使用该化合物成像淀粉样蛋白-β斑块来诊断阿尔茨海默氏病。

公开(公告)号：US20200247831A1

公开(公告)日：2020-08-06

申请号：US16642439

申请日：2018-08-31

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Kyo Han AHN ,  Hye Gun RYU ,  Kyeong Hwan KIM ,  Yong Woong JUN ,  Subhankar SINGHA

IPC: C07F7/08 ,  A61K49/00

Abstract: The present invention relates to a silicon-substituted amino-pyronin compound derivative, a cell- or tissue-imaging method using the same, a cancer diagnosis method using the same, and a method for preparation of the same. More specifically, serving as a two-photon absorption fluorescent substance which has longer absorption and emission wavelengths in red or near-infrared regions of 625 nm or greater, compared to conventional one-photon absorption fluorescent substances, the silicon-substituted amino-pyronin compound derivative can minimize the influence of self-fluorescence in a bioimaging study and is thus expected to be suitable for high-resolution imaging in deep tissues. A fluorescent probe developed on the basis of the fluorescent substance platform is expected to find an expanded range of applications in analyzing and imaging specific materials in vivo.

公开(公告)号：US20170327509A1

公开(公告)日：2017-11-16

申请号：US15512624

申请日：2015-05-06

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Kyo Han AHN ,  Hyunsoo MOON ,  Dokyoung KIM ,  Subhankar SINGHA ,  Basab ROY ,  Sunderraman SAMBASIVAN

IPC: C07D487/08 ,  C07D221/14 ,  C07D207/08 ,  C07C231/08 ,  C07C221/00 ,  A61K49/00 ,  C07D295/116 ,  C07C303/38 ,  C07C2601/14

CPC classification number: C07D487/08 ,  A61K49/0021 ,  C07C221/00 ,  C07C225/22 ,  C07C231/08 ,  C07C233/33 ,  C07C303/38 ,  C07C311/20 ,  C07C2601/14 ,  C07D207/08 ,  C07D221/14 ,  C07D295/116

Abstract: The present invention relates to new one-photon or two-photon absorbing fluorophores, a method for preparing the same, and a method for cellular imaging using the same, and more particularly, to new two-photon absorbing fluorophores having higher fluorescence quantum yield and two-photon absorption cross-section value than those of the conventional two-photon absorbing fluorophore, acedan, and thus are promisingly applicable in bioimaging. The design strategy and the compounds according to the present invention may practically utilized for developing new D-π-A fluorophores.

公开(公告)号：US20160116455A1

公开(公告)日：2016-04-28

申请号：US14888883

申请日：2014-03-10

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Kyo Han AHN ,  Dokyoung KIM ,  Sungjee KIM ,  Ki Hean KIM

IPC: G01N33/50 ,  C07C223/06

CPC classification number: G01N33/5023 ,  C07C223/06 ,  C09K11/06 ,  C12Q1/485 ,  G01N33/574 ,  G01N33/582 ,  G01N2333/91205 ,  G01N2333/9121 ,  G01N2500/10

Abstract: A fluorescent probe for detecting a tyrosine kinase using a compound having an ortho-hydroxy-benzaldehyde structure, and use thereof are provided. The fluorescent probe can show a change in fluorescence when the compound binds with a tyrosine kinase. The compound can be readily synthesized and has high stability and low cytotoxicity in vivo. The fluorescent probe can be used to image cells or tissues overexpressing the tyrosine kinase, the fluorescent probe can be effectively used in a composition for imaging the tissues and a method of imaging the tissues. Also, the fluorescent probe can be used to image cancer cells or tissues since the fluorescent probe can exhibit fluorescence when the fluorescent probe binds to the cancer cells or tissues overexpressing the tyrosine kinase.

Abstract translation: 提供了使用具有邻羟基 - 苯甲醛结构的化合物检测酪氨酸激酶的荧光探针及其用途。 当化合物与酪氨酸激酶结合时，荧光探针可以显示荧光的变化。 该化合物可以容易地合成并且在体内具有高稳定性和低细胞毒性。 荧光探针可用于对过量表达酪氨酸激酶的细胞或组织进行成像，荧光探针可以有效地用于组织中用于成像组织和使组织成像的方法。 此外，荧光探针可以用于对癌细胞或组织进行成像，因为当荧光探针结合到癌细胞或过表达酪氨酸激酶的组织时，荧光探针可以显示出荧光。