公开(公告)号：US20180066231A1

公开(公告)日：2018-03-08

申请号：US15553868

申请日：2016-02-26

Applicant: ROHTO PHARMACEUTICAL CO., LTD.

Inventor： Yoshifumi IKEYAMA ,  Toru OKUBO ,  Hiroyuki NISHIDA ,  Tomohiro TSUDA ,  Eiko UNO ,  Masayo YUMOTO

IPC: C12N5/0775

CPC classification number: C12N5/0667 ,  C12N2500/05 ,  C12N2500/30 ,  C12N2501/10 ,  C12N2501/39

Abstract: The present invention aims to provide a mesenchymal stem cell culture medium suitable for culturing umbilical cord mesenchymal stem cells in particular, among the mesenchymal stem cells.The present invention is of a mesenchymal stem cell culture medium comprising at least 2 kinds of components selected from the group consisting of a PTEN inhibitor, a p53 inhibitor, a p38 inhibitor, an Wnt signal activator and a ROCK inhibitor as well as basal medium for culturing animal cells.

公开(公告)号：US20210046122A1

公开(公告)日：2021-02-18

申请号：US16961354

申请日：2019-01-11

Applicant: OSAKA UNIVERSITY ,  ROHTO PHARMACEUTICAL CO., LTD.

Inventor： Kohji NISHIDA ,  Ryuhei HAYASHI ,  Shun SHIBATA ,  Toru OKUBO ,  Yoichi HONMA

IPC: A61K35/28 ,  A61P27/02

Abstract: The present invention addresses the problem of providing a novel therapeutic agent that is effective against diseases involving stratified squamous epithelial cells such as dry eye syndrome. The present invention is an agent for promoting normal differentiation/maturation of stratified squamous epithelial cells, which comprises a secretion of mesenchymal stem cells. It is preferable that the secretions not include animal serum, that the mesenchymal stem cells be adipose-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or bone marrow-derived mesenchymal stem cells, and that the stratified squamous epithelial cells be at least one selected from the group consisting of corneal epithelial cells, conjunctival epithelial cells, epidermal keratinocytes, oral epithelial cells, epiglottic epithelial cells, esophageal epithelial cells, vaginal epithelial cells, vocal fold epithelial cells, nasal epithelial cells, and nasal vestibular epithelial cells.

公开(公告)号：US20190015452A1

公开(公告)日：2019-01-17

申请号：US15749766

申请日：2016-08-03

Applicant: OSAKA UNIVERSITY ,  ROHTO PHARMACEUTICAL CO., LTD.

Inventor： Kohji NISHIDA ,  Ryuuhei HAYASHI ,  Yoichi HONMA ,  Toru OKUBO ,  Shun SHIBATA

IPC: A61K35/28 ,  A61P27/02 ,  C12N5/0775 ,  C12N5/00

Abstract: The present invention relates to mesenchymal stem cell-derived microparticles having activity that promotes the growth of corneal epithelial stem cells and/or corneal epithelial cells, activity that maintains corneal epithelial stem cells in an undifferentiated state or promotes the formation of colonies thereby, and function that protects corneal epithelium.