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公开(公告)号：US4618584A
公开(公告)日：1986-10-21
申请号：US684205
申请日：1984-12-20
IPC分类号： C12N9/92
CPC分类号： C12N9/92 , B01D61/145 , B01D2313/42 , Y10S435/814
摘要： This invention relates to a process for the production of a purified glucose isomerase enzyme which comprises contacting an enzyme extract containing glucose isomerase and impurities with a first polysulfone membrane not normally permeable to glucose isomerase, in the presence of a salt concentration capable of selectively inducing permeation of glucose isomerase through the membrane, and obtaining a glucose isomerase containing permeate.
摘要翻译： 本发明涉及一种生产纯化葡萄糖异构酶的方法，该方法包括在含有能够选择性诱导渗透的盐浓度的情况下使含有葡萄糖异构酶和杂质的酶提取物与葡萄糖异构酶通常不能通过的第一聚砜膜接触 的葡萄糖异构酶通过膜，并获得含葡萄糖异构酶的渗透物。
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公开(公告)号：US4663288A
公开(公告)日：1987-05-05
申请号：US736921
申请日：1985-05-22
CPC分类号： C12N9/92 , C12N9/00 , Y10S435/814
摘要： The disclosure is directed to the resolubilization of an insoluble glucose isomerase-amine complex, wherein the amine has the general formula: ##STR1## The insoluble enzyme complex may be resolubilized to produce a stable concentrated and purified glucose isomerase preparation by reaction with a resolubilizing mixture comprising a cation exchange resin.
摘要翻译： 本发明涉及不溶性葡萄糖异构酶 - 胺络合物的再溶解，其中胺具有以下通式：不溶性酶复合物可以通过与再溶解混合物反应而再溶解以产生稳定的浓缩和纯化的葡萄糖异构酶制剂 包括阳离子交换树脂。
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公开(公告)号：US4610965A
公开(公告)日：1986-09-09
申请号：US684204
申请日：1984-12-20
IPC分类号： C12N9/92
CPC分类号： C12N9/92 , Y10S435/815
摘要： The present invention relates to a process for the production of a purified glucose isomerase which comprises contacting an impure extract containing glucose isomerase and soluble impurities with a weakly basic ion exchange material known to adsorb glucose isomerase; adding a first salt solution of a concentration which removes unadsorbed and weakly adsorbed impurities, but not glucose isomerase; and adding a second, buffered salt solution which elutes the adsorbed glucose isomerase.
摘要翻译： 本发明涉及一种生产纯化葡萄糖异构酶的方法，该方法包括将含有葡萄糖异构酶和可溶性杂质的不纯提取物与已知吸附葡萄糖异构酶的弱碱性离子交换材料接触; 加入一种浓度的第一盐溶液，除去未吸附和弱吸附的杂质，而不是葡萄糖异构酶; 并加入第二缓冲盐溶液洗脱吸附的葡萄糖异构酶。
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4.发明授权Method for maintaining immobilized glucose isomerase activity during continuous isomerization of glucose to fructose 失效在将葡萄糖连续异构化成果糖期间维持固定的葡萄糖异构酶活性的方法
公开(公告)号：US5177005A
公开(公告)日：1993-01-05
申请号：US762034
申请日：1991-09-16
申请人： Norman E. Lloyd , Richard L. Antrim
发明人： Norman E. Lloyd , Richard L. Antrim
摘要： This invention provides a method for the maintenance of continuous activity of an immobilized enzyme reaction by initially underloading an adsorbent with a desired enzyme, monitoring the activity of the enzyme and periodically adding fresh enzyme to maintain a constant, continuous level of activity until the maximum carrying capacity of the support is reached. The method is preferably carried out when continuously isomerizing glucose to fructose with glucose isomerase adsorbed to a weakly basic anion exchange resin.
摘要翻译： 本发明提供了一种维持固定化酶反应的连续活性的方法，其中首先使用所需酶负载吸附剂，监测酶的活性并周期性地加入新鲜酶以保持恒定的连续水平的活性，直到最大携带 达成了支持能力。 该方法优选在葡萄糖与弱碱性阴离子交换树脂吸附的葡萄糖异构酶连续异构化为果糖时进行。
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5.发明授权Oxidoreductase enzyme stabilized highly unsaturated fatty acids and derivatives of such acids 失效氧化还原酶稳定的高度不饱和脂肪酸及其衍生物
公开(公告)号：US4963368A
公开(公告)日：1990-10-16
申请号：US182629
申请日：1988-04-18
CPC分类号： C07C51/50 , A23L3/3571 , A23P10/30 , A61K31/20 , A61K9/5057 , A61K9/5063
摘要： Highly unsaturated fatty acid compounds, and derivatives thereof, are stabilized against oxidation with a water activated oxidoreductase enzyme. The fatty acid containing component is preferably microencapsulated in a wall member which comprises the enzyme.
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公开(公告)号：US4857339A
公开(公告)日：1989-08-15
申请号：US101561
申请日：1987-09-28
CPC分类号： C12Y302/01001 , A23L7/107 , C12P19/24 , C12Y302/01003 , C12Y302/01004 , C12Y503/01005 , C13K1/06 , C13K11/00
摘要： Breakfast cereals are sweetened by treating cereal grains or at least one cereal grain fraction such as bran, with enzymes comprising glucoamylase and glucose isomerase to produce fructose while retaining cereal particle discreteness or integrity. Enzymatic treatment with alpha-amylase may be initiated prior to, during, or after cooking. The enzymatically treated, cooked cereal grains are formed into breakfast cereal shapes and the enzymes are inactivated to provide a shelf-stable cereal product. The cereal products exhibit a sweet, pleasing complex-honey-like taste and aroma. Producing fructose provides a greater level of sweetness for a given amount of starch conversion into low molecular weight reducing sugars such as mono- and di-saccharides. In achieving a given level of sweetness, more starch or high molecular weight dextrins may be retained for their matrix forming ability or for improved machineability of the enzymatically treated cereal grains into breakfast cereal shapes. The naturally sweetened cereal products of the present invention may be in shredded, flaked, ground, or extruded form.
摘要翻译： 早餐谷物通过处理谷物或至少一种谷物谷物部分如麸皮，通过酶包含葡糖淀粉酶和葡萄糖异构酶来甜化以产生果糖，同时保持谷物颗粒离散性或完整性。 用α-淀粉酶进行酶法处理可以在烹饪之前，期间或之后开始。 酶处理的熟熟谷物形成早餐谷物形状，酶被灭活以提供货架稳定的谷物产品。 谷物产品呈现甜美，复杂的蜂蜜样味道和香气。 生产果糖为给定量的淀粉转化成低分子量还原糖如单糖和二糖提供了更高水平的甜度。 为了达到一定水平的甜度，可以保留更多的淀粉或高分子量糊精用于其基质形成能力，或者将酶处理过的谷物的可加工性提高为早餐谷物形状。 本发明的天然甜味谷物产品可以是切碎的，片状的，研磨的或挤出的形式。
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公开(公告)号：US07405293B1
公开(公告)日：2008-07-29
申请号：US10653037
申请日：2003-08-29
IPC分类号： C08B30/12
CPC分类号： C08B30/18
摘要： Disclosed are a method for the reduction of an oligosaccharide mixture and an oligosaccharide mixture prepared thereby. In accordance with the disclosed invention, a mixture of oligosaccharides having a given DP profile is reduced to a DE of essentially zero by catalytically hydrogenating the mixture under reaction conditions sufficient to preserve the DP profile of the mixture, which reaction conditions typically include a reaction temperature ranging from about 50° C. to about 150° C. and a reaction pressure ranging up to about 1500 psi. Surprisingly, when the mixture is a malto-oligosaccharide mixture, the reduced mixture will have a superior color-fastness and thermal stability as compared to a similar unreduced mixture of malto-oligosaccharides, and also low reactivity towards nitrogen-containing species.
摘要翻译： 公开了由此制备的寡糖混合物和寡糖混合物的还原方法。 根据所公开的发明，具有给定DP分布的寡糖的混合物通过在足以保持混合物的DP分布的反应条件下催化氢化混合物而还原为DE，该反应条件通常包括反应温度 范围为约50℃至约150℃，反应压力范围高达约1500psi。 令人吃惊的是，当混合物是麦芽低聚糖混合物时，与类似的未还原的麦芽寡糖混合物相比，还原的混合物具有优异的耐色牢度和热稳定性，并且对含氮物质的反应性低。
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8.发明授权Animal litter, process for preparing animal litter, and method for removal of animal waste 有权动物垃圾，动物垃圾的制备方法和动物废物的清除方法公开(公告)号：US06405677B2
公开(公告)日：2002-06-18
申请号：US09725982
申请日：2000-11-29
IPC分类号： A01K2900
CPC分类号： A01K1/0155
摘要： An animal litter that includes seed meal. The animal litter comprises discrete plural particles of a seed meal, which particles tend to agglomerate into clumps when wetted. The litter of the invention absorbs liquids such as animal excretions, and substantially reduces the odor thereof. Preferably, the litter further includes a grain germ, such as corn germ.
摘要翻译： 包括种子粉的动物垃圾。 动物垃圾包括离散的多个颗粒的颗粒，当润湿时该颗粒倾向于聚集成团块。 本发明的垃圾吸收诸如动物排泄物的液体，并且基本上减少其气味。 优选地，垃圾还包括谷物胚芽，例如玉米胚芽。
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9.发明授权Liquefaction of granular starch slurries using an antioxidant with alpha amylase 失效使用具有α淀粉酶的抗氧化剂的颗粒状淀粉浆的液化
公开(公告)号：US5322778A
公开(公告)日：1994-06-21
申请号：US785624
申请日：1991-10-31
申请人： Richard L. Antrim , Leif P. Solheim
发明人： Richard L. Antrim , Leif P. Solheim
CPC分类号： C12P19/14
摘要： The present invention relates to a process of liquefying starch wherein the liquefaction step is carried out at pH less than 6. It also relates to the use of antioxidants.
摘要翻译： 本发明涉及一种液化淀粉的方法，其中液化步骤在pH小于6的条件下进行。它还涉及使用抗氧化剂。
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公开(公告)号：US06919446B1
公开(公告)日：2005-07-19
申请号：US09614961
申请日：2000-07-13
IPC分类号： C07H3/06 , C07B61/00 , C07H1/00 , C07H15/04 , C08B30/18 , C08B31/00 , C08B37/00 , C07G17/00 , C13K7/00
摘要： Disclosed are a method for the reduction of an oligosaccharide mixture and an oligosaccharide mixture prepared thereby. In accordance with the disclosed invention, a mixture of oligosaccharides having a given DP profile is reduced to a DE of essentially zero by catalytically hydrogenating the mixture under reaction conditions sufficient to preserve the DP profile of the mixture, which reaction conditions typically include a reaction temperature ranging from about 50° C. to about 150° C. and a reaction pressure of at least about 1500 psi. Surprisingly, when the mixture is a malto-oligosaccharide mixture, the reduced mixture will have a superior color-fastness and thermal stability as compared to a similar unreduced mixture of malto-oligosaccharides, and also low reactivity towards nitrogen-containing species.
摘要翻译： 公开了由此制备的寡糖混合物和寡糖混合物的还原方法。 根据所公开的发明，具有给定DP分布的寡糖的混合物通过在足以保持混合物的DP分布的反应条件下催化氢化混合物而还原为DE，该反应条件通常包括反应温度 范围为约50℃至约150℃，反应压力为至少约1500psi。 令人吃惊的是，当混合物是麦芽低聚糖混合物时，与类似的未还原的麦芽寡糖混合物相比，还原的混合物具有优异的耐色牢度和热稳定性，并且对含氮物质的反应性低。
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