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公开(公告)号：US4618584A
公开(公告)日：1986-10-21
申请号：US684205
申请日：1984-12-20
IPC分类号： C12N9/92
CPC分类号： C12N9/92 , B01D61/145 , B01D2313/42 , Y10S435/814
摘要： This invention relates to a process for the production of a purified glucose isomerase enzyme which comprises contacting an enzyme extract containing glucose isomerase and impurities with a first polysulfone membrane not normally permeable to glucose isomerase, in the presence of a salt concentration capable of selectively inducing permeation of glucose isomerase through the membrane, and obtaining a glucose isomerase containing permeate.
摘要翻译： 本发明涉及一种生产纯化葡萄糖异构酶的方法，该方法包括在含有能够选择性诱导渗透的盐浓度的情况下使含有葡萄糖异构酶和杂质的酶提取物与葡萄糖异构酶通常不能通过的第一聚砜膜接触 的葡萄糖异构酶通过膜，并获得含葡萄糖异构酶的渗透物。
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公开(公告)号：US4663288A
公开(公告)日：1987-05-05
申请号：US736921
申请日：1985-05-22
CPC分类号： C12N9/92 , C12N9/00 , Y10S435/814
摘要： The disclosure is directed to the resolubilization of an insoluble glucose isomerase-amine complex, wherein the amine has the general formula: ##STR1## The insoluble enzyme complex may be resolubilized to produce a stable concentrated and purified glucose isomerase preparation by reaction with a resolubilizing mixture comprising a cation exchange resin.
摘要翻译： 本发明涉及不溶性葡萄糖异构酶 - 胺络合物的再溶解，其中胺具有以下通式：不溶性酶复合物可以通过与再溶解混合物反应而再溶解以产生稳定的浓缩和纯化的葡萄糖异构酶制剂 包括阳离子交换树脂。
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公开(公告)号：US4610965A
公开(公告)日：1986-09-09
申请号：US684204
申请日：1984-12-20
IPC分类号： C12N9/92
CPC分类号： C12N9/92 , Y10S435/815
摘要： The present invention relates to a process for the production of a purified glucose isomerase which comprises contacting an impure extract containing glucose isomerase and soluble impurities with a weakly basic ion exchange material known to adsorb glucose isomerase; adding a first salt solution of a concentration which removes unadsorbed and weakly adsorbed impurities, but not glucose isomerase; and adding a second, buffered salt solution which elutes the adsorbed glucose isomerase.
摘要翻译： 本发明涉及一种生产纯化葡萄糖异构酶的方法，该方法包括将含有葡萄糖异构酶和可溶性杂质的不纯提取物与已知吸附葡萄糖异构酶的弱碱性离子交换材料接触; 加入一种浓度的第一盐溶液，除去未吸附和弱吸附的杂质，而不是葡萄糖异构酶; 并加入第二缓冲盐溶液洗脱吸附的葡萄糖异构酶。
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公开(公告)号：US4857339A
公开(公告)日：1989-08-15
申请号：US101561
申请日：1987-09-28
CPC分类号： C12Y302/01001 , A23L7/107 , C12P19/24 , C12Y302/01003 , C12Y302/01004 , C12Y503/01005 , C13K1/06 , C13K11/00
摘要： Breakfast cereals are sweetened by treating cereal grains or at least one cereal grain fraction such as bran, with enzymes comprising glucoamylase and glucose isomerase to produce fructose while retaining cereal particle discreteness or integrity. Enzymatic treatment with alpha-amylase may be initiated prior to, during, or after cooking. The enzymatically treated, cooked cereal grains are formed into breakfast cereal shapes and the enzymes are inactivated to provide a shelf-stable cereal product. The cereal products exhibit a sweet, pleasing complex-honey-like taste and aroma. Producing fructose provides a greater level of sweetness for a given amount of starch conversion into low molecular weight reducing sugars such as mono- and di-saccharides. In achieving a given level of sweetness, more starch or high molecular weight dextrins may be retained for their matrix forming ability or for improved machineability of the enzymatically treated cereal grains into breakfast cereal shapes. The naturally sweetened cereal products of the present invention may be in shredded, flaked, ground, or extruded form.
摘要翻译： 早餐谷物通过处理谷物或至少一种谷物谷物部分如麸皮，通过酶包含葡糖淀粉酶和葡萄糖异构酶来甜化以产生果糖，同时保持谷物颗粒离散性或完整性。 用α-淀粉酶进行酶法处理可以在烹饪之前，期间或之后开始。 酶处理的熟熟谷物形成早餐谷物形状，酶被灭活以提供货架稳定的谷物产品。 谷物产品呈现甜美，复杂的蜂蜜样味道和香气。 生产果糖为给定量的淀粉转化成低分子量还原糖如单糖和二糖提供了更高水平的甜度。 为了达到一定水平的甜度，可以保留更多的淀粉或高分子量糊精用于其基质形成能力，或者将酶处理过的谷物的可加工性提高为早餐谷物形状。 本发明的天然甜味谷物产品可以是切碎的，片状的，研磨的或挤出的形式。
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公开(公告)号：US4011137A
公开(公告)日：1977-03-08
申请号：US500975
申请日：1974-08-26
CPC分类号： C12P19/20 , C12N11/08 , C12N11/10 , C12N11/14 , C12N11/18 , C13K1/06 , Y10S435/813 , Y10S435/839 , Y10S435/918
摘要： Process for converting starch to dextrose wherein a partially hydrolyzed starch solution containing at least 10 percent hydrolyzed starch is contacted with an enzyme system under conditions whereby substantially complete conversion of the starch to dextrose is achieved. The enzyme system comprises immobilized glucoamylase and alpha-amylase selected from the group consisting of soluble alpha-amylase, immobilized alpha-amylase and mixtures thereof.
摘要翻译： 将淀粉转化为葡萄糖的方法，其中将含有至少10％水解淀粉的部分水解淀粉溶液与酶体系接触，在此条件下实现淀粉基本上完全转化为葡萄糖。 酶系统包括固定化葡糖淀粉酶和选自可溶性α-淀粉酶，固定的α-淀粉酶及其混合物的α-淀粉酶。
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公开(公告)号：US4634673A
公开(公告)日：1987-01-06
申请号：US594188
申请日：1984-03-28
CPC分类号： C12N9/92 , Y10S435/814 , Y10S435/816
摘要： The disclosure is directed to the treatment of a glucose isomerase extract with an amine having the general formula: ##STR1## An insoluble complex containing enzyme activity is formed. The insoluble enzyme complex may be resolubilized to produce a stable concentrated and purified glucose isomerase preparation.
摘要翻译： 本公开涉及用具有以下通式的胺处理葡萄糖异构酶提取物：形成含有酶活性的不溶性复合物。 不溶性酶复合物可以重新溶解以产生稳定的浓缩和纯化的葡萄糖异构酶制剂。
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公开(公告)号：US4347322A
公开(公告)日：1982-08-31
申请号：US224590
申请日：1981-01-12
CPC分类号： C12N9/2428 , C12N11/08 , C12N11/12 , C12N9/00 , C12N9/2408 , C12N9/2414 , C12N9/92 , Y10S435/815
摘要： Enzyme purification is carried out by contacting an impure liquid enzyme preparation containing enzyme and soluble impurities with an ion exchange material in a column to adsorb both the enzyme and impurities by the ion exchange material, adding an additional amount of the impure liquid enzyme preparation whereby the soluble impurities therein are preferentially adsorbed by the ion exchange material and the adsorbed enzyme is displaced from the ion exchange material to produce a purified liquid enzyme preparation containing higher enzyme activity than before purification. The purified enzyme is more highly adsorbed by ion exchange material when immobilizing the enzyme.
摘要翻译： 酶纯化通过使含有酶和可溶性杂质的不纯液体酶制剂与柱中的离子交换材料接触，通过离子交换材料吸附酶和杂质，进行附加量的不纯液体酶制剂，从而使 其中的可溶性杂质优先被离子交换材料吸附，并且吸附的酶从离子交换材料移位以产生含有比纯化之前更高的酶活性的纯化液体酶制剂。 当固定酶时，纯化的酶被离子交换材料更高度吸附。
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公开(公告)号：US4102745A
公开(公告)日：1978-07-25
申请号：US773450
申请日：1977-03-02
CPC分类号： C12P19/20 , C12N11/08 , C12N11/10 , C12N11/14 , C12N11/18 , C13K1/06 , Y10S435/813 , Y10S435/839 , Y10S435/918
摘要： Process for converting starch to dextrose wherein a partially hydrolyzed starch solution containing at least 10 percent hydrolyzed starch is contacted with an enzyme system under conditions whereby substantially complete conversion of the starch to dextrose is achieved. The enzyme system comprises immobilized glucoamylase and alpha-amylase selected from the group consisting of soluble alpha-amylase, immobilized alpha-amylase and mixtures thereof.
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公开(公告)号：US09280919B2
公开(公告)日：2016-03-08
申请号：US13526316
申请日：2012-06-18
CPC分类号： G09B29/106 , G06T13/40
摘要： A method of generating anthropomorphic vehicle images for a vehicle owner is shown that includes providing a plurality of anthropomorphic vehicle images on a visual display for a vehicle owner to select; receiving a selection of an anthropomorphic vehicle image from the vehicle owner using a wireless device; obtaining vehicle data using one or more vehicle sensors located on the vehicle; altering at least a portion of the selected anthropomorphic vehicle image based on the accessed vehicle data; and displaying the altered anthropomorphic vehicle image on a wireless device to the vehicle owner.
摘要翻译： 示出了为车主生成拟人车辆图像的方法，其包括在视觉显示器上提供多个人形车辆图像以供车主选择; 使用无线设备从车主接收选择的拟人车辆图像; 使用位于车辆上的一个或多个车辆传感器获取车辆数据; 基于所访问的车辆数据改变所选择的拟人车辆图像的至少一部分; 以及在无线设备上向车主显示改变的拟人车辆图像。
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10.发明授权System for and method of monitoring real time traffic conditions using probe vehicles 有权使用探测车监控实时交通状况的系统和方法公开(公告)号：US08112219B2
公开(公告)日：2012-02-07
申请号：US11272577
申请日：2005-11-11
CPC分类号： G08G1/0104 , G08G1/20
摘要： A system and method for updating and communicating traffic information to at least one receiving vehicle includes a traffic information center and at least one probe vehicle. In a preferred embodiment, major road sections are represented as links, the center stores and updates a parent map database of links that are associable with a first value of a condition, each probe vehicle is configured to receive a copy of the database either periodically or upon request, determine a second value of the condition, and compare the first and second values to determine a condition discrepancy. Where the discrepancy surpasses a threshold, each probe vehicle is further configured to upload at least the second value to the center, so as to update the parent map database.
摘要翻译： 用于向至少一个接收车辆更新和传送交通信息的系统和方法包括交通信息中心和至少一个探测车辆。 在优选实施例中，主要道路部分被表示为链接，中心存储并更新与条件的第一值相关联的链接的父图谱数据库，每个探测车辆被配置为周期性地接收数据库的副本， 根据请求确定条件的第二值，并比较第一和第二值以确定条件差异。 在差异超过阈值的情况下，每个探测车辆还被配置为至少将第二值上传到中心，以便更新父图谱数据库。
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