公开(公告)号：US09700252B2

公开(公告)日：2017-07-11

申请号：US13958404

申请日：2013-08-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Reinhold Mischler ,  Martin Hajnsek ,  Harvey Buck ,  Walter Jernigan

IPC: A61B5/05 ,  A61B5/1486 ,  A61B5/145

CPC classification number: A61B5/14865 ,  A61B5/14532

Abstract: An in vivo amperometric sensor is provided for measuring the concentration of an analyte in a body fluid. The sensor comprises a counter electrode and a working electrode, and the working electrode comprises a sensing layer which is generally water permeable and arranged on a support member adjacent to a contact pad. The sensing layer comprises an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal. The sensing layer has an upper surface facing the body fluid and a lower surface facing away from the body fluid, and the immobilized enzyme is distributed within the sensing layer in such a way that the enzyme concentration in the middle between the upper and lower surfaces is at least as high as on the upper surface of the sensing layer.

公开(公告)号：US20150038814A1

公开(公告)日：2015-02-05

申请号：US13958404

申请日：2013-08-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Reinhold Mischler ,  Martin Hajnsek ,  Harvey Buck ,  Walter Jernigan

IPC: A61B5/1486

CPC classification number: A61B5/14865 ,  A61B5/14532

Abstract: An in vivo amperometric sensor is provided for measuring the concentration of an analyte in a body fluid. The sensor comprises a counter electrode and a working electrode, and the working electrode comprises a sensing layer which is generally water permeable and arranged on a support member adjacent to a contact pad. The sensing layer comprises an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal. The sensing layer has an upper surface facing the body fluid and a lower surface facing away from the body fluid, and the immobilized enzyme is distributed within the sensing layer in such a way that the enzyme concentration in the middle between the upper and lower surfaces is at least as high as on the upper surface of the sensing layer.

公开(公告)号：US20130140176A1

公开(公告)日：2013-06-06

申请号：US13756903

申请日：2013-02-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Terry Beaty ,  Henning Groll ,  Harvey Buck ,  Eric Diebold ,  Abner Joseph ,  Randy Riggles

IPC: G01N27/327

CPC classification number: G01N27/327 ,  G01N27/3272

Abstract: The present invention relates to a biosensor. The biosensor includes a support substrate, electrodes positioned on the support substrate, a spacer substrate positioned on the support substrate, and a cover positioned on the spacer substrate. The cover cooperates with the support substrate to define a capillary channel. The electrodes include at least one working electrode defining a working electrode area in the capillary channel. The working electrode is configured to minimize variation in the working electrode area in the capillary channel due to variations in the spacer substrate placement relative to the working electrode.

Abstract translation: 本发明涉及生物传感器。 生物传感器包括支撑基板，位于支撑基板上的电极，位于支撑基板上的间隔基板和位于间隔基板上的盖。 盖与支撑基板配合以限定毛细通道。 电极包括限定毛细通道中的工作电极区域的至少一个工作电极。 工作电极被配置为使得由于间隔基板相对于工作电极的位置的变化而使毛细通道中的工作电极面积的变化最小化。

公开(公告)号：US20150320345A1

公开(公告)日：2015-11-12

申请号：US14803770

申请日：2015-07-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Harvey Buck ,  Georgeta Lica ,  Karl-Heinz Koelker ,  Ewald Rieger

IPC: A61B5/1477

CPC classification number: A61B5/1477 ,  A61B5/1473 ,  A61B2560/0468 ,  A61B2562/043 ,  G01N27/327

Abstract: The present invention provides an electrochemical sensor that employs multiple electrode areas that are exposed for contact with a body fluid, e.g., when the sensor is inserted subcutaneously into a patient's skin. The exposed electrode areas are arranged symmetrically, such that a symmetrical potential distribution is produced when an AC signal is applied to the sensor. The sensors in accordance with these teachings can advantageously be used with AC signals to determine characteristics of the sensor and thus improve sensor performance. These teachings also provide a biocompatible sensor with multiple reference electrode areas that are exposed for contact with body fluid.

Abstract translation: 本发明提供一种电化学传感器，其采用暴露于与体液接触的多个电极区域，例如当传感器被皮下插入患者皮肤时。 暴露的电极区域对称地布置，使得当将AC信号施加到传感器时产生对称的电位分布。 根据这些教导的传感器可以有利地与AC信号一起使用以确定传感器的特性，从而提高传感器性能。 这些教导还提供具有暴露于体液接触的多个参考电极区域的生物相容性传感器。

公开(公告)号：US20170000396A9

公开(公告)日：2017-01-05

申请号：US13958404

申请日：2013-08-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Reinhold Mischler ,  Martin Hajnsek ,  Harvey Buck ,  Walter Jernigan

IPC: A61B5/1486

CPC classification number: A61B5/14865 ,  A61B5/14532

Abstract: An in vivo amperometric sensor is provided for measuring the concentration of an analyte in a body fluid. The sensor comprises a counter electrode and a working electrode, and the working electrode comprises a sensing layer which is generally water permeable and arranged on a support member adjacent to a contact pad. The sensing layer comprises an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal. The sensing layer has an upper surface facing the body fluid and a lower surface facing away from the body fluid, and the immobilized enzyme is distributed within the sensing layer in such a way that the enzyme concentration in the middle between the upper and lower surfaces is at least as high as on the upper surface of the sensing layer.

Abstract translation: 提供体内电流传感器用于测量体液中分析物的浓度。 传感器包括对电极和工作电极，并且工作电极包括通常为水可渗透并且布置在与接触垫相邻的支撑构件上的感测层。 感测层包含能够在分析物存在下催化作用以产生电信号的固定化酶。 感测层具有面向体液的上表面和背离体液的下表面，并且固定化酶分布在感测层内，使得上下表面中间的酶浓度为 至少与感测层的上表面一样高。