公开(公告)号：US20170284922A1

公开(公告)日：2017-10-05

申请号：US15509203

申请日：2015-09-01

申请人： SELEXIS S.A.

发明人： Nicolas Mermod ,  Niamh Harraghy ,  Xuan Droz ,  Amar Rida ,  Pierre-Alain Girod ,  Alexandre Regamey ,  Thierry Colombet ,  Etienne Lancon

IPC分类号： G01N15/10 ,  G01N33/543 ,  B01L3/00 ,  C12N5/071 ,  G01N33/569 ,  G01N33/574

CPC分类号： G01N15/1056 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/023 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  C12M47/04 ,  C12N5/0682 ,  C12N2510/02 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/54366 ,  G01N33/56966 ,  G01N33/57492 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081

摘要： The invention discloses a method for selecting cells depending on their level of displaying and preferably secreting a protein of interest from a population of heterogeneously expressing cells, comprising: (a) contacting said cells with magnetic beads coated with an affinity group to the said cells, (b) mixing the said magnetic beads with the cells to capture the cells displaying/secreting the protein of interest, (c) performing at least one washing step to remove the non-captured cells, and (d) recovering the cells to which that magnetic beads have bound.