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    Bioparticle measuring method
    1.
    发明授权
    Bioparticle measuring method 有权

    公开(公告)号：US11592440B2

    公开(公告)日：2023-02-28

    申请号：US16522026

    申请日：2019-07-25

    Applicant: SYSMEX CORPORATION

    Inventor： Takuya Iino ,  Keiko Yoshikawa ,  Kazuya Matsumoto

    IPC: G01N33/536 ,  G01N33/49 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N33/541 ,  G01N15/14 ,  G01N33/68

    Abstract: The present disclosure relates to a bioparticle measuring method including detecting a signal from a first measurement sample and a signal from a second measurement sample, wherein the first measurement sample is prepared by mixing a first sample containing a bioparticle sampled from a specimen with a detector capable of binding to the bioparticle and containing a labeled substance, in the presence of an inhibitor capable of binding to the bioparticle and containing none of the labeled substance. The second measurement sample is prepared by mixing a second sample sampled from the same specimen independently from the first sample with the detector, under a condition that the inhibitor is substantially absent. A measurement result is then calculated from the detected signals from the first and second measurement samples.

    Bioparticle measuring method
    2.
    发明授权
    Bioparticle measuring method 有权

    公开(公告)号：US11525824B2

    公开(公告)日：2022-12-13

    申请号：US16522063

    申请日：2019-07-25

    Applicant: SYSMEX CORPORATION

    Inventor： Kazuya Matsumoto ,  Takuya Iino ,  Keiko Yoshikawa

    IPC: G01N33/541 ,  G01N33/536 ,  G01N33/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N33/49 ,  G01N15/14

    Abstract: The present disclosure relates to a bioparticle measuring method including forming on a solid phase a complex of a sample containing a bioparticle sampled from a specimen, a capturer containing a tag which binds to the solid phase and capable of binding to the bioparticle, and a detector capable of binding to the bioparticle and containing a labeled substance. A part or the whole of the complex may be dissociated from the solid phase to prepare a measurement sample containing a part or the whole of the complex not fixed on the solid phase, and signals from the measurement sample may be detected by a particle analyzer.

    BIOPARTICLE MEASURING METHOD
    5.
    发明申请
    BIOPARTICLE MEASURING METHOD 审中-公开

    公开(公告)号：US20200033365A1

    公开(公告)日：2020-01-30

    申请号：US16522026

    申请日：2019-07-25

    Applicant: SYSMEX CORPORATION

    Inventor： Takuya IINO ,  Keiko Yoshikawa ,  Kazuya Matsumoto

    IPC: G01N33/68 ,  G01N33/53 ,  G01N15/14

    Abstract: Disclosed is a bioparticle measuring method including: detecting signal from a first measurement sample and signal from a second measurement sample, wherein the first measurement sample is prepared by mixing: a first sample containing a bioparticle sampled from a specimen; and a detector capable of binding to the bioparticle and containing a labeled substance, in the presence of an inhibitor capable of binding to the bioparticle and containing none of the labeled substance, and the second measurement sample is prepared by mixing: a second sample sampled from the same specimen independently from the first sample; and the detector, under a condition that the inhibitor is substantially absent; and calculating a measurement result of the bioparticle from a detection result of the signals from the first measurement sample and a detection result of the signals from the second measurement sample.

    Cell information acquisition method and cell information acquisition device
    6.
    发明授权
    Cell information acquisition method and cell information acquisition device 有权

    公开(公告)号：US11022606B2

    公开(公告)日：2021-06-01

    申请号：US15791456

    申请日：2017-10-24

    Applicant: SYSMEX CORPORATION

    Inventor： Keiko Yoshikawa ,  Yuma Oka ,  Shingo Fujiyama

    IPC: G01N33/566 ,  G01N33/53 ,  G01N33/50 ,  G01N15/14 ,  G01N21/64 ,  G01N15/00 ,  G01N15/10

    Abstract: A cell information acquisition method may include: binding a binding substance to a receptor on a cell membrane surface of a cell, the binding substance being bindable to the receptor; permeabilizing a cell membrane of the cell after the binding; labeling a receptor in an intracellular area of the cell with a binding substance labeled with a first labeling substance after the permeabilizing; causing a specimen including the cell to flow through a flow path after the labeling; irradiating the cell included in the specimen flowing through the flow path with light; and acquiring a signal based on light generated from the first labeling substance in the cell irradiated with light.

    Bioparticle measuring method
    8.
    发明授权
    Bioparticle measuring method 有权

    公开(公告)号：US11346838B2

    公开(公告)日：2022-05-31

    申请号：US16522063

    申请日：2019-07-25

    Applicant: SYSMEX CORPORATION

    Inventor： Kazuya Matsumoto ,  Takuya Iino ,  Keiko Yoshikawa

    IPC: G01N33/541 ,  G01N33/536 ,  G01N33/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N33/49 ,  G01N15/14

    Abstract: The present disclosure relates to a bioparticle measuring method including forming on a solid phase a complex of a sample containing a bioparticle sampled from a specimen, a capturer containing a tag which binds to the solid phase and capable of binding to the bioparticle, and a detector capable of binding to the bioparticle and containing a labeled substance. A part or the whole of the complex may be dissociated from the solid phase to prepare a measurement sample containing a part or the whole of the complex not fixed on the solid phase, and signals from the measurement sample may be detected by a particle analyzer.

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