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    APPARATUS AND PROCESSES FOR ISOLATING BIOLOGICAL MATERIAL
    1.
    发明申请
    APPARATUS AND PROCESSES FOR ISOLATING BIOLOGICAL MATERIAL 有权

    公开(公告)号：US20220090000A1

    公开(公告)日：2022-03-24

    申请号：US17484381

    申请日：2021-09-24

    Applicant: UNIVERSITY OF WYOMING

    Inventor： John OAKEY ,  John KISIDAY ,  Benjamin NOREN

    IPC: C12M1/00 ,  C12M1/32 ,  C12M3/06 ,  B01L3/00

    Abstract: Embodiments of the present disclosure generally relate to apparatus for isolating biological material, processes for fabricating such apparatus, and processes for using such apparatus. In an embodiment, an apparatus for isolating a biological material is provided. The apparatus includes a fluidic channel disposed over a portion of a substrate. The apparatus further includes a hydrogel structure disposed in the fluidic channel, the hydrogel structure comprising a plurality of wells, wherein each well of the plurality of wells has a diameter from about 1 μm to about 500 μm, the hydrogel structure comprising, in polymerized form, one or more photoreactive monomers.

    HYDROGEL-ENCAPSULATED CELLS AND HYDROGEL-DISPERSED CELLS
    3.
    发明申请
    HYDROGEL-ENCAPSULATED CELLS AND HYDROGEL-DISPERSED CELLS 有权

    公开(公告)号：US20220064624A1

    公开(公告)日：2022-03-03

    申请号：US17463362

    申请日：2021-08-31

    Applicant: UNIVERSITY OF WYOMING

    Inventor： Benjamin NOREN ,  John OAKEY ,  Zhongliang JIANG ,  Kun JIANG

    IPC: C12N11/089 ,  C12N11/087

    Abstract: Embodiments of the present disclosure generally relate to compositions that include hydrogel-encapsulated/dispersed cells, compositions including hydrogel-encapsulated/dispersed cells, and to processes for forming such hydrogel-encapsulated/dispersed cells and compositions thereof. The compositions can be used for, e.g., therapeutic applications. In some examples, the hydrogel-encapsulated/dispersed cells are formed using photoreactive groups chemically attached to polyethylene glycol to form a material which, upon exposure to a desired wavelength or wavelength range of light, reacts to form a cross-linked hydrogel network.

    FIBRIN PARTICLES AND METHODS OF FORMING FIBRIN PARTICLES
    4.
    发明申请
    FIBRIN PARTICLES AND METHODS OF FORMING FIBRIN PARTICLES 有权

    公开(公告)号：US20210171587A1

    公开(公告)日：2021-06-10

    申请号：US17112661

    申请日：2020-12-04

    Applicant: UNIVERSITY OF WYOMING

    Inventor： John OAKEY ,  Alan STENQUIST

    IPC: C07K14/435 ,  C07K14/75

    Abstract: The present disclosure generally relates to compositions comprising fibrin and to methods of forming such compositions. In an embodiment, a method of forming fibrin particles is provided. The method includes introducing a buffer, a fibrinogen solution, and a thrombin solution to a first end of a microfluidic device to form a mixture, the buffer comprising one or more amino acids. The method further includes contacting the mixture with a fluorocarbon oil and a surfactant to form fibrinogen-containing particles, and applying positive pressure to the microfluidic device to cause the fibrinogen-containing particles to flow towards a second end of the microfluidic device. The method further includes collecting the fibrinogen-containing particles at the second end of the microfluidic device; and polymerizing the fibrinogen-containing particles to form fibrin particles.

    FIBRIN PARTICLES AND METHODS OF FORMING FIBRIN PARTICLES
    5.
    发明公开
    FIBRIN PARTICLES AND METHODS OF FORMING FIBRIN PARTICLES 审中-公开

    公开(公告)号：US20240317823A1

    公开(公告)日：2024-09-26

    申请号：US18737141

    申请日：2024-06-07

    Applicant: UNIVERSITY OF WYOMING

    Inventor： John OAKEY ,  Alan STENQUIST

    IPC: C07K14/435 ,  C07K14/75

    CPC classification number: C07K14/435 ,  C07K14/75

    Abstract: The present disclosure generally relates to compositions comprising fibrin and to methods of forming such compositions. In an embodiment, a method of forming fibrin particles is provided. The method includes introducing a buffer, a fibrinogen solution, and a thrombin solution to a first end of a microfluidic device to form a mixture, the buffer comprising one or more amino acids. The method further includes contacting the mixture with a fluorocarbon oil and a surfactant to form fibrinogen-containing particles, and applying positive pressure to the microfluidic device to cause the fibrinogen-containing particles to flow towards a second end of the microfluidic device. The method further includes collecting the fibrinogen-containing particles at the second end of the microfluidic device; and polymerizing the fibrinogen-containing particles to form fibrin particles.

    EXPLOITING OXYGEN INHIBITED PHOTOPOLYMERIZATION WITHIN EMULSION DROPLETS FOR THE FABRICATION OF MICROPARTICLES WITH CUSTOMIZABLE PROPERTIES
    7.
    发明申请
    EXPLOITING OXYGEN INHIBITED PHOTOPOLYMERIZATION WITHIN EMULSION DROPLETS FOR THE FABRICATION OF MICROPARTICLES WITH CUSTOMIZABLE PROPERTIES 审中-公开

    公开(公告)号：US20200254417A1

    公开(公告)日：2020-08-13

    申请号：US16647869

    申请日：2018-10-17

    Applicant: University of Wyoming

    Inventor： John OAKEY ,  Katie Dongmei LI-OAKEY ,  Daniel DEBROY ,  Carl FRICK ,  Rajib SHAHA

    IPC: B01J13/14 ,  B01J13/00 ,  A61K9/16

    Abstract: Described are methods and devices for the generation of hydrogel particles with micrometer and submicrometer dimensions using oxygen-inhibited partial polymerization, and the particles generated therefrom. The described methods generate particles with dimensions independent of the starting polymerizable solution dimension, for example, a microdroplet. Further, microfluidic flow parameters (e.g. viscosity, flow rate) and photopolymerization process parameters (e.g. optical exposure intensity and duration) are controlled to generate particles with tunable crosslinking density-determined properties including elasticity, diffusivity, and biomolecular display for diverse applications such as drug delivery, tissue engineering cell scaffolds, and single- and multiple-cell therapeutics. Similarly, gradients of crosslinking density-determined properties can be created within single particles through the selection of optical exposure intensity and duration. In addition to conventional spherical shapes, a suite of non-spherical shapes may be generated by manipulating the dimensions of the microfluidic channels and other related physical and process parameters.

    CONVECTIVE FLOW-DRIVEN MICROFABRICATED HYDROGELS FOR RAPID BIOSENSING
    10.
    发明申请
    CONVECTIVE FLOW-DRIVEN MICROFABRICATED HYDROGELS FOR RAPID BIOSENSING 有权

    公开(公告)号：US20210025881A1

    公开(公告)日：2021-01-28

    申请号：US16936208

    申请日：2020-07-22

    Applicant: UNIVERSITY OF WYOMING

    Inventor： John OAKEY ,  Mark H. HARPSTER ,  Cheng CHENG

    IPC: G01N33/543 ,  G01N33/58 ,  G01N21/65

    Abstract: Embodiments of the present disclosure generally relate to apparatus and methods for analyte detection. More specifically, embodiments of the present disclosure relate to a microscale biosensing platform based upon the rehydration-mediated swelling of functionalized hydrogel structures and rapid capture of target analyte(s) by induced convective flow. In an embodiment is provided an apparatus for analyte detection that includes a fluidic channel coupled to a substrate and a hydrogel structure coupled to the fluidic channel. The hydrogel structure includes one or more surface-functionalized hydrogel features, wherein at least one of the one or more surface-functionalized hydrogel features comprises a chemically-bound probe, the chemically-bound probe to bind an analyte, and wherein the hydrogel structure is at least partially dehydrated. Apparatus described herein can also include a detector. Methods of detecting analytes are also described herein.

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