公开(公告)号：US20240368583A1

公开(公告)日：2024-11-07

申请号：US18765281

申请日：2024-07-07

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David MARGULIES ,  Leila MOTIEI ,  Suraj Uttamrao Toraskar ,  Abhirup Majumdar

IPC: C12N15/10 ,  C12N1/20 ,  C12N15/11 ,  G01N33/574 ,  G01N33/58

Abstract: The disclosure presented herein provides DNA constructs, recombinant cells comprising thereof, system comprising thereof, bacterial probes, and/or a recombinant cell decorated with various labels and/or synthetic agents, wherein said labels and/or synthetic agents can be reversibly modified or removed from the cells. Also disclosed herein are methods for decorating and/or modifying cells, preferably bacteria cells, and methods for using thereof.

公开(公告)号：US20210156867A1

公开(公告)日：2021-05-27

申请号：US17140075

申请日：2021-01-03

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David MARGULIES ,  Leila MOTIEI ,  Pragati PRASAD ,  Tom GRANOT ,  Ohad SUSS

IPC: G01N33/58 ,  G01N33/68 ,  G01N21/64 ,  G01N33/50

Abstract: This invention is directed to a turn-on fluorescent probe, wherein said probe comprises a quinoline based cyanine dye derivative (QBC) and a specific protein binder covalently attached thereto directly or via a linker. This invention is further directed to His-tag binding compounds and uses thereof in the preparation of genetically targeted detectable molecules and sensors which can specifically bind tag-labeled proteins. This invention further describes methods for using these turn-on probes and compounds for detecting and labelling a wide range of proteins in their native environment.

公开(公告)号：US20200256857A1

公开(公告)日：2020-08-13

申请号：US16783524

申请日：2020-02-06

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David MARGULIES ,  Leila MOTIEI ,  Naama LAHAV

IPC: G01N33/533 ,  C07D249/04 ,  G01N33/58 ,  G01N33/68

Abstract: This invention is directed to His-tag binding compounds and uses thereof in the preparation of genetically targeted detectable molecules and sensors which can specifically bind tag-labeled proteins. This invention further provides a system comprising recombinant cells decorated with various labels and/or synthetic agents, wherein said labels and/or synthetic agents can be reversibly modified or removed from the cells. Also disclosed herein are methods for decorating and/or modifying the cells and methods for using thereof.

公开(公告)号：US20170045522A1

公开(公告)日：2017-02-16

申请号：US15307011

申请日：2015-04-28

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David MARGULIES ,  Leila MOTIEI ,  Naama MANKOVSKI ,  Yael NISSINKORN

IPC: G01N33/58 ,  G01N33/68

Abstract: This invention is directed to monomolecular sensors, comprising a selective binder, a non-selective binder and a fluorophore, which can track changes that occur on the surface of labeled proteins. This invention is further directed to the use of such sensors for identifying binding partners of specific proteins. This invention is further directed to His-tag binding compounds and uses thereof in the preparation of genetically targeted detectable molecules and sensors which can specifically bind tag-labeled proteins.

Abstract translation: 本发明涉及单分子传感器，其包括选择性粘合剂，非选择性粘合剂和荧光团，其可以跟踪标记蛋白质表面上发生的变化。 本发明还涉及这种传感器用于鉴定特定蛋白质的结合伴侣的用途。 本发明进一步涉及His-标签结合化合物及其在制备可特异性结合标签标记的蛋白质的遗传可靶向可检测分子和传感器的用途。

公开(公告)号：US20150276753A1

公开(公告)日：2015-10-01

申请号：US14738894

申请日：2015-06-14

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David MARGULIES ,  Leila Motiei ,  Linor Unger

IPC: G01N33/58 ,  C07F9/6541 ,  C07D417/06 ,  C07D417/14 ,  G01N33/573

CPC classification number: G01N33/573 ,  C07D417/06 ,  C07D417/14 ,  C07F9/6541 ,  C07K7/06 ,  C09K11/06 ,  C09K2211/1029 ,  C09K2211/1037 ,  G01N33/574 ,  G01N33/582 ,  G01N2333/91177 ,  G01N2333/918 ,  G01N2333/96494

Abstract: The present invention is directed to fluorescent molecular sensor based on Thiazole Orange for protein detection. Interaction of the protein target with the molecular sensors of this invention results in a significant increase in the fluorescence emission. The generation of light output signal enables one to detect protein biomarkers associated with different diseases or detecting the protein of interest also in living cells.

Abstract translation: 本发明涉及基于噻唑橙用于蛋白质检测的荧光分子传感器。 蛋白质靶标与本发明的分子传感器的相互作用导致荧光发射的显着增加。 光输出信号的产生使得人们能够检测与不同疾病相关的蛋白质生物标志物或检测活细胞中感兴趣的蛋白质。

公开(公告)号：US20230366885A1

公开(公告)日：2023-11-16

申请号：US18350066

申请日：2023-07-11

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David MARGULIES ,  Leila Motiei ,  Naama Lahav ,  Pragati Prasad

IPC: G01N33/58 ,  G01N33/574

CPC classification number: G01N33/582 ,  G01N33/574

Abstract: The disclosure presented herein provides DNA constructs, recombinant cells comprising thereof, system comprising thereof, bacterial probes, and/or a recombinant cell decorated with various labels and/or synthetic agents, wherein said labels and/or synthetic agents can be reversibly modified or removed from the cells. Also disclosed herein are methods for decorating and/or modifying cells, preferably bacteria cells, and methods for using thereof.

公开(公告)号：US20220236257A1

公开(公告)日：2022-07-28

申请号：US17614563

申请日：2019-06-05

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David MARGULIES ,  Leila MOTIEI ,  Naama LAHAV

IPC: G01N33/532 ,  C07K14/705 ,  G01N33/50 ,  C12N5/00 ,  C12N1/00 ,  C12Q1/02

Abstract: The disclosure presented herein provides artificial receptors and a system comprising recombinant cells decorated with various labels and/or synthetic agents, wherein said labels and/or synthetic agents can be reversibly modified or removed from the cells. Also disclosed herein are methods for decorating and/or modifying the cells and methods for using thereof.

公开(公告)号：US20170307625A1

公开(公告)日：2017-10-26

申请号：US15640545

申请日：2017-07-02

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David MARGULIES ,  Leila MOTIEI ,  Linor UNGER

IPC: G01N33/58 ,  C07D417/14 ,  C09K11/06 ,  C07D417/06 ,  G01N33/573 ,  C07K7/06 ,  G01N33/574

CPC classification number: G01N33/582 ,  C07D417/06 ,  C07D417/14 ,  C07F9/65583 ,  C07K7/06 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/23 ,  C09K11/06 ,  C09K2211/1029 ,  C09K2211/1037 ,  C12N9/1088 ,  G01N33/573 ,  G01N33/574 ,  G01N2333/91177 ,  G01N2333/918 ,  G01N2333/96494

Abstract: The present invention is directed to fluorescent molecular sensor based on Thiazole Orange for protein detection. interaction of the protein target with the molecular sensors of this invention results in a significant increase in the fluorescence emission. The generation of light output signal enables one to detect protein biomarkers associated with different diseases or detecting the protein of interest also in living cells.

公开(公告)号：US20150362490A1

公开(公告)日：2015-12-17

申请号：US14754785

申请日：2015-06-30

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David MARGULIES ,  Leila Motiei ,  Zohar Pode

IPC: G01N33/573 ,  C07K7/06

CPC classification number: G01N33/53 ,  C07D417/06 ,  C07D417/14 ,  C07F9/6541 ,  C07K7/06 ,  C09K11/06 ,  C09K2211/1029 ,  C09K2211/1037 ,  G01N33/573 ,  G01N33/574 ,  G01N33/582 ,  G01N2333/91177 ,  G01N2333/918 ,  G01N2333/96494

Abstract: The present invention is directed to fluorescent protein biosensors based on oligonucleotide cross reactive sensor arrays including a selective and a non-selective protein surface binding domain for protein detection. Interaction of different protein isoforms with the sensors of this invention yields a unique optical signature for each protein. The unique optical signature allows differentiating between closely related protein isoforms and diagnosing diseases and disorders associated with the proteins also in biofluids.

Abstract translation: 本发明涉及基于寡核苷酸交叉反应性传感器阵列的荧光蛋白生物传感器，其包括用于蛋白质检测的选择性和非选择性蛋白质表面结合结构域。 不同蛋白质同种型与本发明传感器的相互作用产生了每种蛋白质独特的光学特征。 独特的光学特征允许区分密切相关的蛋白质同种型，并在生物流体中诊断与蛋白质相关的疾病和病症。