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    Method for production of polyester copolymer using recombinant microorganism
    7.
    发明授权
    Method for production of polyester copolymer using recombinant microorganism 有权
    使用重组微生物生产聚酯共聚物的方法

    公开(公告)号：US08563281B2

    公开(公告)日：2013-10-22

    申请号：US12989333

    申请日：2009-04-23

    申请人： Seiichi Taguchi ,  Kenji Tajima ,  Yasuharu Satoh ,  Ken'ichiro Matsumoto ,  Miwa Yamada ,  Shusei Obata ,  Hiromi Kambe ,  Katsunori Kohda ,  Katsuhiro Ohno ,  Takashi Shimamura

    发明人： Seiichi Taguchi ,  Kenji Tajima ,  Yasuharu Satoh ,  Ken'ichiro Matsumoto ,  Miwa Yamada ,  Shusei Obata ,  Hiromi Kambe ,  Katsunori Kohda ,  Katsuhiro Ohno ,  Takashi Shimamura

    IPC分类号： C12P7/62 ,  C12N1/20 ,  C12N1/00

    CPC分类号： C12P7/62

    摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate via microbial fermentation with the use of a sugar as a starting material. The method may comprise culturing a recombinant microorganism expressing: a protein capable of catalyzing the transfer of CoA to propionic acid and/or lactate; a protein capable of catalyzing the formation of acetoacetyl-CoA from two acetyl-CoA molecules; a protein capable of catalyzing acetoacetyl-CoA reduction; and a protein capable of catalyzing polyhydroxyalkanoate synthesis. According to the production method, a polyester copolymer consisting of 3-hydroxybutyrate and lactate can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.

    摘要翻译： 本发明的目的是提供一种通过使用糖作为原料通过微生物发酵有效地制备由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的方法。 该方法可以包括培养表达：能够催化CoA转移到丙酸和/或乳酸盐的蛋白质的重组微生物; 能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的蛋白质; 能够催化乙酰乙酰辅酶A还原的蛋白质; 和能够催化聚羟基链烷酸酯合成的蛋白质。 根据该制造方法，可以使用廉价的碳源作为起始材料有效地制造由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

    Microorganism capable of producing polyhydroxyalkanoate, polyhydroxyalkanoate synthase, and gene encoding the same
    10.
    发明申请
    Microorganism capable of producing polyhydroxyalkanoate, polyhydroxyalkanoate synthase, and gene encoding the same 失效
    能够产生聚羟基链烷酸酯，聚羟基链烷酸酯合成酶的微生物和编码它们的基因

    公开(公告)号：US20100035313A1

    公开(公告)日：2010-02-11

    申请号：US12459941

    申请日：2009-07-09

    申请人： Yasuharu Satou ,  Kenji Tajima ,  Masanobu Munekata ,  Tokuo Matsushima

    发明人： Yasuharu Satou ,  Kenji Tajima ,  Masanobu Munekata ,  Tokuo Matsushima

    IPC分类号： C12P7/62 ,  C12N1/00 ,  C12N1/20 ,  C07H21/00 ,  C12N15/63 ,  C12N9/00

    CPC分类号： C12P7/625 ,  C12N9/1029

    摘要： Problem to be Solved: To provide a new microorganism capable of producing a polyhydroxyalkanoate (PHA), a PHA synthase gene, an expression cassette including the gene, a vector including the expression cassette, a transformant transformed by the vector, a polypeptide having PHA synthase activity, a method for producing a PHA synthase and a method for producing a PHA.Solution: The new microorganism is capable of producing a polyhydroxyalkanoate comprising a 16S rRNA gene whose polynucleotide sequence shows 99% or more homology to a polynucleotide sequence represented by SEQ ID No: 1, having an optimum temperature of an activity temperature range for the growth and PHA production of the microorganism of at least 45° C. and being capable of growing at a pH range from 6 to 10.

    摘要翻译： 要解决的问题：提供能够产生聚羟基链烷酸酯（PHA），PHA合酶基因，包含该基因的表达盒，包含表达盒的载体，由载体转化的转化体的新微生物，具有PHA合酶的多肽 活性，生产PHA合成酶的方法和制备PHA的方法。 解决方案：新的微生物能够生产包含16S rRNA基因的聚羟基链烷酸酯，其多核苷酸序列与SEQ ID No：1所代表的多核苷酸序列具有99％或更高的同源性，具有生长活性温度范围的最适温度， PHA生产至少45℃的微生物，并能够在6至10的pH范围内生长。