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    Adenovector complementing cells
    91.
    发明申请
    Adenovector complementing cells 有权
    Adenovector补体细胞

    公开(公告)号：US20030054553A1

    公开(公告)日：2003-03-20

    申请号：US09910828

    申请日：2001-07-23

    Applicant: GenVec, Inc.

    Inventor： Douglas E. Brough ,  Jason G.D. Gall ,  Imre Kovesdi

    IPC: C12N015/861 ,  C12N007/00 ,  C12N005/08

    CPC classification number: C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352

    Abstract: The invention provides cells, particularly NCI-H460 cells and Calu-1 cells, for the propagation of replication-deficient adenoviral vectors. The cells are lung carcinomas with either a wild-type p53 gene or a heterozygous K-ras mutation. The cells comprise at least one adenoviral nucleic acid sequence, which upon expression produces a gene product that complements for at least one essential gene function of one or more regions of an adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the one or more regions when present in the cell.

    Abstract translation: 本发明提供用于增殖复制缺陷型腺病毒载体的细胞，特别是NCI-H460细胞和Calu-1细胞。 细胞是具有野生型p53基因或杂合K-ras突变的肺癌。 细胞包含至少一种腺病毒核酸序列，其在表达时产生补充腺病毒基因组的一个或多个区域的至少一个必需基因功能的基因产物，以便繁殖包含腺病毒基因组的复制缺陷型腺病毒载体 当存在于细胞中时，一个或多个区域的至少一个必需基因功能缺陷。

    Targeting adenovirus with use of constrained peptide motifs
    93.
    发明申请
    Targeting adenovirus with use of constrained peptide motifs 有权
    使用受限肽基序靶向腺病毒

    公开(公告)号：US20020151027A1

    公开(公告)日：2002-10-17

    申请号：US09969192

    申请日：2001-10-01

    Applicant: GenVec, Inc.

    Inventor： Thomas J. Wickham ,  Petrus W. Roelvink ,  Imre Kovesdi

    IPC: C12N015/861 ,  C12N009/50 ,  C12N007/01

    CPC classification number: C12N7/00 ,  A61K38/00 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2810/40 ,  C12N2810/405 ,  C12N2810/60

    Abstract: The present invention provides a chimeric adenovirus fiber protein, which differs from the wild-type coat protein by the introduction of a nonnative amino acid sequence in a conformationally-restrained manner. Such a chimeric adenovirus fiber protein according to the invention is able to direct entry into cells of a vector comprising the chimeric fiber protein that is more efficient than entry into cells of a vector that is identical except for comprising a wild-type adenovirus fiber protein rather than the chimeric adenovirus fiber protein. The nonnative amino acid sequences encodes a peptide motif that comprises an epitope for an antibody, or a ligand for a cell surface receptor, that can be employed in cell targeting. The present invention also pertains to vectors comprising such a chimeric adenovirus fiber protein, and to methods of using such vectors.

    Abstract translation: 本发明提供了一种嵌合腺病毒纤维蛋白，其通过以构象约束的方式引入非氨基酸序列而与野生型外壳蛋白不同。 根据本发明的这种嵌合腺病毒纤维蛋白能够直接进入包含嵌合纤维蛋白质的载体的细胞，该载体比除了包含野生型腺病毒纤维蛋白的载体的细胞进入更有效，而不是包含野生型腺病毒纤维蛋白 比嵌合腺病毒纤维蛋白。 非氨基酸序列编码包含可用于细胞靶向的抗体表位或细胞表面受体配体的肽基序。 本发明还涉及包含这种嵌合腺病毒纤维蛋白的载体，以及使用这些载体的方法。

    Methods of administering adenoviral vectors
    95.
    发明申请
    Methods of administering adenoviral vectors 失效
    腺病毒载体的施用方法

    公开(公告)号：US20020013286A1

    公开(公告)日：2002-01-31

    申请号：US09835683

    申请日：2001-04-16

    Applicant: GenVec, Inc.

    Inventor： Joseph T. Bruder ,  Imre Kovesdi

    IPC: A61K048/00 ,  A61K039/395

    CPC classification number: A61K48/00 ,  A61K2039/505 ,  C07K16/081

    Abstract: The present invention provides methods for administering an adenoviral gene transfer vector comprising an exogenous gene to an animal. One method involves utilizing systemic neutralizing antibodies to neutralize the adenoviral gene transfer vector outside a targeted muscle. Another method involves the repeat administration of an adenoviral gene transfer vector to a skeletal muscle.

    Abstract translation: 本发明提供了向动物施用包含外源基因的腺病毒基因转移载体的方法。 一种方法涉及利用全身中和抗体来中和靶向肌肉外的腺病毒基因转移载体。 另一种方法涉及向骨骼肌重复施用腺病毒基因转移载体。

    Methods of preparing and using a viral vector library
    96.
    发明申请
    Methods of preparing and using a viral vector library 有权
    制备和使用病毒载体文库的方法

    公开(公告)号：US20010026794A1

    公开(公告)日：2001-10-04

    申请号：US09780526

    申请日：2001-02-09

    Applicant: GenVec, Inc.

    Inventor： Imre Kovesdi ,  Duncan L. McVey ,  Thomas J. Wickham ,  Joseph T. Bruder ,  Douglas E. Brough

    IPC: A61K048/00 ,  C12Q001/70 ,  C12N007/01

    CPC classification number: C12N15/1093 ,  A61K48/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2810/40

    Abstract: The present invention provides a library of viral vectors, wherein each member comprises a first heterologous DNA encoding a first gene product and a second heterologous DNA encoding a second gene product. The first heterologous DNA is common to each member of the library, while the second heterologous DNA varies between members of the library. The present invention additionally provides a method of constructing a library of viral vectors. The method comprises carrying out homologous recombination between a first DNA molecule and a second DNA molecule to form a pool of intermediate viral vector genomes. One or more linear third DNA molecules are ligated into the pool of intermediate viral genomes to produce a library of viral vector genomes. Alternatively, homologous recombination between linear DNA molecules and recipient DNA molecules produces a library of viral vector genomes. The library of viral vector genomes is converted into a library of viral vectors.

    Abstract translation: 本发明提供病毒载体文库，其中每个成员包含编码第一基因产物的第一异源DNA和编码第二基因产物的第二异源DNA。 第一个异源DNA对于文库的每个成员是共同的，而第二异源DNA在文库的成员之间是不同的。 本发明另外提供构建病毒载体文库的方法。 该方法包括在第一DNA分子和第二DNA分子之间进行同源重组以形成中间病毒载体基因组池。 将一个或多个线性第三DNA分子连接到中间病毒基因组池中以产生病毒载体基因组文库。 或者，线性DNA分子和受体DNA分子之间的同源重组产生病毒载体基因组文库。 将病毒载体基因组文库转化为病毒载体文库。

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