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170 条记录 (耗时 0.069 秒)

    Method for serial analysis of gene expression
    96.
    发明授权
    Method for serial analysis of gene expression 失效
    基因表达序列分析方法

    公开(公告)号：US06746845B2

    公开(公告)日：2004-06-08

    申请号：US10096596

    申请日：2002-03-14

    申请人： Kenneth W. Kinzler ,  Bert Vogelstein ,  Victor E. Velculescu ,  Lin Zhang

    发明人： Kenneth W. Kinzler ,  Bert Vogelstein ,  Victor E. Velculescu ,  Lin Zhang

    IPC分类号： C12Q168

    CPC分类号： C12N15/1096 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q2539/103

    摘要： Serial analysis of gene expression, SAGE, a method for the rapid quantitative and qualitative analysis of transcripts is provided. Short defined sequence tags corresponding to expressed genes are isolated and analyzed. Sequencing of over 1,000 defined tags in a short period of time (e.g., hours) reveals a gene expression pattern characteristic of the function of a cell or tissue. Moreover, SAGE is useful as a gene discovery tool for the identification and isolation of novel sequence tags corresponding to novel transcripts and genes.

    摘要翻译： 提供基因表达的串行分析，SAGE，一种用于快速定量和定性分析转录本的方法。 分离并分析与表达基因相对应的短定义序列标签。 在短时间（例如，小时）内测定超过1,000个定义的标签显示了细胞或组织功能特征的基因表达模式。 此外，SAGE可用作用于鉴定和分离对应于新转录物和基因的新序列标签的基因发现工具。

    P53-induced apoptosis
    97.
    发明授权
    P53-induced apoptosis 有权
    P53诱导凋亡

    公开(公告)号：US06432640B1

    公开(公告)日：2002-08-13

    申请号：US09154750

    申请日：1998-09-17

    申请人： Kornelia Polyak ,  Bert Vogelstein ,  Kenneth W. Kinzler

    发明人： Kornelia Polyak ,  Bert Vogelstein ,  Kenneth W. Kinzler

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6809 ,  C12Q1/6886 ,  C12Q1/6897 ,  C12Q2539/103 ,  C12Q2600/136 ,  C12Q2600/142

    摘要： The most well-documented biochemical property of p53 is its ability to transcriptionally activate genes. Many of the genes which are activated by p53 expression prior to the onset of apoptosis are predicted to encode proteins which could generate or respond to oxidative stress, including one that is implicated in apoptosis within plant meristems. p53 may result in apoptosis through a three-step process: (I) the transcriptional induction of specific redox-related genes; (ii) the formation of reactive oxygen species (ROS); and (iii) the oxidative degradation of mitochondrial components, rapidly leading to cell death. Transcription of other genes is decreased by p53. Examination of the level of transcription of p53-induced or repressed genes can be used to determine p53 status, to diagnose cancer, and to evaluate cytotoxicity or carcinogenicity of a test agent.

    摘要翻译： 最有说明的p53生物化学性质是其转录激活基因的能力。 预计在细胞凋亡发生之前由p53表达激活的许多基因可以编码可产生或响应氧化应激的蛋白质，包括与植物分生组织内凋亡相关的蛋白质。 p53可能通过三步过程导致凋亡：（I）特异性氧化还原相关基因的转录诱导; （ii）形成活性氧（ROS）; 和（iii）线粒体成分的氧化降解，迅速导致细胞死亡。 其他基因的转录通过p53降低。 检测p53诱导或抑制基因的转录水平可用于确定p53状态，诊断癌症，并评估测试药物的细胞毒性或致癌性。

    Gene expression profiles in normal and cancer cells
    98.
    发明授权
    Gene expression profiles in normal and cancer cells 失效
    正常和癌细胞中的基因表达谱

    公开(公告)号：US06333152B1

    公开(公告)日：2001-12-25

    申请号：US09081646

    申请日：1998-05-20

    申请人： Bert Vogelstein ,  Kenneth W. Kinzler ,  Lin Zhang ,  Wei Zhou

    发明人： Bert Vogelstein ,  Kenneth W. Kinzler ,  Lin Zhang ,  Wei Zhou

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/574

    摘要： As a step towards understanding the complex differences between normal and cancer cells, gene expression patterns were examined in gastrointestinal tumors. More than 300,000 transcripts derived from at least 45,000 different genes were analyzed. Although extensive similarity was noted between the expression profiles, more than 500 transcripts that were expressed at significantly different levels in normal and neoplastic cells were identified. These data provide insights into the extent of expression differences underlying malignancy and reveal genes that are useful as diagnostic or prognostic markers.

    摘要翻译： 作为理解正常和癌细胞之间复杂差异的一步，在胃肠道肿瘤中检查基因表达模式。 分析来自至少45,000个不同基因的超过30万个转录物。 虽然在表达谱之间注意到广泛的相似性，但是鉴定了在正常和肿瘤细胞中以显着不同水平表达的500多个转录物。 这些数据提供了对恶性肿瘤的表达差异程度的了解，并揭示了可用作诊断或预后标志物的基因。

    Mad-related genes in the human
    99.
    发明授权
    Mad-related genes in the human 失效
    人类中与Mad相关的基因

    公开(公告)号：US06255464B1

    公开(公告)日：2001-07-03

    申请号：US08840767

    申请日：1997-04-16

    申请人： Bert Vogelstein ,  Kenneth W. Kinzler ,  Gregory R. Riggins ,  Sam Thiagalingam

    发明人： Bert Vogelstein ,  Kenneth W. Kinzler ,  Gregory R. Riggins ,  Sam Thiagalingam

    IPC分类号： C07H2102

    CPC分类号： C07K14/4703 ,  A61K38/00

    摘要： Five human genes related to the Mad gene of Drosophila were identified. One of these genes (Smad2) was found to reside at chromosome 18q21, adjacent to a previously described member of this family called DPC4 (Smad4). Smad2 was found to be somatically mutated in two of eighteen human colorectal cancers. Smad2 and Smad4 are important in the suppression of neoplasia by mediating the growth inhibitory effects of TGF-&bgr;-like ligands.

    摘要翻译： 鉴定了与果蝇Mad基因相关的五个人类基因。 发现这些基因之一（Smad2）位于染色体18q21处，与该家族之前称为DPC4（Smad4）的成员相邻。 发现Smad2在十八个人结肠直肠癌中的两个中被体细胞突变。 Smad2和Smad4通过介导TGF-β样配体的生长抑制作用来抑制肿瘤是重要的。

    Simplified system for generating recombinant adenoviruses
    100.
    发明授权
    Simplified system for generating recombinant adenoviruses 失效
    用于产生重组腺病毒的简化系统

    公开(公告)号：US5922576A

    公开(公告)日：1999-07-13

    申请号：US31917

    申请日：1998-02-27

    申请人： Tong-Chuan He ,  Bert Vogelstein ,  Kenneth W. Kinzler

    发明人： Tong-Chuan He ,  Bert Vogelstein ,  Kenneth W. Kinzler

    IPC分类号： C07K14/075 ,  C12N15/861 ,  C12N15/64 ,  C07H21/04 ,  C12N1/21 ,  C12N15/70

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2830/38

    摘要： Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. This invention describes a strategy which simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production can be conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system expedites the process of generating and testing recombinant adenoviruses.

    摘要翻译： 重组腺病毒为基因表达研究和治疗应用提供了多功能的系统。 本发明描述了一种简化这种病毒生成和生产的策略。 通过最少的酶操作产生重组腺病毒质粒，在细菌中而不是在真核细胞中使用同源重组。 在将这些质粒转染到哺乳动物包装细胞系之后，可以方便地利用由掺入病毒骨架的基因编码的绿色荧光蛋白来辅助病毒生产。 可以从该程序获得均一的病毒，无需进行噬斑纯化。 该系统加速了重组腺病毒的产生和检测过程。