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126 条记录 (耗时 0.056 秒)

    2-O sulfatase nucleic acid compositions
    93.
    发明授权
    2-O sulfatase nucleic acid compositions 有权
    2-O硫酸酯酶核酸组合物

    公开(公告)号：US07396824B2

    公开(公告)日：2008-07-08

    申请号：US11433224

    申请日：2006-05-11

    申请人： Ram Sasisekharan ,  James Myette ,  Zachary Shriver ,  Ganesh Venkataraman

    发明人： Ram Sasisekharan ,  James Myette ,  Zachary Shriver ,  Ganesh Venkataraman

    IPC分类号： A61K31/70 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N9/16 ,  A61K38/00 ,  C12P19/04 ,  G01N33/5041 ,  G01N2400/40

    摘要： The invention relates to 2-O sulfatase and uses thereof. In particular, the invention relates to recombinantly produced 2-O sulfatase, functional variants and nucleic acid molecules that encode these molecules. The invention also provides methods of using 2-O sulfatase for a variety of purposes, including degrading and analyzing glycosaminoglycans (GAGs) present in a sample. For instance, 2-O sulfatase may be used for determining the purity, identity, composition and sequence of glycosaminoglycans present in a sample. The invention also relates to methods of inhibiting angiogenesis and cellular proliferation as well as methods for treating cancer, neurodegenerative disease, atherosclerosis and microbial infection using 2-O sulfatase and/or GAG fragments produced by degradation with 2-O sulfatase.

    摘要翻译： 本发明涉及2-O硫酸酯酶及其用途。 特别地，本发明涉及编码这些分子的重组产生的2-O硫酸酯酶，功能变体和核酸分子。 本发明还提供了用于各种目的的2-O硫酸酯酶的方法，包括降解和分析样品中存在的糖胺聚糖（GAG）。 例如，2-O硫酸酯酶可用于测定样品中存在的糖胺聚糖的纯度，同一性，组成和顺序。 本发明还涉及抑制血管生成和细胞增殖的方法以及使用2-O硫酸酯酶降解产生的2- O硫酸酯酶和/或GAG片段来治疗癌症，神经变性疾病，动脉粥样硬化和微生物感染的方法。

    Monitoring heparin by microelectronic devices
    95.
    发明申请
    Monitoring heparin by microelectronic devices 审中-公开
    通过微电子器件监测肝素

    公开(公告)号：US20070212786A1

    公开(公告)日：2007-09-13

    申请号：US11540695

    申请日：2006-09-29

    申请人： Scott Manalis ,  Tzu Loh ,  Michel Godin ,  Nebojsa Milovic ,  Jonathan Behr ,  Aarthi Chandrasekaran ,  Ram Sasisekharan

    发明人： Scott Manalis ,  Tzu Loh ,  Michel Godin ,  Nebojsa Milovic ,  Jonathan Behr ,  Aarthi Chandrasekaran ,  Ram Sasisekharan

    IPC分类号： G01N33/86

    CPC分类号： C12Q1/56 ,  B01L3/5027 ,  G01N27/4145 ,  G01N2333/8128

    摘要： In one aspect, the present invention provides a device and method for real-time, direct detection of heparin in buffer and in serum comprising a microfluidic field-effect device as an affinity biosensor. The sensor is based on an electrolyte-insulator-silicon structure, and is manufactured by a standard high-yield silicon microfabrication process. The binding of heparin to the sensor surface induces a change in the insulator-electrolyte surface potential, which is measured as a change in sensor capacitance. To ensure the binding selectivity, protamine and antithrombin III are used as affinity probes.

    摘要翻译： 一方面，本发明提供了用于在缓冲液和血清中实时，直接检测肝素的装置和方法，其包括作为亲和生物传感器的微流场效应装置。 该传感器基于电解质 - 绝缘体 - 硅结构，并通过标准的高产率硅微细加工工艺制造。 肝素与传感器表面的结合导致绝缘体 - 电解质表面电位的变化，其被测量为传感器电容的变化。 为了确保结合选择性，使用鱼精蛋白和抗凝血酶III作为亲和探针。