Xylanase mutant and its preparation method and application

    公开(公告)号:US11447761B2

    公开(公告)日:2022-09-20

    申请号:US17219550

    申请日:2021-03-31

    IPC分类号: C12N9/24 A23K20/189 C12P19/14

    摘要: The present invention discloses a kind of xylanase mutant and its preparation method and application, which relates to the technical field of genomic engineering and genetic engineering. Such mutant includes one or more mutants obtained by taking xylanase HwXy110A as female parent to conduct saturation mutagenesis to the site of Gly363. Specifically, relates to obtaining 19 mutants through site-directed mutagenesis, and then conducting yeast expression to them, after that, obtaining two mutants with significantly improved specific activity and thermal stability through screening of thermal stability and catalytic activity; the present invention can significantly improve the thermal stability and catalytic efficiency of xylanase through modifying the site of Gly363, and is of important guiding significance for improving the thermal stability and the catalytic efficiency of the 10th family of xylanases and other glycoside hydrolases as well as lays the foundation for its application in industrial production.

    METHOD AND SYSTEM FOR PRODUCING A HIGH PROTEIN MEAL FROM A WHOLE STILLAGE BYPRODUCT AND SYSTEM THEREFOR

    公开(公告)号:US20220287332A1

    公开(公告)日:2022-09-15

    申请号:US17197520

    申请日:2021-03-10

    摘要: A method and system for producing a high protein meal from a whole stillage byproduct produced in a grain dry-milling process for making ethanol, with optional back end milling options, is disclosed. In one embodiment, a method for producing a high protein meal from a whole stillage byproduct includes separating the whole stillage byproduct into an insoluble solids portion and a thin stillage/centrate portion, wherein at least one milling step can be applied to one or both of the insoluble solids portion and the whole stillage portion. Next, the centrate portion may be separated into a protein portion and a water-soluble solids portion. And then the protein portion can be dewatered and/or dried to define a high protein meal having at least 40 wt % protein on a dry basis.

    PROCESS FOR THE PRODUCTION OF A MEAT ANALOGUE, AND MEAT ANALOGUE PREPARED THEREBY

    公开(公告)号:US20220287331A1

    公开(公告)日:2022-09-15

    申请号:US17636749

    申请日:2020-08-26

    摘要: Processes for the production of a meat analogue, comprising: a) introducing a meat batter comprising protein into a heating unit and heating the meat batter to a temperature above the melting temperature of the protein to produce a heat-treated product; b) transferring the heat-treated product to a cooling unit and cooling the heat-treated product to a temperature below water boiling an temperature at ambient pressure when exiting the cooling unit; and c) dividing the cooled heat-treated product into pieces, in which the pieces are at least partly re-introduced into the process; as well as a meat analogue prepared thereby.

    De-esterification of biomass prior to ammonia pretreatment

    公开(公告)号:US11440999B2

    公开(公告)日:2022-09-13

    申请号:US16029452

    申请日:2018-07-06

    摘要: A method is provided for pretreating cellulosic or lignocellulosic biomass comprising de-esterifying the biomass with an alkali treatment to produce de-esterified biomass; and pretreating the de-esterified biomass with ammonia to produce de-esterified ammonia pretreated biomass. In various embodiments, the de-esterified ammonia pretreated biomass has an amide concentration ranging from about 0.04 to about 25 mg/g biomass. In other embodiments, the amide concentration is no more than 0.04 mg/g biomass. Various ammonia pretreatment processes may be used, including liquid or gaseous ammonia pretreatments, including, but not limited to, liquid ammonium hydroxide pretreatments, various AFEX pretreatments, with or without biomass densification, and various cellulosic conversion pretreatments including “COBRA” pretreatments. Products (e.g., animal feed) and systems are also disclosed.