公开(公告)号：US07625757B2

公开(公告)日：2009-12-01

申请号：US11650962

申请日：2007-01-09

申请人： Tomohiro Tsuji ,  Ayumu Yoshida ,  Shinichiro Oguni

发明人： Tomohiro Tsuji ,  Ayumu Yoshida ,  Shinichiro Oguni

IPC分类号： G01N31/00 ,  G01N33/48

CPC分类号： G01N33/5094 ,  G01N15/1468 ,  G01N2015/008 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25125

摘要： A reagent for analyzing immature leukocyte capable of analyzing accurately immature leukocyte such as myeloblast and immature granulocyte, and mature leukocyte, which reagent has broader allowable range of sample processing conditions than conventional ones, and a reagent kit are provided. The present invention relates to a reagent for analyzing immature leukocyte containing a surfactant for giving damage to cell membrane of red blood cell and mature leukocyte in the sample, a solubilizing agent for causing contraction to damaged blood cell, sugar, a dye for staining nucleic acid.

摘要翻译： 提供能够分析准确地未成熟白细胞例如成髓细胞和未成熟粒细胞的未成熟白细胞和成熟白细胞的试剂，该试剂比常规试剂处理条件更宽的允许范围和试剂盒。 本发明涉及一种用于分析含有表面活性剂的未成熟白细胞用于对样品中红细胞和成熟白细胞的细胞膜造成损伤的试剂，用于引起血细胞损伤的增溶剂，糖，用于染色核酸的染料 。

公开(公告)号：US20090279770A1

公开(公告)日：2009-11-12

申请号：US12498533

申请日：2009-07-07

申请人： Albert E. Weller, III

发明人： Albert E. Weller, III

IPC分类号： G06K9/00

CPC分类号： G01N33/5094 ,  B01L9/06 ,  B01L2200/025 ,  G02B21/002 ,  G02B21/06 ,  G02B21/248 ,  G02B21/365 ,  G02B27/095 ,  G02B27/48

摘要： Fluorescent dye-tagged rare cells are disposed in a biological fluid layer contained in an annular gap (12) between an inside test tube wall (14) and a float wall (16). One or more analysis images are acquired at one or more focus depths using a microscope system (10, 10′, 10″, 10′″). Each acquired analysis image is image processed each to identify candidate rare cell images. The test tube (72) is selectively rotated. The test tube and the microscope system are selectively relatively translated along a test tube axis (75). The acquiring and image processing are repeated for a plurality of fields of view accessed by the selective rotating and selective relative translating and substantially spanning the annular gap.

摘要翻译： 将荧光染料标记的稀有细胞设置在包含在内部测试管壁（14）和浮动壁（16）之间的环形间隙（12）中的生物流体层中。 使用显微镜系统（10,10'，10“，10”）在一个或多个聚焦深度处获取一个或多个分析图像。 每个获取的分析图像被图像处理以识别候选稀有细胞图像。 试管（72）选择性旋转。 试管和显微镜系统沿着试管轴线（75）选择性地相对平移。 对于通过选择性旋转和选择性相对平移访问的多个视野并且基本跨越环形间隙，重复获取和图像处理。

公开(公告)号：US07560277B2

公开(公告)日：2009-07-14

申请号：US11519533

申请日：2006-09-12

申请人： Albert E. Weller, III

发明人： Albert E. Weller, III

IPC分类号： C12N5/00

CPC分类号： G01N33/5094 ,  B01L9/06 ,  B01L2200/025 ,  G02B21/002 ,  G02B21/06 ,  G02B21/248 ,  G02B21/365 ,  G02B27/095 ,  G02B27/48

摘要： Fluorescent dye-tagged rare cells are disposed in a biological fluid layer contained in an annular gap (12) between an inside test tube wall (14) and a float wall (16). One or more analysis images are acquired at one or more focus depths using a microscope system (10, 10′, 10″, 10′″). Each acquired analysis image is image processed each to identify candidate rare cell images. The test tube (72) is selectively rotated. The test tube and the microscope system are selectively relatively translated along a test tube axis (75). The acquiring and image processing are repeated for a plurality of fields of view accessed by the selective rotating and selective relative translating and substantially spanning the annular gap.

摘要翻译： 将荧光染料标记的稀有细胞设置在包含在内部测试管壁（14）和浮动壁（16）之间的环形间隙（12）中的生物流体层中。 使用显微镜系统（10,10'，10“，10”）在一个或多个聚焦深度处获取一个或多个分析图像。 每个获取的分析图像被图像处理以识别候选稀有细胞图像。 试管（72）选择性旋转。 试管和显微镜系统沿着试管轴线（75）选择性地相对平移。 对于通过选择性旋转和选择性相对平移访问的多个视野并且基本跨越环形间隙，重复获取和图像处理。

公开(公告)号：US20090148873A1

公开(公告)日：2009-06-11

申请号：US11975894

申请日：2007-10-22

申请人： Enrique Saldivar ,  Jennifer Orje

发明人： Enrique Saldivar ,  Jennifer Orje

IPC分类号： C12Q1/56

CPC分类号： G01N33/5094

摘要： An apparatus and method for measuring blood platelet contractility, hereinafter called a “retractometer” is disclosed. Also disclosed is a system apparatus and method for automatically measuring platelet contractility in a plurality of samples, having an array of retractometer units and an electronic solenoid valve controller to fully automate screening in clinical and research situations and.save costs in labor.

摘要翻译： 公开了一种用于测量血小板收缩力的装置和方法，以下称为“收缩仪”。 还公开了一种用于在多个样本中自动测量血小板收缩力的系统装置和方法，具有收缩计单元阵列和电子电磁阀控制器，以在临床和研究情况下完全自动化筛选，并降低劳动成本。

公开(公告)号：US20090142744A1

公开(公告)日：2009-06-04

申请号：US11887241

申请日：2006-03-22

申请人： Henri Champseix ,  Jerome Giraud

发明人： Henri Champseix ,  Jerome Giraud

IPC分类号： A01N1/02 ,  G01N33/00 ,  C12M1/00

CPC分类号： G01N33/721 ,  G01N15/1404 ,  G01N15/1459 ,  G01N33/5094 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1486 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25625

摘要： Method for the automatic analysis of a blood sample in which: an analysis solution containing the blood sample, a diluent, and at least one compound to lyze the erythrocytes; at least one compound to stabilize the haemoglobin in the form of a chromogenic complex, is formed in a single dilution and analysis tank, the haemoglobin level is measured in this analysis solution by spectrophotometry in the tank after the lysis of the erythrocytes; and an appropriate quantity of this analysis solution is taken from the tank on which a leucocytic differentiation is carried out by an optical elements characterized in that the analysis solution also contains at least one compound to protect the leucocytes, allowing the distinguishing of at least four main leucocyte sub-populations. A haematological analysis apparatus for the implementation of such a method is disclosed.

摘要翻译： 血液样本的自动分析方法，其中：含有血液样本，稀释剂和至少一种化合物的分析溶液以溶解红细胞; 在单个稀释和分析槽中形成至少一种稳定化合物形式的血红蛋白的化合物，在红细胞裂解后，通过分光光度法在该分析溶液中测量血红蛋白水平; 并且从其中通过光学元件进行白细胞分化的罐获取适量的该分析溶液，其特征在于分析溶液还含有至少一种保护白细胞的化合物，允许区分至少四个主要 白细胞亚群。 公开了一种用于实施这种方法的血液分析装置。

公开(公告)号：US20090093014A1

公开(公告)日：2009-04-09

申请号：US12157937

申请日：2008-06-12

申请人： Michael Burnet ,  Jan-Hinrich Guse ,  Gene Kim ,  Albert Beck ,  Georgia Tsotsou ,  Irina Droste-Borel ,  Laurence Barker ,  Michael Wolff ,  Hans-Jurgen Gutke

发明人： Michael Burnet ,  Jan-Hinrich Guse ,  Gene Kim ,  Albert Beck ,  Georgia Tsotsou ,  Irina Droste-Borel ,  Laurence Barker ,  Michael Wolff ,  Hans-Jurgen Gutke

IPC分类号： C12Q1/02 ,  C12N5/06

CPC分类号： B82Y5/00 ,  A61K47/552 ,  A61K47/556 ,  A61K47/67 ,  A61K49/0004 ,  G01N33/5047 ,  G01N33/5094

摘要： This invention features a method of identifying a compound useful for enhancing efficacy of a therapeutic agent. The method includes incubating a compound in blood cells; separating immune cells from erythrocytic cells; and determining the ratio of the concentration of the compound in the immune cells to the concentration of the compound in the erythrocytic cells; wherein the compound comprises a transportophore and a therapeutic agent, in which the transportophore is covalently bonded to the therapeutic agent via a bond or a linker.

摘要翻译： 本发明的特征在于鉴定可用于增强治疗剂功效的化合物的方法。 该方法包括在血细胞中孵育化合物; 将免疫细胞与红细胞分离; 并测定免疫细胞中化合物的浓度与红细胞中化合物浓度之比; 其中所述化合物包含转运载体和治疗剂，其中所述转运载体通过键或接头共价键合到所述治疗剂。

公开(公告)号：US20080266658A1

公开(公告)日：2008-10-30

申请号：US12168373

申请日：2008-07-07

申请人： John S. Laudo

发明人： John S. Laudo

IPC分类号： G02B21/06 ,  G02B27/09

CPC分类号： G01N33/5094 ,  B01L9/06 ,  B01L2200/025 ,  G02B21/002 ,  G02B21/06 ,  G02B21/248 ,  G02B21/365 ,  G02B27/095 ,  G02B27/48

摘要： A microscope system (10, 10′, 10″, 10′″) includes a laser (18) or light emitting diode (18″′) that generates source light having a non-uniform spatial distribution. An optical system includes an objective (40) defining a field of view, and an optical train (22, 22′, 22″, 22″′) configured to convert the source light into an enlarged-diameter collimated light, to spatially homogenize the enlarged-diameter collimated light, and to couple the homogenized enlarged-diameter collimated light into the objective to provide substantially uniform static illumination of the field of view. A camera system (56) is statically optically coupled by the objective with at least most of the field of view.

摘要翻译： 显微镜系统（10,10'，10“，10”）包括产生具有不均匀空间分布的源光的激光器（18）或发光二极管（18“）。 光学系统包括限定视野的物镜（40）和被配置为将源光转换成放大直径的准直光的光学系统（22,22'，22“，22”'） 均匀化扩大直径的准直光，并将均匀化的扩大直径的准直光耦合到物镜中，以提供视野的基本上均匀的静态照明。 相机系统（56）通过至少大部分视野被物镜静态光耦合。

公开(公告)号：US07425421B2

公开(公告)日：2008-09-16

申请号：US10878456

申请日：2004-06-28

申请人： Stephen D. Dertinger

发明人： Stephen D. Dertinger

IPC分类号： G01N33/00

CPC分类号： G01N33/56966 ,  G01N33/5094 ,  G01N33/80 ,  G01N2333/70582 ,  G01N2333/70596 ,  Y02A50/58 ,  Y10S435/968 ,  Y10S435/973 ,  Y10T436/101666

摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.

摘要翻译： 用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体 ，以及在红细胞群体中染色DNA（微核）的核酸染色染料。 因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射 由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。 特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

公开(公告)号：US07413905B2

公开(公告)日：2008-08-19

申请号：US11517031

申请日：2006-09-07

申请人： Wenjuan Xu ,  Lina Zhang ,  Bing Liu

发明人： Wenjuan Xu ,  Lina Zhang ,  Bing Liu

IPC分类号： G01N31/00 ,  G01N33/48

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： The present invention relates to a reagent for classifying leukocytes including (a) at least one surfactant capable of lysing erythrocytes and partly damaging the cell membrane of leukocytes, (b) at least one organic compound bearing an anionic group capable of binding to the cationic component present in the leukocytes to provide morphological differences between the leukocytes, and (c) a buffer for adjusting pH to 2˜8. Also disclosed is a method for classifying leukocytes into four groups.

摘要翻译： 本发明涉及一种用于分类白细胞的试剂，其包括（a）至少一种能够溶解红细胞并部分损伤白细胞细胞膜的表面活性剂，（b）至少一种带有能够结合阳离子成分的阴离子基团的有机化合物 存在于白细胞中以提供白细胞之间的形态学差异，和（c）用于将pH调节至2〜8的缓冲液。 还公开了将白细胞分类为四组的方法。

公开(公告)号：US20080187951A1

公开(公告)日：2008-08-07

申请号：US12023501

申请日：2008-01-31

申请人： Takaaki NAGAI ,  Noriyuki Narisada ,  Hans Kalkman

发明人： Takaaki NAGAI ,  Noriyuki Narisada ,  Hans Kalkman

IPC分类号： C12Q1/20 ,  C12M1/34

CPC分类号： G01N33/5094 ,  G01N15/12 ,  G01N15/1459 ,  G01N33/721 ,  G01N33/80 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/1402 ,  G01N2015/1486

摘要： A hematological analyzer for measuring blood, sets a body fluid measurement mode; receives a measurement start instruction; irradiates a measurement sample with light and obtains optical information from cells contained in the measurement sample; and classifies at least white blood cells and nucleated cells other than white blood cells contained in the measurement sample, and counts the white blood cells and nucleated cells other than white blood cells based on the optical information obtained from the cells in the measurement sample prepared from a body fluid sample and white blood cell measuring reagent when the body fluid measurement mode has been set and the measurement start instruction has been selected, is disclosed. A method for analyzing body fluid and a computer program product are also disclosed.

摘要翻译： 用于测量血液的血液分析仪，设定体液测量模式; 接收测量开始指令; 用光照射测量样品，并从测量样品中包含的细胞获得光学信息; 并且至少对除了测量样品中包含的白细胞以外的白细胞和有核细胞进行分类，并且基于从由从细胞中制备的测量样品中的细胞获得的光学信息对白血细胞以外的白细胞和有核细胞进行计数 公开了当已经设置体液测量模式并且已经选择测量开始指令时的体液样品和白细胞测量试剂。 还公开了一种用于分析体液的方法和计算机程序产品。