公开(公告)号：US20230324383A1

公开(公告)日：2023-10-12

申请号：US17989895

申请日：2022-11-18

申请人： KELLBENX INCORPORATED

发明人： Christiane HOLLMANN ,  Silke ZIMMERMANN ,  Stefan STACHELHAUS ,  Winfried ALBERT

IPC分类号： G01N33/569 ,  G01N33/50 ,  G01N33/577 ,  G01N33/80 ,  C07K16/28

CPC分类号： G01N33/56966 ,  G01N33/5094 ,  G01N33/577 ,  G01N33/80 ,  C07K16/28 ,  Y10T436/25375 ,  Y10T436/25125 ,  C07K2317/14 ,  G01N2333/70582 ,  G01N2469/10

摘要： The present invention concerns a monoclonal antibody and corresponding hybridoma cells and antigens suitable for isolating fetal cells from maternal blood. The inventive monoclonal antibody reacts with a surface antigen present on fetal red blood cells including their nucleated precursor cells, but not with surface antigens on adult erythroid cell.

公开(公告)号：US20190240290A1

公开(公告)日：2019-08-08

申请号：US16318512

申请日：2017-08-04

申请人： H. Lee Moffitt Cancer Center and Research Institute, Inc.

发明人： Alan F. List

IPC分类号： A61K38/18 ,  A61K31/454 ,  G01N33/569 ,  C12Q1/6883 ,  A61P7/06

CPC分类号： A61K38/1816 ,  A61K31/454 ,  A61P7/06 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/158 ,  G01N33/56966 ,  G01N33/57407 ,  G01N2333/70582 ,  G01N2333/70589 ,  G01N2800/52 ,  A61K2300/00

摘要： Disclosed herein is a companion diagnostic to predict efficacy of combination lenalidomide and erythropoietin treatment in patients with a erythropoietin (Epo)-refractory, Lower Risk (LR) Non-deletion 5q [Del(5q)] myelodysplasia syndrome (MDS). The method involves assaying erythroid precursors from a biological sample from the subject for a CD45 isoform profile, and treating the subject with a combination of lenalidomide and erythropoietin if the erythroid precursors have a predominance of large CD45RA and CD45RB isoforms compared to small CD45RO isoform.

公开(公告)号：US08889369B2

公开(公告)日：2014-11-18

申请号：US14075082

申请日：2013-11-08

申请人： Litron Laboratories, Ltd.

发明人： Stephen D. Dertinger

IPC分类号： G01N33/00 ,  G01N33/50 ,  G01N33/80

CPC分类号： G01N33/56966 ,  G01N33/5094 ,  G01N33/80 ,  G01N2333/70582 ,  G01N2333/70596 ,  Y02A50/58 ,  Y10S435/968 ,  Y10S435/973 ,  Y10T436/101666

摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.

摘要翻译： 用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体 ，以及在红细胞群体中染色DNA（微核）的核酸染色染料。 因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射 由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。 特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

公开(公告)号：US20130260459A1

公开(公告)日：2013-10-03

申请号：US13896234

申请日：2013-05-16

申请人： Michael Coleman ,  Jody Vykoukal

发明人： Michael Coleman ,  Jody Vykoukal

IPC分类号： C12N5/09 ,  C12N5/071

CPC分类号： C12N5/0693 ,  A01N1/0278 ,  C12N5/0667 ,  G01N1/40 ,  G01N1/42 ,  G01N33/5047 ,  G01N2333/42 ,  G01N2333/70582 ,  G01N2333/70589 ,  G01N2333/70596

摘要： Methods for obtaining and preserving target cells using degradable three dimensional matrices are described.

摘要翻译： 描述了使用可降解三维矩阵获得和保存靶细胞的方法。

公开(公告)号：US20120107803A1

公开(公告)日：2012-05-03

申请号：US13289764

申请日：2011-11-04

申请人： Stephen D. Dertinger

发明人： Stephen D. Dertinger

IPC分类号： C12Q1/68

CPC分类号： G01N33/56966 ,  G01N33/5094 ,  G01N33/80 ,  G01N2333/70582 ,  G01N2333/70596 ,  Y02A50/58 ,  Y10S435/968 ,  Y10S435/973 ,  Y10T436/101666

摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.

摘要翻译： 用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体 ，以及在红细胞群体中染色DNA（微核）的核酸染色染料。 因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射 由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。 特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

公开(公告)号：US20110076688A1

公开(公告)日：2011-03-31

申请号：US12962788

申请日：2010-12-08

申请人： Stephen D. Dertinger

发明人： Stephen D. Dertinger

IPC分类号： C12Q1/68

CPC分类号： G01N33/56966 ,  G01N33/5094 ,  G01N33/80 ,  G01N2333/70582 ,  G01N2333/70596 ,  Y02A50/58 ,  Y10S435/968 ,  Y10S435/973 ,  Y10T436/101666

摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.

摘要翻译： 用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体 ，以及在红细胞群体中染色DNA（微核）的核酸染色染料。 因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射 由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。 特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

公开(公告)号：US20080293072A1

公开(公告)日：2008-11-27

申请号：US12177352

申请日：2008-07-22

申请人： Stephen D. Dertinger

发明人： Stephen D. Dertinger

IPC分类号： C12Q1/68

CPC分类号： G01N33/56966 ,  G01N33/5094 ,  G01N33/80 ,  G01N2333/70582 ,  G01N2333/70596 ,  Y02A50/58 ,  Y10S435/968 ,  Y10S435/973 ,  Y10T436/101666

摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.

摘要翻译： 用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体 ，以及在红细胞群体中染色DNA（微核）的核酸染色染料。 因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射 由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。 特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

公开(公告)号：US20070274919A1

公开(公告)日：2007-11-29

申请号：US11752726

申请日：2007-05-23

申请人： Stephen D. Dertinger

发明人： Stephen D. Dertinger

IPC分类号： A61K49/00 ,  C12Q1/00 ,  G01N33/567

CPC分类号： G01N33/80 ,  G01N2333/70557 ,  G01N2333/70582 ,  G01N2333/70596 ,  Y10S435/973 ,  Y10S436/811 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/143333 ,  Y10T436/25125

摘要： The present invention relates a method for the enumeration of in vivo gene mutation. The method utilizes differential staining of GPI-anchor deficient erythrocyte populations to distinguish between wild-type and pig-a gene mutants. Quantitative analyses can be conducted on erythrocytes and/or reticulocytes, and is based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of mutant erythrocytes or reticulcoytes relative to the number of total erythrocytes or reticulocytes can be used to assess the DNA-damaging potential of an exogenous chemical agent, the DNA-damaging potential of an exogenous physical agent, the effects of an exogenous agent which can modify endogenously-induced DNA damage, and the effects of an exogenous agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.

摘要翻译： 本发明涉及一种枚举体内基因突变的方法。 该方法利用GPI锚定缺陷型红细胞群体的差异染色来区分野生型和pig-a基因突变体。 可以对红细胞和/或网织红细胞进行定量分析，并且基于暴露于兴奋性光源之后的荧光发射和光散射。 可以使用相对于总红细胞或网织红细胞数的突变型红细胞或网状细胞计数来评估外源性化学试剂的DNA损伤潜力，外源物理试剂的DNA损伤潜能，外源性试剂的作用 改变内源性诱导的DNA损伤，以及可以改变外源诱导的DNA损伤的外源药物的作用。 还公开了用于实施本发明的试剂盒。

公开(公告)号：US6156882A

公开(公告)日：2000-12-05

申请号：US105596

申请日：1998-06-26

申请人： Hans-Jorg Buhring ,  Irene Rappold

发明人： Hans-Jorg Buhring ,  Irene Rappold

IPC分类号： A61K38/00 ,  C07K14/705 ,  C07K16/28 ,  C12N15/06 ,  C12P21/08 ,  G01N33/569 ,  C12N9/64 ,  C07K16/00

CPC分类号： G01N33/56972 ,  C07K14/70596 ,  C07K16/2896 ,  A61K38/00 ,  G01N2333/70582 ,  G01N2333/71

摘要： The invention relates to a monoclonal antibody specifically binding to the human FLT3/FLK2 receptor protein. The invention further relates to hybridoma cells producing such an antibody, as well as to a method for generation of such hybridoma cells. The monoclonal antibody is the antibody produced and released by hybridoma cells deposited under No. DSM 2249 at the German Collection of Microorganisms and Cell Cultures (DSMZ) and designated as 4G8B4B12.

摘要翻译： 本发明涉及与人FLT3 / FLK2受体蛋白特异性结合的单克隆抗体。 本发明还涉及产生这种抗体的杂交瘤细胞，以及用于产生这种杂交瘤细胞的方法。 单克隆抗体是由DSM 2249在德国微生物和细胞培养物收集库（DSMZ）上保藏并被指定为4G8B4B12的杂交瘤细胞产生和释放的抗体。

公开(公告)号：US20180057604A1

公开(公告)日：2018-03-01

申请号：US15525821

申请日：2015-11-18

申请人： Genentech, Inc. ,  Adimab LLC

发明人： Yichin Liu ,  Jasvinder Atwal ,  Cecilia Pui Chi Chiu ,  Ryan J. Watts ,  Yan WU ,  Eric Krauland ,  Michael Feldhaus ,  Yin Zhang ,  Joy Yu Zuchero ,  Jessica Couch ,  Mark S Dennis ,  James A Ernst ,  Gregory A Lazar

IPC分类号： C07K16/40 ,  C07K16/28 ,  G01N33/68

CPC分类号： C07K16/40 ,  A61K2039/505 ,  C07K16/22 ,  C07K16/2881 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/528 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/71 ,  C07K2317/72 ,  C07K2317/732 ,  C07K2317/76 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2317/94 ,  C07K2319/00 ,  C12Y304/23046 ,  G01N33/6896 ,  G01N2333/70582 ,  G01N2333/96472 ,  G01N2800/28 ,  G01N2800/52

摘要： The present invention relates to bispecific antibodies that bind to transferrin receptor and BACE1 and methods of using the same.