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    Alpha-Amylase Variants
    121.
    发明申请
    Alpha-Amylase Variants 有权
    α-淀粉酶变体

    公开(公告)号：US20120252095A1

    公开(公告)日：2012-10-04

    申请号：US13478512

    申请日：2012-05-23

    申请人： Allan Svendsen ,  Sóeren Kjaerulff ,  Henrik Bisgaard-Frantzen ,  Carsten Andersen

    发明人： Allan Svendsen ,  Sóeren Kjaerulff ,  Henrik Bisgaard-Frantzen ,  Carsten Andersen

    IPC分类号： C12N9/28

    CPC分类号： C12N9/2414 ,  C07H21/04 ,  C11D3/38609 ,  C11D3/38618 ,  C12N9/2417

    摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.

    摘要翻译： 本发明涉及亲本的类戊酰样α-淀粉酶的变体，其包含2,3,4,5或6个/位置的突变。 这些变体在高温下（相对于亲本）具有增加的稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体表现出增加。

    CREATION OF DIVERSITY IN POLYPEPTIDES
    124.
    发明申请
    CREATION OF DIVERSITY IN POLYPEPTIDES 失效
    多糖生物多样性的创造

    公开(公告)号：US20110287142A1

    公开(公告)日：2011-11-24

    申请号：US13197167

    申请日：2011-08-03

    申请人： Allan Svendsen ,  Lars Beier

    发明人： Allan Svendsen ,  Lars Beier

    IPC分类号： A21D8/04 ,  C07H21/04 ,  C12N9/26

    CPC分类号： A21D8/042 ,  C12N9/1048 ,  C12N9/2417 ,  C12N15/1027 ,  C12N15/1058 ,  C12N15/1089

    摘要： The inventors realized that the diversity generated by conventional methods may be limited by steric hindrance between amino acid residues in the three-dimensional structures of the resulting polypeptides. The steric hindrance may occur between amino acid residues at widely different positions in the amino acid sequences, e.g. between residues in two different domains of the 3D structure, and resulting polypeptides which include such steric hindrance may never be observed in the conventional recombination methods because they may be expressed in poor yields or may have poor activity or stability.The inventors developed a method to identify and alleviate such steric hindrance in the resulting polypeptides. In an alignment of the three-dimensional structures, steric hindrance is indicated when residues from two different structures are located within a certain distance. Pairs of residues at corresponding positions in the amino acid sequences are not considered, and residues close to the surface (high solvent accessibility) are considered to be less prone to steric hindrance.

    摘要翻译： 发明人意识到，通过常规方法产生的多样性可能受到所得多肽的三维结构中的氨基酸残基之间的空间位阻的限制。 空间位阻可以发生在氨基酸序列中广泛不同位置的氨基酸残基之间，例如， 在3D结构的两个不同结构域中的残基之间以及包含这种空间位阻的所得多肽在常规重组方法中可能永远不会观察到，因为它们可能以不良产率表达或可能具有差的活性或稳定性。 发明人开发了鉴定和减轻所得多肽中这种空间位阻的方法。 在三维结构的排列中，当来自两个不同结构的残基位于一定距离内时，指示空间位阻。 不考虑氨基酸序列中对应位置的残基对，并且靠近表面的残留物（高溶剂可及性）被认为不太容易产生空间位阻。

    SUBTILASES
    127.
    发明申请
    SUBTILASES 有权

    公开(公告)号：US20110003364A1

    公开(公告)日：2011-01-06

    申请号：US12879410

    申请日：2010-09-10

    申请人： Allan Svendsen ,  Henriette Draborg

    发明人： Allan Svendsen ,  Henriette Draborg

    IPC分类号： C12N9/56 ,  C07H21/04 ,  C12N1/21

    CPC分类号： C12N9/54 ,  C11D3/386 ,  C12Y304/21062

    摘要： The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN′ subtilase and to TY145 and BPN′ variants having altered properties as compared to the parent TY145/BPN′ subtilase.

    摘要翻译： 本发明涉及用于产生亲本TY145脱落酶和亲本BPN'脱羧酶的变体的方法，并且与亲本TY145 / BPN'脱羧酶相比具有改变的性质的TY145和BPN'变体的方法。

    Alpha-Amylase Mutants
    129.
    发明申请
    Alpha-Amylase Mutants 失效
    α-淀粉酶突变体

    公开(公告)号：US20100144575A1

    公开(公告)日：2010-06-10

    申请号：US12100506

    申请日：2008-04-10

    申请人： Allan Svendsen ,  Henrik Bisgard-Frantzen ,  Torben Vedel Borchert

    发明人： Allan Svendsen ,  Henrik Bisgard-Frantzen ,  Torben Vedel Borchert

    IPC分类号： C12N9/28 ,  G01N33/53 ,  C12Q1/40 ,  C12N9/30 ,  C12N1/21 ,  C12N1/15 ,  C12N15/75 ,  C12N15/80 ,  C11D3/386 ,  C12N15/56

    CPC分类号： C11D3/386 ,  C12N9/2417

    摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like α-amylase, which variant has α-amylase activity and at least one altered property as compared to the parent α-amylase, comprises i) analysing the structure of the parent Termamyl-like α-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like α-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like α-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like α-amylase variant, which as compared to the parent Termamyl-like α-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like α-amylase variant with respect to the property in question.

    摘要翻译： 本发明涉及构建母体类戊酰样α-淀粉酶的变体的方法，该变体与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类戊酰样α-淀粉酶结构的至少一个结构部分，该氨基酸残基或结构部分被认为与改变 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变性质，以及任选地，iii）相对于所讨论的性质测试所得到的类戊酰样α-淀粉酶变体。

    Subtilases
    130.
    发明授权
    Subtilases 失效
    分散液

    公开(公告)号：US07727756B2

    公开(公告)日：2010-06-01

    申请号：US10807096

    申请日：2004-03-22

    申请人： Allan Svendsen ,  Stefan Minning

    发明人： Allan Svendsen ,  Stefan Minning

    IPC分类号： C12N9/52 ,  C12N15/57 ,  C12N15/70 ,  C12N15/74 ,  C12N15/75

    CPC分类号： C12N9/54 ,  C07K2299/00

    摘要： The present invention relates to methods for producing variants of a parent JP170 subtilase and of a parent BPN′ subtilase and to JP170 and BPN′ variants having altered properties as compared to the parent JP170/BPN′ subtilase.

    摘要翻译： 本发明涉及与亲本JP170 / BPN'脱羧酶相比，用于产生亲本JP170脱落酶和亲本BPN'脱羧酶的变体的方法和具有改变的性质的JP170和BPN'变体的方法。