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公开(公告)号:US08124395B2
公开(公告)日:2012-02-28
申请号:US12952695
申请日:2010-11-23
申请人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号: C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译: 本发明涉及母体类戊酰样α-淀粉酶的变体,该变体表现出改变的性质,特别是降低了切割接近分支点的底物的能力,以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。
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公开(公告)号:US20090155882A1
公开(公告)日:2009-06-18
申请号:US11934136
申请日:2007-11-02
申请人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号: C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译: 本发明涉及母体类戊酰样α-淀粉酶的变体,该变体表现出改变的性质,特别是降低了切割接近分支点的底物的能力,以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。
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公开(公告)号:US07306936B2
公开(公告)日:2007-12-11
申请号:US10146327
申请日:2002-05-15
申请人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号: C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译: 本发明涉及母体类戊酰样α-淀粉酶的变体,该变体表现出改变的性质,特别是降低了切割接近分支点的底物的能力,以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。
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公开(公告)号:US07867746B2
公开(公告)日:2011-01-11
申请号:US11934136
申请日:2007-11-02
申请人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号: C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译: 本发明涉及母体类戊酰样α-淀粉酶的变体,该变体表现出改变的性质,特别是降低了切割接近分支点的底物的能力,以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。
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公开(公告)号:US20110076741A1
公开(公告)日:2011-03-31
申请号:US12952695
申请日:2010-11-23
申请人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号: C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译: 本发明涉及母体类戊酰样α-淀粉酶的变体,该变体表现出改变的性质,特别是降低了切割接近分支点的底物的能力,以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。
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公开(公告)号:US06887986B1
公开(公告)日:2005-05-03
申请号:US09441313
申请日:1999-11-16
CPC分类号: C12N9/2414 , C07H21/04 , C11D3/38609 , C11D3/38618 , C12N9/2417
摘要: The invention relates to a variant of a parent Termamyl-like α-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an α-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an α-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an α-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an α-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like α-amylase, which variant exhibits increased.
摘要翻译: 本发明涉及亲本的类戊酰样α-淀粉酶的变体,其包含2,3,4,5或6个/位置的突变。 这些变体在高温下(相对于亲本)具有增加的稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体,携带本发明的DNA构建体的重组表达载体,用本发明的DNA构建体转化的细胞,使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤,纺织品退浆,淀粉液化,包含本发明的α-淀粉酶变体的洗涤剂添加剂,手动或自动洗碗洗涤剂组合物,其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法,该变体表现出增加。
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公开(公告)号:US08420370B2
公开(公告)日:2013-04-16
申请号:US13355047
申请日:2012-01-20
申请人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgaard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgaard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号: C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
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公开(公告)号:US08465957B2
公开(公告)日:2013-06-18
申请号:US13478512
申请日:2012-05-23
IPC分类号: C12N9/28
CPC分类号: C12N9/2414 , C07H21/04 , C11D3/38609 , C11D3/38618 , C12N9/2417
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.
摘要翻译: 本发明涉及亲本的类戊酰样α-淀粉酶的变体,其包含2,3,4,5或6个/位置的突变。 这些变体在高温下(相对于亲本)具有增加的稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体,携带本发明的DNA构建体的重组表达载体,用本发明的DNA构建体转化的细胞,使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤,纺织品退浆,淀粉液化,包含本发明的α-淀粉酶变体的洗涤剂添加剂,手动或自动洗碗洗涤剂组合物,其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法,该变体表现出增加。
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公开(公告)号:US20090263881A1
公开(公告)日:2009-10-22
申请号:US11953532
申请日:2007-12-10
申请人: Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
发明人: Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
IPC分类号: C12N9/26
摘要: The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译: 本发明涉及亲本的类戊酰样α-淀粉酶的变体,其相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变:i)在8至10.5的pH下改善的pH稳定性; 和/或ii)在pH8至10.5时提高的Ca 2+稳定性,和/或iii)在10至60℃的温度下提高比活性。
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公开(公告)号:US20050084937A1
公开(公告)日:2005-04-21
申请号:US10980923
申请日:2004-11-04
申请人: Torben Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Nissen , Soren Kjaerulff
发明人: Torben Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Nissen , Soren Kjaerulff
摘要: The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译: 本发明涉及亲本的类戊酰样α-淀粉酶的变体,其相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变:i)在8至10.5的pH下改善的pH稳定性; 和/或ii)在pH 8至10.5下改善的Ca 2+稳定性,和/或iii)在10至60℃的温度下提高比活性。
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