公开(公告)号：US20140080716A1

公开(公告)日：2014-03-20

申请号：US13972152

申请日：2013-08-21

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  Adrianus Johannes van Tunen

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2600/13 ,  G06F19/22 ,  C12Q2525/155 ,  C12Q2563/179 ,  C12Q2563/155 ,  C12Q2537/143

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

Abstract translation: 公开了有效的方法用于在诱变的群体成员中基因突变的高通量鉴定。 所述方法包括DNA分离，合并，扩增，文库的产生，文库的高通量测序，优选通过合成序列测序技术，鉴定突变和携带突变和识别突变的群体成员的鉴定。

公开(公告)号：US12236655B2

公开(公告)日：2025-02-25

申请号：US17771116

申请日：2020-10-29

Applicant: Keygene N.V.

Inventor： Marco Gerardus Maria van Schriek ,  Rudolf Laurentius van Bavel ,  Niek Bouman

IPC: G06V10/00 ,  B07C5/342 ,  G02B27/01 ,  G06F18/21 ,  G06V10/22

Abstract: A plant selection apparatus has a storage means for storing a plurality of image datasets of plants and associated plant information including at least one of genotype information of the plants, phenotype information of the plants, and pedigree information of the plants. A selection unit preselects a subset of the plants. A display device displays the image datasets of the subset of the plants. A tracking unit generates observation information including information on which of the subset of the plants is observed, and the storage means is configured for storing the observation information. A training unit trains a classifier based on the observation information, the input selection, and the plant information including said at least one of the genotype information, the phenotype information, and the pedigree information.

公开(公告)号：US12215363B2

公开(公告)日：2025-02-04

申请号：US16811393

申请日：2020-03-06

Applicant: Keygene N.V.

Inventor： Paul Bundock

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/82 ,  C12N15/90

Abstract: The invention pertains to the targeted alteration of a duplex DNA in a cell, whereby two site-specific nucleases generate an indel, such that the open reading frame is not altered after the second indel. The invention further pertains to the use of such nucleases for the targeted alteration of an open reading frame in duplex DNA and a kit of parts for use in a method of the invention. Using the method of the invention, novel plants were obtained having an improved herbicide resistance. The invention therefore also concerns plants having improved herbicide resistance due to the expression of an altered ALS protein.

公开(公告)号：US20240175040A1

公开(公告)日：2024-05-30

申请号：US18487029

申请日：2023-10-13

Applicant: Keygene N.V.

Inventor： Jeroen STUURMAN ,  Bipna Rani SHRESTHA ,  Hugo Ferdinand HOFHUIS

IPC: C12N15/82 ,  C12N9/22

CPC classification number: C12N15/8202 ,  C12N9/22 ,  C12N2310/20

Abstract: The invention concerns a method for producing a shoot of a plant comprising germline progenitor cells of a recalcitrant plant. The germline progenitor cells may be modified to comprise a mutation in a sequence of interest. The invention further pertains to plants obtainable by the method of the invention, wherein the plant preferably comprises at least the L2-meristem layer of the recalcitrant plant.

公开(公告)号：US20240150740A1

公开(公告)日：2024-05-09

申请号：US18487025

申请日：2023-10-13

Applicant: Keygene N.V.

Inventor： Julia Johanna WIND ,  Bjorn Alexander KLOOSTERMAN ,  Franck Georges Paul LHUISSIER

IPC: C12N9/22 ,  C12N15/74 ,  C12N15/82 ,  C12N15/86

CPC classification number: C12N9/22 ,  C12N15/743 ,  C12N15/8229 ,  C12N15/86 ,  C12N2310/20 ,  C12N2770/00043

Abstract: The invention concerns the targeted genomic modification of a plant cell, preferably a meristem cell. More in particular, the invention pertains to a vector expressing a coding RNA, wherein the coding RNA comprises a sequence encoding a CRISPR-nuclease and a mobile element, wherein the mobile element enables intercellular translocation of the coding RNA, preferably intercellular translocation to a meristem cell. The invention further concerns an editing RNA comprising the coding RNA and further comprising a guide RNA.

公开(公告)号：US11950554B2

公开(公告)日：2024-04-09

申请号：US17984938

申请日：2022-11-10

Applicant: Keygene N.V.

Inventor： Diana Rigola ,  René Johannes Maria Hofstede ,  Magdalena Barbara Lastdrager ,  Jamila Maguy Chaïb

IPC: A01H1/04 ,  A01H5/08 ,  C12N15/82 ,  C12Q1/6895

CPC classification number: A01H1/045 ,  A01H5/08 ,  C12N15/8282 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: The present invention relates to genetic elements comprising powdery mildew-conferring QTLs derived from a plant of the species Cucumis melo, or powdery mildew-conferring part or variant thereof. The invention also relates to markers for identification of said QTLs, use thereof and methods for producing plants with increased resistance to powdery mildew and the plants thus obtained.

公开(公告)号：US11873529B2

公开(公告)日：2024-01-16

申请号：US17208728

申请日：2021-03-22

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van Eijk ,  Rene Cornelis Josephus Hogers

IPC: C12Q1/6827 ,  C12Q1/6874 ,  C12Q1/683

CPC classification number: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6827 ,  C12Q1/6827 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2561/125 ,  C12Q1/6827 ,  C12Q2525/131 ,  C12Q2525/155 ,  C12Q2561/125

Abstract: The invention relates to a kit of parts for detecting one or more polymorphisms in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises (i) a first probe and a second probe, wherein the first probe comprises a first target specific section and a first tag section that is non-complementary to the target nucleotide sequence and that comprises a first universal primer binding site, wherein the second probe comprises a second target specific section and a second tag section that is non-complementary to the target nucleotide sequence; and (ii) a first primer and optionally a second primer wherein the first primer comprises a sample-specific identifier sequence and wherein the first primer can hybridize to the first universal primer binding site.

公开(公告)号：US11576318B2

公开(公告)日：2023-02-14

申请号：US17106463

申请日：2020-11-30

Applicant: Keygene N.V.

Inventor： Rik Hubertus Martinus Op Den Camp ,  Peter Johannes Van Dijk ,  Anthony Gallard

IPC: A01H1/08 ,  C07K14/415 ,  C12N15/82

Abstract: It was found that plants with loss of functional Msi2 protein due to a nucleotide polymorphism resulting in the introduction of a premature stop codon in the Msi2 protein, are able to induce haploid offspring after a cross to or with a wild type plant comprising a functional Msi2 protein. The invention relates to generation of haploid and doubled haploid plants.

公开(公告)号：US11371051B2

公开(公告)日：2022-06-28

申请号：US16310764

申请日：2017-06-20

Applicant: Keygene N.V.

Inventor： Paul Bundock ,  Anita Ketelaars-Bonné

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed is a new method of providing plant cells with a targeted alteration in a DNA molecule. The method comprises contacting a population of plant cells comprising a DNA molecule, the DNA molecule having a target sequence, with an aqueous medium, wherein the aqueous medium comprises a CRISPR associated protein (CAS protein) or a CAS-like protein, and a CRISPR-Cas system guide RNA that hybridizes with the target sequence, and wherein the aqueous medium comprises polyethylene glycol (PEG), but needs to be substantially free of glycerol.

公开(公告)号：US20220186238A1

公开(公告)日：2022-06-16

申请号：US17559924

申请日：2021-12-22

Applicant: KEYGENE N.V.

Inventor： Peter Johannes VAN DIJK ,  Diana RIGOLA ,  Marinus Willem PRINS ,  Adrianus Johannes VAN TUNEN

IPC: C12N15/82 ,  C12Q1/68 ,  C12Q1/6895 ,  C12N15/113 ,  C12N15/62

Abstract: The invention provides nucleotide sequences and amino acid sequences of the Dip gene as well as (functional) homologues, fragments and variants thereof, which provides diplospory as a part of apomixis. Also diplospory plants and methods for making these are provided, as are methods of using these, and methods of making apomictic seed.