公开(公告)号：US20110162963A1

公开(公告)日：2011-07-07

申请号：US12999157

申请日：2009-06-18

Applicant: Andrew D Hibbs ,  Geoffrey Alden Barrall ,  Eric N Ervin ,  Daniel K Lathrop

Inventor： Andrew D Hibbs ,  Geoffrey Alden Barrall ,  Eric N Ervin ,  Daniel K Lathrop

IPC: G01N27/447

CPC classification number: G01N33/48721

Abstract: An electrolytic system includes an analyte chamber having an access port for introducing a sample containing a molecules of interest, such as DNA. Electrodes create an electric field along a length of the analyte chamber to drive molecules toward an interaction region containing a nanopore, thereby increasing the arrival rate of molecules at the nanopore. Additional electrodes may be utilized to create an electric field through the nanopore to drive a molecule into the nanopore. A current sensor may be utilized to count, discriminate or characterize the molecules as they interact with the nanopore. Advantageously, system can be utilized for unamplified DNA sequencing.

Abstract translation: 电解系统包括分析物室，其具有用于引入含有目标分子（例如DNA）的样品的入口。 电极沿分析物室的长度产生电场，以将分子驱向包含纳米孔的相互作用区域，从而增加分子在纳米孔处的到达速率。 可以使用附加的电极来产生通过纳米孔的电场以将分子驱动到纳米孔中。 当电流传感器与纳米孔相互作用时，可以使用电流传感器对分子进行计数，区分或表征。 有利的是，系统可用于未扩增的DNA测序。

公开(公告)号：US07731826B2

公开(公告)日：2010-06-08

申请号：US11839793

申请日：2007-08-16

Applicant: Andrew D. Hibbs ,  Eric A. Duff

Inventor： Andrew D. Hibbs ,  Eric A. Duff

IPC: G01N27/26 ,  G01N27/28

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  C12Q2523/303 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: An electrolytic sensing system for measuring a blocking signal allows for controlled translocation of a molecule, such as DNA, through a fluid channel. A substantially constant electric field supplied by a DC source is applied across the fluid channel and induces translocation of the molecule within the system. An oscillating electric parameter (e.g. current or voltage) supplied by an AC source is also applied across the fluid channel as a means for measuring a blocking signal. The substantially constant electric field can be altered to provide more detailed control of the molecule and, optionally, run a select portion of the molecule through the channel multiple times to provide numerous signal readings. A temperature control stage cools the system, providing further control of molecule translocation. A modified or non-modified protein pore may be utilized in the fluid channel. The system allows for long DNA strands to be sequenced quickly without amplification.

Abstract translation: 用于测量阻塞信号的电解感测系统允许分子（例如DNA）通过流体通道的受控易位。 由DC源提供的基本上恒定的电场跨越流体通道施加并且诱导分子在系统内的易位。 由AC源提供的振荡电参数（例如电流或电压）也作为测量阻塞信号的装置跨越流体通道施加。 可以改变基本恒定的电场以提供对分子的更详细的控制，并且可选地，通过该通道多次运行分子的选择部分以提供大量的信号读数。 温度控制阶段冷却系统，进一步控制分子易位。 在流体通道中可以使用经修饰或未修饰的蛋白质孔。 该系统允许长DNA序列快速测序而无扩增。

公开(公告)号：US20090194429A1

公开(公告)日：2009-08-06

申请号：US12304543

申请日：2007-05-10

Applicant: Andrew D. Hibbs ,  Melissa Ann Poquette

Inventor： Andrew D. Hibbs ,  Melissa Ann Poquette

IPC: C12Q1/02 ,  G01N27/02

CPC classification number: G01N33/48728 ,  G01N33/48721

Abstract: An apparatus and method for sensing time varying ionic current in an electrolytic system having a first fluid chamber and a second fluid chamber separated by a barrier structure is provided, wherein the barrier structure includes thick walls and a substrate having an orifice therein, with the first and second fluid chambers being in communication via the orifice. A potential is applied between electrodes in respective first and second fluid chambers, thus driving an electrical current between them and through the orifice. Total capacitance of the system is less than 10 pF. Analytes are added to one of the first and second fluid chambers and time varying ionic current that passes across the orifice is measured. An amplifier proximal to the barrier structure and electrodes amplifies the ionic current signal.

Abstract translation: 提供一种用于感测电解系统中的时变离子电流的装置和方法，所述电解系统具有由阻挡结构隔开的第一流体室和第二流体室，其中所述阻挡结构包括厚壁和在其中具有孔口的基板， 并且第二流体室经由孔连通。 在相应的第一和第二流体室中的电极之间施加电位，从而驱动它们之间的电流并通过孔。 系统的总电容小于10 pF。 将分析物加入第一和第二流体室中的一个，并测量穿过孔口的时变离子电流。 靠近势垒结构的放大器和电极放大离子电流信号。

公开(公告)号：US07391210B2

公开(公告)日：2008-06-24

申请号：US11219664

申请日：2005-09-07

Applicant: Yongming Zhang ,  Andrew D. Hibbs ,  Robert Earl Grimm

Inventor： Yongming Zhang ,  Andrew D. Hibbs ,  Robert Earl Grimm

IPC: G01R33/02 ,  G01R33/04

CPC classification number: G01R33/04

Abstract: An integrated fluxgate-induction sensor is formed of a combined fluxgate sensor and induction sensor using a common core. The sensor may be in serial operation where it switches between a fluxgate mode for measuring static magnetic fields and an induction mode for measuring alternating magnetic fields. Additionally, the sensor may be used in an interleaved operation where the sensor operates from the fluxgate mode during the transition period where its core is changing from a high permeability state to a low permeability state or vice versa, while the sensor operates in the induction mode when the core is in its high permeability state. The resulting sensor provides for a compact magnetic sensor system capable of sensing magnetic fields which oscillate from zero frequency to 10 kHz and higher.

Abstract translation: 集成磁通门感应传感器由组合磁通门传感器和使用公共磁芯的感应传感器组成。 传感器可以在其中在用于测量静态磁场的磁通门模式和用于测量交变磁场的感应模式之间切换的串行操作中。 此外，传感器可以用于交错操作，其中传感器在其磁芯从高磁导率状态改变为低磁导率状态或反之亦然的过渡期期间从磁通门模式操作，而传感器在感应模式下工作 当芯处于高渗透性状态时。 所得到的传感器提供了一种能够感测从零频率振荡到10 kHz及更高​​频率的磁场的紧凑型磁传感器系统。

公开(公告)号：US07141968B2

公开(公告)日：2006-11-28

申请号：US10959480

申请日：2004-10-07

Applicant: Andrew D. Hibbs ,  Robert Matthews ,  David Matthew Jabson

Inventor： Andrew D. Hibbs ,  Robert Matthews ,  David Matthew Jabson

IPC: G01R33/02 ,  G01R27/26

CPC classification number: A61B5/04 ,  G01R29/14 ,  G01R33/02 ,  G01R35/005 ,  G01V3/08

Abstract: A compact sensor system integrates electric and/or magnetic field sensors to accurately measure, with a high level of sensitivity, one or more electric and magnetic vector components of fields. The electric and magnetic field data can be utilized separately or combined. The sensor system is self-contained so as to include a built-in power source, as well as data storage and/or transmission capability. The integrated sensor system also preferably includes a global positioning system (GPS) to provide timing and position information, a sensor unit which can determine the orientation and tilt of the sensor system, and self-calibrating structure which produces local electric and/or magnetic fields used to calibrate the sensor system following deployment.

Abstract translation: 紧凑的传感器系统集成电和/或磁场传感器，以高灵敏度精确地测量一个或多个电场和磁场矢量分量。 电场和磁场数据可以单独使用或组合使用。 传感器系统是独立的，以便包括内置电源，以及数据存储和/或传输能力。 集成传感器系统还优选地包括提供定时和位置信息的全球定位系统（GPS），可以确定传感器系统的取向和倾斜的传感器单元以及产生局部电场和/或磁场的自校准结构 用于在部署后校准传感器系统。

公开(公告)号：US5574369A

公开(公告)日：1996-11-12

申请号：US293094

申请日：1994-08-19

Applicant: Andrew D. Hibbs

Inventor： Andrew D. Hibbs

IPC: G01R33/035 ,  H03D9/00 ,  H03K17/92

CPC classification number: H03D9/00 ,  G01R33/0354 ,  Y10S505/846 ,  Y10S505/861

Abstract: A sensitive detector and detector system with multi-state element or elements employing the phenomenon of stochastic resonance to enhance signal-to-noise ratio (SNR) and detector sensitivity. Signal output is enhanced by the addition of external noise at the input. A single detector element has these improved outputs. Several stochastic resonance elements may be connected in arrays to further increase SNR of the output, increase detector sensitivity, and linearize the relation between input and output.

Abstract translation: 具有采用随机共振现象的多态元件或元件的灵敏检测器和检测器系统，以增强信噪比（SNR）和检测器灵敏度。 通过在输入端添加外部噪声来增强信号输出。 单个检测器元件具有这些改进的输出。 可以将阵列中的几个随机谐振元件连接起来以进一步增加输出的SNR，提高检测器灵敏度，并将输入和输出之间的关系线性化。

公开(公告)号：US09201058B2

公开(公告)日：2015-12-01

申请号：US12304543

申请日：2007-05-10

Applicant: Andrew D. Hibbs ,  Melissa Ann Poquette

Inventor： Andrew D. Hibbs ,  Melissa Ann Poquette

IPC: G01N33/487

CPC classification number: G01N33/48728 ,  G01N33/48721

Abstract: An apparatus and method for sensing time varying ionic current in an electrolytic system having a first fluid chamber and a second fluid chamber separated by a barrier structure is provided, wherein the barrier structure includes thick walls and a substrate having an orifice therein, with the first and second fluid chambers being in communication via the orifice. A potential is applied between electrodes in respective first and second fluid chambers, thus driving an electrical current between them and through the orifice. Total capacitance of the system is less than 10 pF. Analytes are added to one of the first and second fluid chambers and time varying ionic current that passes across the orifice is measured. An amplifier proximal to the barrier structure and electrodes amplifies the ionic current signal.

Abstract translation: 提供一种用于感测电解系统中的时变离子电流的装置和方法，所述电解系统具有由阻挡结构隔开的第一流体室和第二流体室，其中所述阻挡结构包括厚壁和在其中具有孔口的基板， 并且第二流体室经由孔连通。 在相应的第一和第二流体室中的电极之间施加电位，从而驱动它们之间的电流并通过孔。 系统的总电容小于10 pF。 将分析物加入第一和第二流体室中的一个，并测量穿过孔口的时变离子电流。 靠近势垒结构的放大器和电极放大离子电流信号。

公开(公告)号：US08283157B2

公开(公告)日：2012-10-09

申请号：US12999157

申请日：2009-06-18

Applicant: Andrew D Hibbs ,  Geoffrey Alden Barrall ,  Eric N Ervin ,  Daniel K Lathrop

Inventor： Andrew D Hibbs ,  Geoffrey Alden Barrall ,  Eric N Ervin ,  Daniel K Lathrop

IPC: C12M1/00

CPC classification number: G01N33/48721

Abstract: An electrolytic system includes an analyte chamber having an access port for introducing a sample containing a molecules of interest, such as DNA. Electrodes create an electric field along a length of the analyte chamber to drive molecules toward an interaction region containing a nanopore, thereby increasing the arrival rate of molecules at the nanopore. Additional electrodes may be utilized to create an electric field through the nanopore to drive a molecule into the nanopore. A current sensor may be utilized to count, discriminate or characterize the molecules as they interact with the nanopore. Advantageously, system can be utilized for unamplified DNA sequencing.

Abstract translation: 电解系统包括分析物室，其具有用于引入含有目标分子（例如DNA）的样品的入口。 电极沿分析物室的长度产生电场，以将分子驱向包含纳米孔的相互作用区域，从而增加分子在纳米孔处的到达速率。 可以使用附加的电极来产生通过纳米孔的电场以将分子驱动到纳米孔中。 当电流传感器与纳米孔相互作用时，可以使用电流传感器对分子进行计数，区分或表征。 有利的是，系统可用于未扩增的DNA测序。

公开(公告)号：US20080041733A1

公开(公告)日：2008-02-21

申请号：US11839793

申请日：2007-08-16

Applicant: Andrew D. Hibbs ,  Eric A. Duff

Inventor： Andrew D. Hibbs ,  Eric A. Duff

IPC: G01N27/26 ,  G01N27/28

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  C12Q2523/303 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: An electrolytic sensing system for measuring a blocking signal allows for controlled translocation of a molecule, such as DNA, through a fluid channel. A substantially constant electric field supplied by a DC source is applied across the fluid channel and induces translocation of the molecule within the system. An oscillating electric parameter (e.g. current or voltage) supplied by an AC source is also applied across the fluid channel as a means for measuring a blocking signal. The substantially constant electric field can be altered to provide more detailed control of the molecule and, optionally, run a select portion of the molecule through the channel multiple times to provide numerous signal readings. A temperature control stage cools the system, providing further control of molecule translocation. A modified or non-modified protein pore may be utilized in the fluid channel. The system allows for long DNA strands to be sequenced quickly without amplification.

Abstract translation: 用于测量阻塞信号的电解感测系统允许分子（例如DNA）通过流体通道的受控易位。 由DC源提供的基本上恒定的电场跨越流体通道施加并且诱导分子在系统内的易位。 由AC源提供的振荡电参数（例如电流或电压）也作为测量阻塞信号的装置跨越流体通道施加。 可以改变基本恒定的电场以提供对分子的更详细的控制，并且可选地，通过该通道多次运行分子的选择部分以提供大量的信号读数。 温度控制阶段冷却系统，进一步控制分子易位。 在流体通道中可以使用经修饰或未修饰的蛋白质孔。 该系统允许长DNA序列快速测序而无扩增。

公开(公告)号：US06961601B2

公开(公告)日：2005-11-01

申请号：US10459267

申请日：2003-06-11

Applicant: Robert Matthews ,  Michael A. Krupka ,  Andrew D. Hibbs

Inventor： Robert Matthews ,  Michael A. Krupka ,  Andrew D. Hibbs

IPC: A61B5/04

CPC classification number: A61B5/0408 ,  A61B5/0478 ,  A61B5/6805 ,  G01R15/165

Abstract: A system for measuring a biopotential signal produced by a body in free space at a location adjacent to the body, and if desired, through clothing, includes a probe that can be positioned adjacent to the body. The probe includes a conductive electrode to receive the biopotential signal and a conductor that is maintained at a fixed distance from the electrode. The potential of the conductor can be maintained substantially equal to the potential of the electrode to shield the electrode from stray electrical noise. The system further includes a high impedance first stage amplifier that is incorporated into the probe and electrically connected to the electrode using a relatively short connector to minimize connector noise. Functionally, the first stage amplifier compares the electrical potential of the electrode to a second potential (e.g. a local ground) and generates a signal that is indicative of the biopotential.

Abstract translation: 用于测量由身体在与身体相邻的位置处的自由空间中产生的生物电位信号的系统，并且如果需要，通过衣物测量，包括可以邻近身体定位的探针。 探针包括用于接收生物电位信号的导电电极和与电极保持固定距离的导体。 导体的电位可以保持基本上等于电极屏蔽电极免受杂散电噪声的电位。 该系统还包括一个高阻抗的第一级放大器，该放大器被并入到探针中并且使用相对短的连接器电连接到电极以最小化连接器噪声。 功能上，第一级放大器将电极的电位与第二电位（例如局部地）进行比较，并产生指示生物电位的信号。