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26 条记录 (耗时 0.019 秒)

    Method and device for forming a three-dimensional arrangement of biological cells
    11.
    发明授权
    Method and device for forming a three-dimensional arrangement of biological cells 有权
    用于形成生物细胞三维排列的方法和装置

    公开(公告)号：US08546117B2

    公开(公告)日：2013-10-01

    申请号：US12664933

    申请日：2008-06-06

    申请人： Charli Kruse ,  Philipp Ciba ,  Guenter Fuhr

    发明人： Charli Kruse ,  Philipp Ciba ,  Guenter Fuhr

    IPC分类号： C12N11/00 ,  C12N11/16 ,  C12P1/00

    CPC分类号： C12N5/0068 ,  C12N5/0062 ,  C12N2533/30

    摘要： A method for forming a three-dimensional cell arrangement (1) of biological cells is disclosed, including the steps of preparation of the cell arrangement (1) on a flexible substrate (10) and deformation of the substrate (10), wherein the deformation of the substrate (10) is brought about by an attractive force exerted by the cells (2) on the substrate (10). A substrate (10), is also disclosed, made from a flexible material and including a substrate surface (11) for adhesion of a cell arrangement (1) of biological cells, wherein the substrate surface (11) has a number of force attachment points arranged to exert an attraction force which may be transmitted from the cells to the substrate (10) and the substrate (10) has a flexibility such that the substrate (10) is deformable with the action of the attraction force.

    摘要翻译： 公开了一种用于形成生物细胞的三维细胞装置（1）的方法，包括在柔性基底（10）上制备细胞装置（1）和基底（10）变形的步骤，其中变形 通过由电池（2）施加在衬底（10）上的吸引力引起衬底（10）的移动。 还公开了由柔性材料制成并且包括用于粘附生物细胞的细胞装置（1）的基底表面（11）的基底（10），其中所述基底表面（11）具有多个力附着点 布置成施加可以从单元传递到基板（10）的吸引力，并且基板（10）具有柔性，使得基板（10）可以在吸引力的作用下变形。

    METHOD AND DEVICE FOR FORMING A THREE-DIMENSIONAL ARRANGEMENT OF BIOLOGICAL CELLS
    12.
    发明申请
    METHOD AND DEVICE FOR FORMING A THREE-DIMENSIONAL ARRANGEMENT OF BIOLOGICAL CELLS 有权
    用于形成生物细胞三维布置的方法和装置

    公开(公告)号：US20100190249A1

    公开(公告)日：2010-07-29

    申请号：US12664933

    申请日：2008-06-06

    申请人： Charli Kruse ,  Philipp Ciba ,  Guenter Fuhr

    发明人： Charli Kruse ,  Philipp Ciba ,  Guenter Fuhr

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0068 ,  C12N5/0062 ,  C12N2533/30

    摘要： A method for forming a three-dimensional cell arrangement (1) of biological cells is disclosed, including the steps of preparation of the cell arrangement (1) on a flexible substrate (10) and deformation of the substrate (10), wherein the deformation of the substrate (10) is brought about by an attractive force exerted by the cells (2) on the substrate (10). A substrate (10), is also disclosed, made from a flexible material and including a substrate surface (11) for adhesion of a cell arrangement (1) of biological cells, wherein the substrate surface (11) has a number of force attachment points arranged to exert an attraction force which may be transmitted from the cells to the substrate (10) and the substrate (10) has a flexibility such that the substrate (10) is deformable with the action of the attraction force.

    摘要翻译： 公开了一种用于形成生物细胞的三维细胞装置（1）的方法，包括在柔性基底（10）上制备细胞装置（1）和基底（10）变形的步骤，其中变形 通过由电池（2）施加在衬底（10）上的吸引力引起衬底（10）的移动。 还公开了由柔性材料制成并且包括用于粘附生物细胞的细胞装置（1）的基底表面（11）的基底（10），其中所述基底表面（11）具有多个力附着点 布置成施加可以从单元传递到基板（10）的吸引力，并且基板（10）具有柔性，使得基板（10）可以在吸引力的作用下变形。

    Method for the Production of a Cell Composition Containing Epithelial Cells
    13.
    发明申请
    Method for the Production of a Cell Composition Containing Epithelial Cells 有权
    含上皮细胞的细胞组合物的制备方法

    公开(公告)号：US20080193420A1

    公开(公告)日：2008-08-14

    申请号：US11547678

    申请日：2005-04-01

    申请人： Charli Kruse ,  Gunter Fuhr ,  Thilo Wedel

    发明人： Charli Kruse ,  Gunter Fuhr ,  Thilo Wedel

    IPC分类号： A61K48/00 ,  C12N5/02 ,  C12M1/00

    CPC分类号： C12N5/0677 ,  A61K35/24 ,  A61K35/26

    摘要： Disclosed is a method for forming epithelial cells. Said method comprises the steps of aggregating stem cells from differentiated exocrine gland tissue to obtain an organoid body and differentiating at least one portion of the organoid body or a tissue body grown therefrom to obtain epithelial cells. Also disclosed is a cultivation device, particularly for forming differential epithelial cells.

    摘要翻译： 公开了形成上皮细胞的方法。 所述方法包括以下步骤：从分化的外分泌腺组织聚集干细胞以获得有机体，并分化至少一部分有机体或由其生长的组织体以获得上皮细胞。 还公开了一种培养装置，特别是用于形成差异上皮细胞的培养装置。

    Spontaneously contracting fish cell aggregates, use thereof and method for the production thereof
    14.
    发明授权
    Spontaneously contracting fish cell aggregates, use thereof and method for the production thereof 有权
    自发收缩鱼细胞聚集体，其用途及其生产方法

    公开(公告)号：US08735151B2

    公开(公告)日：2014-05-27

    申请号：US13394586

    申请日：2010-09-08

    申请人： Charli Kruse ,  Bianka Grunow ,  Daniel Hans Rapoport ,  Philipp Ciba

    发明人： Charli Kruse ,  Bianka Grunow ,  Daniel Hans Rapoport ,  Philipp Ciba

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0657 ,  A61K35/00 ,  C12N2509/00

    摘要： The invention relates to an in vitro method for producing spontaneously contracting fish cell aggregates, to the fish cell aggregates obtained thereby, and to the use thereof, in particular for testing biological active substances and pharmaceuticals. The in vitro method according to the invention for producing contracting fish cell aggregates comprises the following steps: a) mechanical comminution and/or partial enzymatic digestion of fish embryos or fish larvae; b) transfer of the comminuted and/or partially digested fish tissue into an enzyme-free medium and removing the supernatant by centrifuging to obtain a cell pellet; c) re-suspension of the cell pellet in a cell culture medium; d) culturing the cells, with the medium being changed at least once, until spontaneously contracting cell aggregates form.

    摘要翻译： 本发明涉及用于生产自发收缩的鱼细胞聚集体的体外方法，以及由此获得的鱼细胞聚集体及其用途，特别是用于测试生物活性物质和药物的用途。 根据本发明的用于产生收缩性鱼细胞聚集体的体外方法包括以下步骤：a）鱼胚胎或鱼幼体的机械粉碎和/或部分酶消化; b）将经粉碎和/或部分消化的鱼组织转移到无酶培养基中并通过离心除去上清液以获得细胞沉淀; c）细胞沉淀在细胞培养基中的再悬浮; d）培养细胞，将培养基改变至少一次，直到形成自发收缩的细胞聚集体。

    Method and device for forming biologic cell aggregates
    15.
    发明授权
    Method and device for forming biologic cell aggregates 有权
    用于形成生物细胞聚集体的方法和装置

    公开(公告)号：US08304237B2

    公开(公告)日：2012-11-06

    申请号：US12665325

    申请日：2008-06-13

    申请人： Charli Kruse ,  Sandra Danner

    发明人： Charli Kruse ,  Sandra Danner

    IPC分类号： C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/0062 ,  C12M21/08 ,  C12M25/14

    摘要： The invention relates to a method for cultivating biologic cells (1), wherein cells (1) are grown on a substrate (10) having a plurality of substrate openings (11), and wherein cell aggregates (2) are formed, including groups of cells (1) that span the substrate openings (11). A separation of the cell aggregates (2) from the substrate (10) by extracting the cell aggregates (2) from the substrate openings (11) can be provided. The invention further relates to a cell-cultivating device (100), including a substrate (10) having a plurality of substrate openings (11) and cell aggregates (2) including groups of cells (1) that span the substrate openings (11). The cell aggregates (2) are particularly used in high throughput tests with biologically active substances or in methods of tissue cultivation.

    摘要翻译： 本发明涉及一种培养生物细胞的方法（1），其中细胞（1）在具有多个基底开口（11）的基底（10）上生长，并且其中形成细胞聚集体（2），包括 跨越衬底开口（11）的电池（1）。 可以提供通过从基板开口（11）提取细胞聚集体（2）而将细胞聚集体（2）与基底（10）分离。 本发明还涉及一种细胞培养装置（100），其包括具有多个基板开口（11）的基板（10）和包括跨越基板开口（11）的电池组（1）的电池集合体（2） 。 细胞聚集体（2）特别用于具有生物活性物质或组织培养方法的高通量测试中。

    SPONTANEOUSLY CONTRACTING FISH CELL AGGREGATES, USE THEREOF AND METHOD FOR THE PRODUCTION THEREOF
    17.
    发明申请
    SPONTANEOUSLY CONTRACTING FISH CELL AGGREGATES, USE THEREOF AND METHOD FOR THE PRODUCTION THEREOF 有权
    自发收缩鱼细胞聚集体及其生产方法

    公开(公告)号：US20120171695A1

    公开(公告)日：2012-07-05

    申请号：US13394586

    申请日：2010-09-08

    申请人： Charli Kruse ,  Bianka Grunow ,  Daniel Hans Rapoport ,  Philipp Ciba

    发明人： Charli Kruse ,  Bianka Grunow ,  Daniel Hans Rapoport ,  Philipp Ciba

    IPC分类号： C12Q1/02 ,  G01N33/53 ,  C12N5/071

    CPC分类号： C12N5/0657 ,  A61K35/00 ,  C12N2509/00

    摘要： The invention relates to an in vitro method for producing spontaneously contracting fish cell aggregates, to the fish cell aggregates obtained thereby, and to the use thereof, in particular for testing biological active substances and pharmaceuticals. The in vitro method according to the invention for producing contracting fish cell aggregates comprises the following steps: a) mechanical comminution and/or partial enzymatic digestion of fish embryos or fish larvae; b) transfer of the comminuted and/or partially digested fish tissue into an enzyme-free medium and removing the supernatant by centrifuging to obtain a cell pellet; c) re-suspension of the cell pellet in a cell culture medium; d) culturing the cells, with the medium being changed at least once, until spontaneously contracting cell aggregates form.

    摘要翻译： 本发明涉及用于生产自发收缩的鱼细胞聚集体的体外方法，以及由此获得的鱼细胞聚集体及其用途，特别是用于测试生物活性物质和药物的用途。 根据本发明的用于产生收缩性鱼细胞聚集体的体外方法包括以下步骤：a）鱼胚胎或鱼幼体的机械粉碎和/或部分酶消化; b）将经粉碎和/或部分消化的鱼组织转移到无酶培养基中并通过离心除去上清液以获得细胞沉淀; c）细胞沉淀在细胞培养基中的再悬浮; d）培养细胞，将培养基改变至少一次，直到形成自发收缩的细胞聚集体。

    METHOD AND APPARATUS FOR CONSERVING DEEP-SEA ORGANISMS
    18.
    发明申请
    METHOD AND APPARATUS FOR CONSERVING DEEP-SEA ORGANISMS 有权
    用于保护深海有机体的方法和装置

    公开(公告)号：US20120079990A1

    公开(公告)日：2012-04-05

    申请号：US13319182

    申请日：2010-06-14

    申请人： Guenter R. Fuhr ,  Heiko Zimmermann ,  Charli Kruse ,  Robert Lemor

    发明人： Guenter R. Fuhr ,  Heiko Zimmermann ,  Charli Kruse ,  Robert Lemor

    IPC分类号： A01N1/02 ,  A01M23/00 ,  A01K61/00

    CPC分类号： A01K63/02 ,  A01N1/00 ,  A01N1/0221 ,  A01N1/0252 ,  A01N1/0284

    摘要： The invention relates to a method for conserving at least one biological deep-sea organism (1) comprising the steps of collecting the biological organism (1) in a deep-sea trapping device (110); cooling the biological organism (1) with a cooling device (120), wherein the biological organism (1) is put in a frozen condition; and moving the frozen biological organism (1), preferably with the trapping device (110), to a normal-pressure environment. The invention further relates to a conservation apparatus (100) for conserving at least one biological deep-sea organism (1).

    摘要翻译： 本发明涉及一种用于保存至少一种生物深海生物体的方法（1），包括以下步骤：在深海捕获装置（110）中收集生物体（1）; 用冷却装置（120）冷却生物体（1），其中所述生物体（1）处于冷冻状态; 以及将所述冷冻生物体（1）优选地与所述捕集装置（110）一起移动到常压环境。 本发明还涉及一种用于保存至少一种生物深海生物（1）的保存装置（100）。