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    Methods and compositions for assaying homocysteine
    11.
    发明授权
    Methods and compositions for assaying homocysteine 有权
    用于测定同型半胱氨酸的方法和组合物

    公开(公告)号：US08476034B2

    公开(公告)日：2013-07-02

    申请号：US11969803

    申请日：2008-01-04

    申请人： Chong-Sheng Yuan ,  Abhijit Datta ,  Chao Dou

    发明人： Chong-Sheng Yuan ,  Abhijit Datta ,  Chao Dou

    IPC分类号： C12Q1/48

    CPC分类号： C12Q1/34 ,  C12Q1/48 ,  G01N33/6815 ,  G01N2333/91011

    摘要： This invention relates generally to the field of homocysteine detection. In particular, the invention provides a method for determining homocysteine presence or concentration in samples, which method comprises: contacting a sample containing or suspected of containing Hcy with a Hcy co-substrate and a Hcy converting enzyme in a Hcy conversion reaction to form a Hcy conversion product and a Hcy co-substrate conversion product; and assessing the Hcy co-substrate conversion product to determine the presence, absence and/or amount of the Hcy in the sample. The Hcy co-substrate conversion product may be assessed directly, or it may be assessed by further conversion of the Hcy co-substrate conversion product into another material by the action of one or more additional enzymes. A kit for assaying homocysteine based on the same principle is also provided.

    摘要翻译： 本发明一般涉及同型半胱氨酸检测领域。 特别地，本发明提供了一种测定样品中同型半胱氨酸存在或浓度的方法，该方法包括：在Hcy转化反应中使包含或怀疑含有Hcy的样品与Hcy共底物和Hcy转化酶接触以形成Hcy 转化产物和Hcy共底物转化产物; 并评估Hcy共底物转化产物以确定样品中Hcy的存在，不存在和/或量。 可以直接评估Hcy共底物转化产物，或者可以通过一种或多种另外的酶的作用将Hcy共底物转化产物进一步转化成另一种物质来评估。 还提供了用于基于相同原理测定同型半胱氨酸的试剂盒。

    Methods for assaying percentage of glycated hemoglobin
    12.
    发明授权
    Methods for assaying percentage of glycated hemoglobin 有权
    测定糖化血红蛋白百分比的方法

    公开(公告)号：US08318501B2

    公开(公告)日：2012-11-27

    申请号：US12944586

    申请日：2010-11-11

    申请人： Chong-Sheng Yuan ,  Abhijit Datta ,  Limin Liu

    发明人： Chong-Sheng Yuan ,  Abhijit Datta ,  Limin Liu

    IPC分类号： G01N33/72 ,  G01N33/00

    CPC分类号： G01N33/723 ,  Y10T436/104998 ,  Y10T436/105831 ,  Y10T436/107497 ,  Y10T436/25375

    摘要： The invention provides enzymatic methods for direct determination of percentage of glycated hemoglobin in blood samples without the need of a separated measurement of total hemoglobin content in blood samples. The methods utilizes one or two different types of oxidizing agents which selectively oxidize low-molecular weight reducing substances and high-molecular weight (mainly hemoglobin) reducing substances in blood samples, coupled with enzymatic reactions catalyzed by proteases, fructosyl amino acid oxidase, and peroxidase. The invention provides kits for performing the methods of the invention.

    摘要翻译： 本发明提供用于直接测定血液样品中糖化血红蛋白百分比的酶法，而不需要分离测量血液样品中的总血红蛋白含量。 该方法使用一种或两种不同类型的氧化剂，其选择性地氧化血液样品中的低分子量还原物质和高分子量（主要是血红蛋白）还原物质，加上由蛋白酶，果糖基氨基酸氧化酶和过氧化物酶催化的酶反应 。 本发明提供了用于实施本发明方法的试剂盒。

    Methods for assaying percentage of glycated hemoglobin
    14.
    发明申请
    Methods for assaying percentage of glycated hemoglobin 有权
    测定糖化血红蛋白百分比的方法

    公开(公告)号：US20080096230A1

    公开(公告)日：2008-04-24

    申请号：US11881179

    申请日：2007-07-25

    申请人： Chong-Sheng Yuan ,  Abhijit Datta ,  Limin Liu

    发明人： Chong-Sheng Yuan ,  Abhijit Datta ,  Limin Liu

    IPC分类号： G01N33/53

    CPC分类号： G01N33/723 ,  Y10T436/104998 ,  Y10T436/105831 ,  Y10T436/107497 ,  Y10T436/25375

    摘要： The invention provides enzymatic methods for direct determination of percentage of glycated hemoglobin in blood samples without the need of a separated measurement of total hemoglobin content in blood samples. The methods utilizes one or two different types of oxidizing agents which selectively oxidize low-molecular weight reducing substances and high-molecular weight (mainly hemoglobin) reducing substances in blood samples, coupled with enzymatic reactions catalyzed by proteases, fructosyl amino acid oxidase, and peroxidase. The invention provides kits for performing the methods of the invention.

    摘要翻译： 本发明提供用于直接测定血液样品中糖化血红蛋白百分比的酶法，而不需要分离测量血液样品中的总血红蛋白含量。 该方法使用一种或两种不同类型的氧化剂，其选择性地氧化血液样品中的低分子量还原物质和高分子量（主要是血红蛋白）还原物质，加上由蛋白酶，果糖基氨基酸氧化酶和过氧化物酶催化的酶反应 。 本发明提供了用于实施本发明方法的试剂盒。

    Reversible inhibitors of S-adenosyl-L-homocysteine hydrolase and uses thereof
    15.
    发明申请
    Reversible inhibitors of S-adenosyl-L-homocysteine hydrolase and uses thereof 有权
    S-腺苷-L-高半胱氨酸水解酶的可逆抑制剂及其用途

    公开(公告)号：US20070129386A1

    公开(公告)日：2007-06-07

    申请号：US11649996

    申请日：2007-01-05

    申请人： Chong-Sheng Yuan

    发明人： Chong-Sheng Yuan

    IPC分类号： A61K31/522 ,  A61K31/52 ,  C07D473/12 ,  C07D473/10

    CPC分类号： C07D473/34 ,  A61K31/52 ,  A61K45/06 ,  G06F19/16 ,  G06F19/18 ,  A61K2300/00

    摘要： The present invention provides compositions and methods for reversibly inhibiting S-adenosyl-L-homocysteine (SAH) hydrolase. The compounds of the present invention can be used in combination with an anti-hemorrhagic viral infection agent, an immunosuppressant, a homocysteine lowering agent, or an anti-neoplasm agent. The compositions and methods of the present invention can be used for the prevention and treatment of hemorrhagic virus infection, autoimmune diseases, autograft rejection, neoplasm, hyperhomocysteineuria, cardiovascular disease, stroke, Alzheimer's disease, or diabetes.

    摘要翻译： 本发明提供了可逆抑制S-腺苷-L-高半胱氨酸（SAH）水解酶的组合物和方法。 本发明的化合物可以与抗出血性病毒感染剂，免疫抑制剂，同型半胱氨酸降低剂或抗肿瘤剂组合使用。 本发明的组合物和方法可用于预防和治疗出血性病毒感染，自身免疫疾病，自体移植排斥反应，肿瘤，高半胱氨酸尿症，心血管疾病，中风，阿尔茨海默病或糖尿病。

    Allosteric enzyme coupled immunoassay (AECIA)
    17.
    发明申请
    Allosteric enzyme coupled immunoassay (AECIA) 审中-公开
    变构酶联合免疫测定（AECIA）

    公开(公告)号：US20060166236A1

    公开(公告)日：2006-07-27

    申请号：US11304010

    申请日：2005-12-15

    申请人： Chong-Sheng Yuan

    发明人： Chong-Sheng Yuan

    IPC分类号： C12Q1/68 ,  G01N33/566

    CPC分类号： G01N33/78 ,  C12Q1/485 ,  G01N33/542 ,  G01N33/54393 ,  G01N33/581 ,  G01N2333/91215

    摘要： The present invention is directed to methods and compositions for an allosteric enzyme coupled assay, and preferably to an allosteric enzyme coupled immunoassay (AECIA). The assay uses an allosteric enzyme to generate a readout signal. The assay is based on the competition between an analyte in a sample and an analyte or analyte analog conjugated to an allosteric regulator with a specific binding reagent for the analyte. In the absence of any analyte in the sample, an analyte or an analyte analog conjugated to an allosteric regulator binds to the specific binding reagent and such binding prevents or reduces the allosteric regulator's regulation, e.g., activation, on the allosteric enzyme. An analyte, if present in the sample, competes with the analyte or analyte analog conjugated to the allosteric regulator for binding with the specific binding reagent, reduces or prevents binding of the specific binding reagent to the analyte or analyte analog conjugated to the allosteric regulator, leading to increased regulation, e.g., activation, of the enzyme. 1-substituted-β-D-fructofuranose 2,6-bisphosphate compounds and conjugates comprising the same are provided. Kits comprising the conjugates, and methods using the conjugates for assaying an analyte are further provided.

    摘要翻译： 本发明涉及用于变构酶偶联测定法，优选变构酶偶联免疫测定法（AECIA）的方法和组合物。 该测定使用变构酶来产生读出信号。 该测定法基于样品中的分析物与与变构调节剂缀合的分析物或分析物类似物与分析物的特异性结合试剂之间的竞争。 在样品中没有任何分析物的情况下，与变构调节物缀合的分析物或分析物类似物与特异性结合试剂结合，并且这种结合在变构酶上阻止或减少变构调节物的调节，例如活化。 分析物（如果存在于样品中）与与变构调节剂缀合的分析物或分析物类似物与特异性结合试剂结合竞争，减少或防止特异性结合试剂与分子或与变构调节物结合的分析物类似物的结合， 导致酶的调节，例如活化。 提供了1-取代-β-D-呋喃果糖2,6-二磷酸酯化合物和包含它们的共轭物。 还提供了包含缀合物的试剂盒和使用共轭物分析分析物的方法。

    Reversible inhibitors of S-adenosyl-L-homocysteine hydrolase and uses thereof
    19.
    发明申请
    Reversible inhibitors of S-adenosyl-L-homocysteine hydrolase and uses thereof 有权
    S-腺苷-L-高半胱氨酸水解酶的可逆抑制剂及其用途

    公开(公告)号：US20050182075A1

    公开(公告)日：2005-08-18

    申请号：US10964236

    申请日：2004-10-13

    申请人： Chong-Sheng Yuan

    发明人： Chong-Sheng Yuan

    IPC分类号： A61K31/52 ,  A61K45/06 ,  C07D473/34 ,  G06F19/00 ,  A61K31/4745 ,  C07D471/02 ,  C07D473/14

    CPC分类号： C07D471/04 ,  A61K31/52 ,  A61K45/06 ,  C07D473/34 ,  G06F19/16 ,  G06F19/18 ,  A61K2300/00

    摘要： The present invention provides compositions and methods for reversibly inhibiting S-adenosyl-L-homocysteine (SAH) hydrolase. The compounds of the present invention can be used in combination with an anti-hemorrhagic viral infection agent, an immunosuppressant, a homocysteine lowering agent, or an anti-neoplasm agent. The compositions and methods of the present invention can be used for the prevention and treatment of hemorrhagic virus infection, autoimmune diseases, autograft rejection, neoplasm, hyperhomocysteineuria, cardiovascular disease, stroke, Alzheimer's disease, or diabetes.

    摘要翻译： 本发明提供了可逆抑制S-腺苷-L-高半胱氨酸（SAH）水解酶的组合物和方法。 本发明的化合物可以与抗出血性病毒感染剂，免疫抑制剂，同型半胱氨酸降低剂或抗肿瘤剂组合使用。 本发明的组合物和方法可用于预防和治疗出血性病毒感染，自身免疫疾病，自体移植排斥反应，肿瘤，高半胱氨酸尿症，心血管疾病，中风，阿尔茨海默病或糖尿病。

    Use of reversible inhibitors of S-adenosyl-L-homocysteine hydrolase for treating lupus
    20.
    发明授权
    Use of reversible inhibitors of S-adenosyl-L-homocysteine hydrolase for treating lupus 有权
    使用S-腺苷-L-高半胱氨酸水解酶的可逆抑制剂治疗狼疮

    公开(公告)号：US07868011B2

    公开(公告)日：2011-01-11

    申请号：US11650089

    申请日：2007-01-05

    申请人： Chong-Sheng Yuan

    发明人： Chong-Sheng Yuan

    IPC分类号： A61P31/06 ,  A61K31/52 ,  C07D473/34 ,  A61P35/00 ,  A61P31/14 ,  C07D471/04

    CPC分类号： A61K31/437 ,  A61K31/52

    摘要： The present invention provides methods for suppressing autoimmunity in a patient in need of treatment for systemic lupus erythematosus by administering to the patient an effective amount of methyl 4-(Adenin-9-yl)-2-hydroxybutanoate or pharmaceutically acceptable salts thereof. In some embodiments, the claimed methods include co-administering an effective amount of an immunosuppressant.

    摘要翻译： 本发明提供了通过向患者施用有效量的4-（腺嘌呤-9-基）-2-羟基丁酸甲酯或其药学上可接受的盐来抑制需要治疗系统性红斑狼疮的患者中的自身免疫的方法。 在一些实施方案中，所要求保护的方法包括共同施用有效量的免疫抑制剂。