公开(公告)号：US20070065828A1

公开(公告)日：2007-03-22

申请号：US10597173

申请日：2005-01-14

申请人： Cheol-Min Kim ,  Hee-Kyung Park ,  Hyun-Jung Jang ,  Hyo-Myoung Kim

发明人： Cheol-Min Kim ,  Hee-Kyung Park ,  Hyun-Jung Jang ,  Hyo-Myoung Kim

IPC分类号： C12Q1/68 ,  C12M3/00

CPC分类号： C12Q1/689

摘要： The present invention relates to a method for detecting Mycoplasma and its related strains which are source of contamination of cell lines and biological products and human pathogenic. More particularly, the present invention relates to genus-specific and species-specific oligonucleotides for genotyping of Mycoplasma, Acholeplasm and Ureaplasma strains, microarray comprising the oligonucleotides, and method for detection of species using the microarray. As described above, the present invention provides a rapid and accurate assay method capable of simultaneously detecting many Mycoplasma and its related strains from a single sample using a microarray comprising novel oligonucleotides for detecting Mycoplasma and its related strains which are known as a source of contamination of cell lines and biological products and human pathogenic. Further, the present invention provides an objective and credible assay method capable of tracing a contamination source for preventing expansion of infective Mycoplasma and its related strains and controlling a contamination of Mycoplasma against biological products and stem cells or cord blood cells which are useful for gene therapy and cell therapy.

摘要翻译： 本发明涉及一种检测支原体及其相关菌株的方法，这些菌株是细胞系和生物制品的污染源和人类致病菌。 更具体地说，本发明涉及用于基因分型支原体，痘苗和解脲支原体菌株的特异性和物种特异性寡核苷酸，包含寡核苷酸的微阵列，以及使用微阵列检测物种的方法。 如上所述，本发明提供了一种快速且准确的测定方法，其能够使用包含用于检测支原体及其相关菌株的微阵列的微阵列同时检测许多支原体及其相关菌株，所述新型寡核苷酸及其相关菌株被称为污染源 细胞系和生物制品和人类致病性。 此外，本发明提供了一种客观且可靠的测定方法，其能够追踪污染源以防止感染性支原体及其相关菌株的扩增，并控制支原体对生物制品和干细胞或脐带血细胞的污染，这些基因治疗可用于基因治疗 和细胞治疗。

公开(公告)号：US20110111416A1

公开(公告)日：2011-05-12

申请号：US12991364

申请日：2009-03-27

申请人： Hee Kyung Park ,  Jae Jin Choi

发明人： Hee Kyung Park ,  Jae Jin Choi

IPC分类号： C12Q1/68 ,  C07K9/00

CPC分类号： C12Q1/6876 ,  C12Q1/6837 ,  C12Q2600/158 ,  C12Q2600/178 ,  C12Q2525/207 ,  C12Q2525/107

摘要： Disclosed are peptide nucleic acid (PNA) probes, a kit and a method for expression profiling of microRNAs (miRNAs), which play an important role in regulation of expression of genes encoding proteins.

摘要翻译： 公开了肽核酸（PNA）探针，试剂盒和微小RNA（miRNA）表达谱的方法，其在调节编码蛋白质的基因表达中起重要作用。

公开(公告)号：US20100292471A1

公开(公告)日：2010-11-18

申请号：US12299665

申请日：2007-12-17

申请人： Hee Kyung Park ,  Jong Chan Lim ,  Sung Jin Ha ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Jin Won Yun

发明人： Hee Kyung Park ,  Jong Chan Lim ,  Sung Jin Ha ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Jin Won Yun

IPC分类号： C07D473/18

CPC分类号： C07H21/00 ,  C07K14/003

摘要： Disclosed is a PNA oligomer with increased solubility in water and specificity upon hybridization with nucleic acid, which comprises at least one universal base, capable of forming a base pair with natural DNA or RNA bases, incorporated in its base sequence, the base sequence having at least 60% purine bases or at least four contiguous purine bases.

摘要翻译： 公开了一种具有增加的在水中的溶解度和与核酸杂交的特异性的PNA低聚物，该核酸包含至少一个通用碱基，其能够与天然的DNA或RNA碱基形成碱基对，并且其碱基序列具有 至少60％的嘌呤碱基或至少四个相邻的嘌呤碱基。

公开(公告)号：US20080261206A1

公开(公告)日：2008-10-23

申请号：US11574405

申请日：2005-08-28

申请人： Cheol-Min Kim ,  Hee-Kyung Park ,  Eun-Sil Song ,  Jun-Hyung Park ,  Hyun-Jung Jang ,  Byeong-Chul Kang

发明人： Cheol-Min Kim ,  Hee-Kyung Park ,  Eun-Sil Song ,  Jun-Hyung Park ,  Hyun-Jung Jang ,  Byeong-Chul Kang

IPC分类号： C12Q1/68 ,  C40B40/06 ,  C07H21/00

CPC分类号： C12Q1/689 ,  C12Q1/6837

摘要： The present invention relates to a method so called Bacterial Digitalcode System (BaDis) that identifies microorganism by using bacterial-specific, genus-specific and species-specific oligonucleotides from a variety of samples or specimens for detection and differential diagnosis of microorganism. Particularly, the present invention relates to bacterial-specific, genus-specific and species-specific oligonucleotides designed by the target nucleotide sequences of 23S rDNA or ITS gene, polymerase chain reaction (hereinafter, referred to as “PCR”) kits using the oligonucleotides as a primer, the microarray containing the oligonucleotides as a probe, and methods for detecting microorganism by using the oligonucleotides. Therefore, the present invention can be applied to detect the presence of microorganism and diagnose differentially all microorganism such as pathogenic bacteria of infectious diseases, bacteria inducing food poisoning, bacteria contaminating biomedical products and environmental pollutants.

摘要翻译： 本发明涉及一种所谓的细菌数字代码系统（BaDis）的方法，其通过使用来自各种样品或样品的细菌特异性，属特异性和物种特异性寡核苷酸来鉴定微生物，用于微生物的鉴定和鉴别诊断。 特别地，本发明涉及由23S rDNA或ITS基因的靶核苷酸序列设计的细菌特异性，属特异性和物种特异性寡核苷酸，使用寡核苷酸作为聚合酶链反应（以下称为“PCR”）试剂盒 引物，含有寡核苷酸作为探针的微阵列，以及通过使用寡核苷酸检测微生物的方法。 因此，本发明可以应用于检测微生物的存在并差异地诊断所有微生物，如感染性疾病的病原菌，诱导食物中毒的细菌，细菌污染的生物医学产品和环境污染物。

公开(公告)号：US06670130B1

公开(公告)日：2003-12-30

申请号：US09980052

申请日：2001-11-28

申请人： Cheol Min Kim ,  Hee Kyung Park ,  Hyun Jung Jang

发明人： Cheol Min Kim ,  Hee Kyung Park ,  Hyun Jung Jang

IPC分类号： C12Q168

CPC分类号： C07K14/35

摘要： This invention relates to oligonucleotides sequence of probes or primers for detection or identication of Mycobacterium. In the claimed invention, oligonucleotide sequences of ITS (Internal Transcribing Spacer Region) from M. fortuitium, M. chelonae, M. abscessus, M. vaccae, M. flavescence, M. Asiaticum, M. porcinum, M. acapulcensis and M. diernhoferi have been identified. Using these ITS sequences, PCR primers or hybridization probes for detection or identication of Mycobacterium have been developed and presented as seq ID: 10 to seq ID: 241.

摘要翻译： 本发明涉及用于检测或鉴定分枝杆菌的探针或引物的寡核苷酸序列。 在要求保护的发明中，来自M.fusuitium，M.crcaeaeae，M.paepusus，M. vaccae，M.flavescence，M. Asiaticum，M.polcinum，M.Acapulcensis和M.的ITS（内部转录间隔区）的寡核苷酸序列。 diernhoferi已被确定。 使用这些ITS序列，已经开发了用于检测或鉴定分枝杆菌的PCR引物或杂交探针，并以SEQ ID NO：10至SEQ ID NO：241表示。