公开(公告)号：US20090286691A1

公开(公告)日：2009-11-19

申请号：US12161691

申请日：2006-01-20

申请人： Cheol-Min Kim ,  Hee-Kyung Park ,  Hyun-Jung Jang ,  Eun-Sil Song

发明人： Cheol-Min Kim ,  Hee-Kyung Park ,  Hyun-Jung Jang ,  Eun-Sil Song

IPC分类号： C40B30/04 ,  C07H21/04 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/08

CPC分类号： C12Q1/689

摘要： The present invention relates to oligonucleotides for detection of sepsis-causing bacteria and a detection method using the oligonucleotides, more particularly to a microarry comprising at least one of gram positive bacteria-specific and gram negative bacteria-specific oligonucleotides, sepsis-causing bacteria's genus-specific and species-specific oligonucleotides designed from the ITS target region which is hypervariable base sequence of sepsis-causing bacteria as probes, and a detection method and a diagnosis kit by using the same.According to the present invention, the present invention can provide an antibiotics therapy for accurately removing infectious agent related to sepsis by detecting existence of sepsis-causing bacteria and identifying gram positive- and gram negative-bacteria and genus and species of the bacteria, at once. And, the present invention can prevent a patient from abuse and misuse of antibiotics and decrease time of hospital treatment and medical cost of the patient. Further, the present invention has advantage of preventing complications and reducing mortality rate.

摘要翻译： 本发明涉及用于检测败血症细菌的寡核苷酸和使用寡核苷酸的检测方法，更具体地涉及包含革兰氏阳性细菌特异性和革兰氏阴性细菌特异性寡核苷酸中的至少一种的微阵列，败血症细菌的属 - 由作为败血症的细菌作为探针的高变碱基序列的ITS靶区设计的特异性和物种特异性寡核苷酸，以及使用该检测方法和诊断试剂盒。 根据本发明，本发明可以提供一种用于通过检测脓毒症引起的细菌的存在并一次鉴定革兰氏阳性和革兰氏阴性细菌以及细菌的种类和物种来精确地去除与败血症相关的传染原的抗生素治疗 。 并且，本发明可以防止患者滥用和滥用抗生素，并减少患者的医院治疗时间和医疗费用。 此外，本发明具有防止并发症和降低死亡率的优点。

公开(公告)号：US20070065828A1

公开(公告)日：2007-03-22

申请号：US10597173

申请日：2005-01-14

申请人： Cheol-Min Kim ,  Hee-Kyung Park ,  Hyun-Jung Jang ,  Hyo-Myoung Kim

发明人： Cheol-Min Kim ,  Hee-Kyung Park ,  Hyun-Jung Jang ,  Hyo-Myoung Kim

IPC分类号： C12Q1/68 ,  C12M3/00

CPC分类号： C12Q1/689

摘要： The present invention relates to a method for detecting Mycoplasma and its related strains which are source of contamination of cell lines and biological products and human pathogenic. More particularly, the present invention relates to genus-specific and species-specific oligonucleotides for genotyping of Mycoplasma, Acholeplasm and Ureaplasma strains, microarray comprising the oligonucleotides, and method for detection of species using the microarray. As described above, the present invention provides a rapid and accurate assay method capable of simultaneously detecting many Mycoplasma and its related strains from a single sample using a microarray comprising novel oligonucleotides for detecting Mycoplasma and its related strains which are known as a source of contamination of cell lines and biological products and human pathogenic. Further, the present invention provides an objective and credible assay method capable of tracing a contamination source for preventing expansion of infective Mycoplasma and its related strains and controlling a contamination of Mycoplasma against biological products and stem cells or cord blood cells which are useful for gene therapy and cell therapy.

摘要翻译： 本发明涉及一种检测支原体及其相关菌株的方法，这些菌株是细胞系和生物制品的污染源和人类致病菌。 更具体地说，本发明涉及用于基因分型支原体，痘苗和解脲支原体菌株的特异性和物种特异性寡核苷酸，包含寡核苷酸的微阵列，以及使用微阵列检测物种的方法。 如上所述，本发明提供了一种快速且准确的测定方法，其能够使用包含用于检测支原体及其相关菌株的微阵列的微阵列同时检测许多支原体及其相关菌株，所述新型寡核苷酸及其相关菌株被称为污染源 细胞系和生物制品和人类致病性。 此外，本发明提供了一种客观且可靠的测定方法，其能够追踪污染源以防止感染性支原体及其相关菌株的扩增，并控制支原体对生物制品和干细胞或脐带血细胞的污染，这些基因治疗可用于基因治疗 和细胞治疗。

公开(公告)号：US20080261206A1

公开(公告)日：2008-10-23

申请号：US11574405

申请日：2005-08-28

申请人： Cheol-Min Kim ,  Hee-Kyung Park ,  Eun-Sil Song ,  Jun-Hyung Park ,  Hyun-Jung Jang ,  Byeong-Chul Kang

发明人： Cheol-Min Kim ,  Hee-Kyung Park ,  Eun-Sil Song ,  Jun-Hyung Park ,  Hyun-Jung Jang ,  Byeong-Chul Kang

IPC分类号： C12Q1/68 ,  C40B40/06 ,  C07H21/00

CPC分类号： C12Q1/689 ,  C12Q1/6837

摘要： The present invention relates to a method so called Bacterial Digitalcode System (BaDis) that identifies microorganism by using bacterial-specific, genus-specific and species-specific oligonucleotides from a variety of samples or specimens for detection and differential diagnosis of microorganism. Particularly, the present invention relates to bacterial-specific, genus-specific and species-specific oligonucleotides designed by the target nucleotide sequences of 23S rDNA or ITS gene, polymerase chain reaction (hereinafter, referred to as “PCR”) kits using the oligonucleotides as a primer, the microarray containing the oligonucleotides as a probe, and methods for detecting microorganism by using the oligonucleotides. Therefore, the present invention can be applied to detect the presence of microorganism and diagnose differentially all microorganism such as pathogenic bacteria of infectious diseases, bacteria inducing food poisoning, bacteria contaminating biomedical products and environmental pollutants.

摘要翻译： 本发明涉及一种所谓的细菌数字代码系统（BaDis）的方法，其通过使用来自各种样品或样品的细菌特异性，属特异性和物种特异性寡核苷酸来鉴定微生物，用于微生物的鉴定和鉴别诊断。 特别地，本发明涉及由23S rDNA或ITS基因的靶核苷酸序列设计的细菌特异性，属特异性和物种特异性寡核苷酸，使用寡核苷酸作为聚合酶链反应（以下称为“PCR”）试剂盒 引物，含有寡核苷酸作为探针的微阵列，以及通过使用寡核苷酸检测微生物的方法。 因此，本发明可以应用于检测微生物的存在并差异地诊断所有微生物，如感染性疾病的病原菌，诱导食物中毒的细菌，细菌污染的生物医学产品和环境污染物。

公开(公告)号：US06670130B1

公开(公告)日：2003-12-30

申请号：US09980052

申请日：2001-11-28

申请人： Cheol Min Kim ,  Hee Kyung Park ,  Hyun Jung Jang

发明人： Cheol Min Kim ,  Hee Kyung Park ,  Hyun Jung Jang

IPC分类号： C12Q168

CPC分类号： C07K14/35

摘要： This invention relates to oligonucleotides sequence of probes or primers for detection or identication of Mycobacterium. In the claimed invention, oligonucleotide sequences of ITS (Internal Transcribing Spacer Region) from M. fortuitium, M. chelonae, M. abscessus, M. vaccae, M. flavescence, M. Asiaticum, M. porcinum, M. acapulcensis and M. diernhoferi have been identified. Using these ITS sequences, PCR primers or hybridization probes for detection or identication of Mycobacterium have been developed and presented as seq ID: 10 to seq ID: 241.

摘要翻译： 本发明涉及用于检测或鉴定分枝杆菌的探针或引物的寡核苷酸序列。 在要求保护的发明中，来自M.fusuitium，M.crcaeaeae，M.paepusus，M. vaccae，M.flavescence，M. Asiaticum，M.polcinum，M.Acapulcensis和M.的ITS（内部转录间隔区）的寡核苷酸序列。 diernhoferi已被确定。 使用这些ITS序列，已经开发了用于检测或鉴定分枝杆菌的PCR引物或杂交探针，并以SEQ ID NO：10至SEQ ID NO：241表示。

公开(公告)号：US20060134792A1

公开(公告)日：2006-06-22

申请号：US10567072

申请日：2004-08-02

申请人： Cheol-Min Kim ,  Hee-Kyung Park ,  Mong Cho ,  Hyun-Jung Jang ,  Jeong Heo

发明人： Cheol-Min Kim ,  Hee-Kyung Park ,  Mong Cho ,  Hyun-Jung Jang ,  Jeong Heo

IPC分类号： C12Q1/00

CPC分类号： C12Q1/706 ,  C12Q1/6837 ,  C12Q2545/113

摘要： Provided are a microarray manufactured using a mixture of target probes for drug-resistant HBV detection, quality control probes for controlling quality in probe hybridization and fabrication of microarrays, and negative control probes for determining the presence and ratio of more than one type, i.e., a wild type and a mutant in a codon, measuring a background of non-specific cross-hybridization, and discriminating homozygotes and heterozygotes, and a method of detecting a drug-resistant HBV, controlling the quality of a microarray, determining the presence and ratio of more than one type, and determining positive and false positive probes at the same time using the microarray. The microarray, which includes the target probes for drug-resistant HBV detection, the QC probes, and the negative control probes, can detect a drug-resistant HBV, control quality in fabrication of microarrays and hybridization, determine the presence and ratio of more than one type, i.e., a wild type and a mutant, determine positive and false positive probes by measuring a background of non-specific cross-hybridization, and discriminate homozygotes and heterozygotes. When a plurality of sets of probes, each set containing target probes, QC probes, and negative control probes, are immobilized on a support of the microarray, detection of resistance in HBV to multiple drugs, quality control, and determination as to the presence and ratio of a wild type and a mutant and whether each probe is positive or false positive can be rapidly and accurately performed.

摘要翻译： 提供了使用用于耐药HBV检测的靶探针混合物制造的微阵列，用于控制探针杂交质量的控制探针和微阵列的制造以及用于确定多于一种类型的存在和比例的阴性对照探针， 密码子中的野生型和突变体，测量非特异性交叉杂交的背景，以及鉴别纯合子和杂合子，以及检测耐药性HBV的方法，控制微阵列的质量，确定存在和比例 的多种类型，并使用微阵列同时确定阳性和假阳性探针。 包括用于耐药HBV检测的目标探针，QC探针和阴性对照探针的微阵列可以检测耐药性HBV，控制微阵列和杂交的制备质量，确定多于一个的存在和比例 一种类型，即野生型和突变体，通过测量非特异性交叉杂交的背景，并鉴别纯合子和杂合子来确定阳性和假阳性探针。 当将包含目标探针，QC探针和阴性对照探针的多组探针固定在微阵列的载体上时，检测HBV对多种药物的耐药性，质量控制和确定存在和 野生型和突变体的比例以及每种探针是阳性还是假阳性可以快速和准确地进行。