公开(公告)号：US5494810A

公开(公告)日：1996-02-27

申请号：US343785

申请日：1994-11-22

申请人： Francis Barany ,  John Zebala ,  Deborah Nickerson ,  Robert J. Kaiser, Jr. ,  Leroy Hood

发明人： Francis Barany ,  John Zebala ,  Deborah Nickerson ,  Robert J. Kaiser, Jr. ,  Leroy Hood

IPC分类号： C12N9/00 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C12Q1/25

CPC分类号： C12Q1/6862 ,  C12N15/10 ,  C12N9/93 ,  C12Q1/6813 ,  C12Q1/6858

摘要： The present invention relates to the cloning of the gene of a thermophilic DNA ligase, from Thermus aquaticus strain HB8, and the use of this ligase in a ligase chain reaction (LCR) assay for the detection of specific sequences of nucleotides in a variety of nucleic acid samples, and more particularly in those samples containing a DNA sequence characterized by a difference in the nucleic acid sequence from a standard sequence including single nucleic acid base pair changes, deletions, insertions or translocations.

摘要翻译： 本发明涉及从水生栖热菌菌株HB8的嗜热DNA连接酶的基因的克隆，以及该连接酶在连接酶链反应（LCR）测定中用于检测多种核酸中特定的核苷酸序列的用途 更特别是含有DNA序列的那些样品，其特征在于核酸序列与包括单个核酸碱基对变异，缺失，插入或易位的标准序列的差异。