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    DETECTION OF BRAIN CANCER TYPES
    2.
    发明申请
    DETECTION OF BRAIN CANCER TYPES 审中-公开
    检测脑癌类型

    公开(公告)号：US20150252429A1

    公开(公告)日：2015-09-10

    申请号：US14439974

    申请日：2013-10-31

    申请人： Nathan D. PRICE ,  Leroy HOOD ,  Jaeyun SUNG ,  Donald GEMAN ,  INSTITUTE FOR SYSTEMS BIOLOGY

    发明人： Nathan D. Price ,  Leroy Hood ,  Jaeyun Sung ,  Donald Geman

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16

    摘要： The invention provides methods to identify various types of brain cancer tissue by comparing gene expression transcriptomes in tissue samples. A sequential method to discriminate among six different types of brain cancer is described. The invention relates to the field of markers for various types of brain cancer. More particularly, it relies on a sequential system for sorting individual cancer types.

    摘要翻译： 本发明提供了通过比较组织样品中的基因表达转录组来鉴定各种类型的脑癌组织的方法。 描述了区分六种不同类型的脑癌的顺序方法。 本发明涉及各种脑癌的标志物领域。 更具体地，它依赖于用于分类各种癌症类型的顺序系统。

    Organ-specific proteins and methods of their use
    3.
    发明授权
    Organ-specific proteins and methods of their use 有权
    器官特异性蛋白质及其使用方法

    公开(公告)号：US08586006B2

    公开(公告)日：2013-11-19

    申请号：US12376951

    申请日：2007-08-09

    申请人： Leroy Hood ,  Patricia M. Beckmann ,  Richard Johnson ,  Marcello Marelli ,  Xiaojun Li

    发明人： Leroy Hood ,  Patricia M. Beckmann ,  Richard Johnson ,  Marcello Marelli ,  Xiaojun Li

    IPC分类号： A61K49/00

    CPC分类号： G01N33/68 ,  G01N33/6845 ,  G01N2800/00 ,  G01N2800/52

    摘要： The present invention relates generally to methods for identifying and using organ-specific proteins and transcripts. The present invention further provides compositions comprising organ-specific proteins and transcripts encoding the same, detection reagents for detecting such proteins and transcripts, and diagnostic panels, kits and arrays for measuring organ-specific proteins/transcripts in blood, biological tissue or other biological fluid.

    摘要翻译： 本发明一般涉及鉴定和使用器官特异性蛋白质和转录物的方法。 本发明还提供了包含器官特异性蛋白质和编码其的转录物的组合物，用于检测这些蛋白质和转录物的检测试剂，以及用于测量血液，生物组织或其他生物流体中的器官特异性蛋白质/转录物的诊断板，试剂盒和阵列 。

    Plasma membrane biomarkers preferentially expressed in pancreatic beta cells useful in imaging or targeting beta cells
    4.
    发明授权
    Plasma membrane biomarkers preferentially expressed in pancreatic beta cells useful in imaging or targeting beta cells 有权
    血浆膜生物标志物优先在用于成像或靶向β细胞的胰腺β细胞中表达

    公开(公告)号：US08425878B2

    公开(公告)日：2013-04-23

    申请号：US12735731

    申请日：2009-02-13

    申请人： Decio L. Eizirik ,  Daisy Flamez ,  Isabelle Salmon ,  Alix Berton ,  Isabelle Roland ,  Daniel Marechal ,  Marie-Claire Beckers ,  Leroy Hood ,  Nathan Goodman ,  Burak Kutlu

    发明人： Decio L. Eizirik ,  Daisy Flamez ,  Isabelle Salmon ,  Alix Berton ,  Isabelle Roland ,  Daniel Marechal ,  Marie-Claire Beckers ,  Leroy Hood ,  Nathan Goodman ,  Burak Kutlu

    IPC分类号： A61K49/00

    CPC分类号： G01N33/573 ,  C07K14/705 ,  G01N33/57438 ,  G01N2800/042

    摘要： The present invention is directed to the identification of a biomarker specifically located in the plasma membrane of pancreatic beta cells. It was selected by a Systems Biology approach on Massively Parallel Signal Sequencing datasets obtained in human islets and Affymetrix microarray datasets on human islets, purified rat primary beta and non beta cells and insulinoma cells. Based on a set of specific features the biomarker is a unique candidate for imaging and targeting strategies to study the pancreatic beta cell mass in health and disease (T1 D, T2D, pancreatic cancers, obesity, islet transplantation, beta cell regeneration). The five specific features of the selected biomarkers are: 1) Preferentially expressed in pancreatic islets as compared to surrounding tissues; 2) Higher expression in pancreatic beta cells than in pancreatic alpha cells or than in other islet non-beta cells; 3) Expression levels in pancreatic beta cells are higher or comparable to glucokinase which is an enzyme specifically expressed in the pancreatic beta cell; 4) Located in the membrane and as such targetable with antibodies, peptides or small molecules which allows imaging, targeting and immunohistochemistry; and 5) Expression is not induced during the process of inflammation of the beta cell mass and the protein is not enriched in T-cells and dendritic cells or in other cells participating in the inflammation process.

    摘要翻译： 本发明涉及特异性位于胰β细胞质膜中的生物标志物的鉴定。 它是通过系统生物学方法在人胰岛获得的大规模并行信号测序数据集和人胰岛，纯化的大鼠原代β和非β细胞和胰岛素瘤细胞的Affymetrix微阵列数据集中选择的。 基于一组具体特征，生物标志物是研究胰腺β细胞在健康和疾病（T1D，T2D，胰腺癌，肥胖，胰岛移植，β细胞再生）中成像和靶向策略的独特候选。 所选生物标志物的五个特征是：1）与周围组织相比，胰岛中优先表达; 2）胰腺β细胞中的表达高于胰腺α细胞或其他胰岛β细胞; 3）胰腺β细胞中的表达水平较高，或与胰腺β细胞特异性表达的酶的葡萄糖激酶相当; 4）位于膜中并且因此可靶向抗体，肽或小分子，其允许成像，靶向和免疫组织化学; 和5）在β细胞质量的炎症过程中不诱导表达，并且蛋白质不富含T细胞和树突状细胞或参与炎症过程的其它细胞中。

    Methods for haplotyping genomic DNA
    5.
    发明授权
    Methods for haplotyping genomic DNA 有权
    单倍型基因组DNA的方法

    公开(公告)号：US07972791B2

    公开(公告)日：2011-07-05

    申请号：US12569044

    申请日：2009-09-29

    申请人： Effie W. Petersdorf ,  Zhen Guo ,  Leroy Hood

    发明人： Effie W. Petersdorf ,  Zhen Guo ,  Leroy Hood

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6813

    摘要： The present invention provides a novel method for specifically isolating and separating large segments of genomic DNA that can subsequently be used to determine a genomic haplotype. The invention relies on using a solid phase having a flat surface arrayed with oligonucleotides designed to specifically hybridize to each particular haplotype of an individual sample, e.g., oligonucleotides designed to specifically hybridize with each of the two HLA-B haplotypes, HLA-A, HLA-C, HLA-DR, HLA-DQ, and the like. The genomic DNA is contacted and hybridized to the arrayed oligonucleotides to form a genomic DNA/oligonucleotide complex. The excess genomic DNA is washed away and the haplotype separated genomic DNA is denatured from the oligonucleotide probe and collected. The method of the present invention allows for the separation of genomic DNA fragments of between approximately 2 to about 4 megabases (Mb). Separation of the haplotypes of large genomic DNA fragments allows for linkage analysis of other HLA alleles and polymorphisms, microsatellite, SNPs, and the like across a large span of the HLA region, including HLA-A, -B, -C, and HLA-DRB1 regions. This linkage analysis is particularly useful when HLA typing for an individual with limited family HLA typing available.

    摘要翻译： 本发明提供了用于特异性分离和分离基因组DNA的大片段的新方法，其随后可用于确定基因组单倍型。 本发明依赖于使用具有平面的固相，其具有设计成与单个样品的每个特定单体型特异性杂交的寡核苷酸排列的寡核苷酸，例如设计用于与两种HLA-B单倍体HLA-A，HLA -C，HLA-DR，HLA-DQ等。 将基因组DNA与排列的寡核苷酸接触并杂交以形成基因组DNA /寡核苷酸复合物。 洗去多余的基因组DNA，并将单体型分离的基因组DNA从寡核苷酸探针中变性并收集。 本发明的方法允许分离约2至约4兆碱基（Mb）之间的基因组DNA片段。 分离大型基因组DNA片段的单倍型允许跨越HLA区域的大跨度，包括HLA-A，-B，-C和HLA-的其他HLA等位基因和多态性，微卫星，SNP等的连锁分析。 DRB1区域。 当使用有限家庭HLA分型的个体进行HLA分型时，这种连锁分析特别有用。

    METHODS FOR IDENTIFYING AND MONITORING DRUG SIDE EFFECTS
    6.
    发明申请
    METHODS FOR IDENTIFYING AND MONITORING DRUG SIDE EFFECTS 有权
    鉴别和监测药物副作用的方法

    公开(公告)号：US20110136690A1

    公开(公告)日：2011-06-09

    申请号：US13023366

    申请日：2011-02-08

    申请人： LEROY HOOD ,  BIAOYANG LIN

    发明人： LEROY HOOD ,  BIAOYANG LIN

    IPC分类号： G01N33/68 ,  G01N33/53 ,  C40B30/04

    CPC分类号： G01N33/57434 ,  G01N33/6842 ,  G01N33/6893

    摘要： The present invention relates generally to methods for identifying drug side effects by detecting perturbations in organ-specific molecular blood fingerprints. The invention further relates to methods for identifying drug-specific organ-specific molecular blood fingerprints. As such, the present invention provides compositions comprising organ-specific proteins, detection reagents for detecting such proteins, and panels and arrays for determining organ-specific molecular blood fingerprints.

    摘要翻译： 本发明一般涉及通过检测器官特异性分子血液指纹图中的扰动来鉴定药物副作用的方法。 本发明还涉及用于鉴定药物特异性器官特异性分子血液指纹图谱的方法。 因此，本发明提供了包含器官特异性蛋白质的组合物，用于检测这些蛋白质的检测试剂，以及用于测定器官特异性分子血液指纹图谱和阵列。

    Thermostable polymerases having altered fidelity and methods of identifying and using same
    10.
    发明授权
    Thermostable polymerases having altered fidelity and methods of identifying and using same 有权
    具有改变的保真度的热稳定性聚合酶以及鉴定和使用它们的方法

    公开(公告)号：US07312059B2

    公开(公告)日：2007-12-25

    申请号：US11096645

    申请日：2005-03-31

    申请人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    发明人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    IPC分类号： C12N9/10 ,  C07K1/00

    CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

    摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

    摘要翻译： 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如，本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸，例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。