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公开(公告)号：US09165359B2
公开(公告)日：2015-10-20
申请号：US14595693
申请日：2015-01-13
申请人： Rajiv Bharadwaj , Bahram Fathollahi
发明人： Rajiv Bharadwaj , Bahram Fathollahi
CPC分类号： H04N5/2251 , B01L3/502761 , B01L2200/0636 , B01L2200/0652 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , C12M47/04 , C12N5/0081 , C12N5/0087 , G02B21/365 , G06T7/0012 , H04N5/2256 , H04N7/18
摘要： The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.
摘要翻译： 本发明提供了一种隔离单个靶细胞的方法和装置。 该装置包括主体结构，其包括主通道，收集通道和在第一流体结处流体耦合的废物通道。 多个捕获通道与收集通道相交，每个捕获通道在与捕集通道与收集通道的交叉点相邻的位置处的直径小于单个靶细胞的直径。 该装置还包括：成像系统，被配置为对主通道内的各个目标单元和非目标单元进行成像，从而产生成像数据; 处理器，被配置为执行所述成像数据的实时，多变量分析; 以及被配置为引导各个目标细胞的引导系统。 压力源与收集通道，废物通道，第一侧通道和第二侧通道中的一个或多个流体连通。
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公开(公告)号：US20120258487A1
公开(公告)日：2012-10-11
申请号：US13437727
申请日：2012-04-02
CPC分类号： C12N15/1027 , C12N15/10 , C12N15/66
摘要： This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of said biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to for a sequenced order of droplets carrying different components of said biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of said biological construct to permit said components to react with each other in one or more reactions contributing to the assembly of said biological construct.
摘要翻译： 本发明提供用于组装生物构建体（例如质粒，转化细胞等）的方法和组合物。 在某些实施方案中，所述方法包括将所述生物构建体的单独组分封装在限制在流体通道中的流体液滴中; 任选地将来自不同流体通道的液滴混合以便在通道或室中携带所述生物构建体的不同组分的液滴的顺序排列; 并且任选地组合两个或更多个液滴，每个液滴包含所述生物构建体的不同组分，以允许所述组分在有助于所述生物构建体的组装的一个或多个反应中彼此反应。
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