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17 条记录 (耗时 0.017 秒)

    Method for purifying taxol from taxus biomass
    14.
    发明授权
    Method for purifying taxol from taxus biomass 失效
    紫杉醇从紫杉醇生物质中提纯的方法

    公开(公告)号：US5900367A

    公开(公告)日：1999-05-04

    申请号：US652493

    申请日：1996-05-28

    申请人： Seung-Suh Hong ,  Bong-Kyu Song ,  Jin-Hyun Kim ,  Chang-Bae Lim ,  Hyun-Soo Lee ,  Kwang-Wook Kim ,  In-Seon Kang ,  Hung-Bok Park

    发明人： Seung-Suh Hong ,  Bong-Kyu Song ,  Jin-Hyun Kim ,  Chang-Bae Lim ,  Hyun-Soo Lee ,  Kwang-Wook Kim ,  In-Seon Kang ,  Hung-Bok Park

    IPC分类号： C12P15/00 ,  C12P17/02 ,  C12R1/91 ,  A61K35/78

    CPC分类号： C12P17/02

    摘要： The present invention relates to a rapid and simple method for mass production of taxol from the cell culture of Taxus genus plant with a high purity and recovery. The method for mass production of taxol from Taxus genus plant of the present invention, comprises the steps of: (i) organic solvent extraction of biomass from Taxus genus plant to obtain a crude extract; (ii) synthetic adsorbent treatment of the crude extract and filtration to give filtrate; (iii) addition of hexane to the filtrate to precipitate crude taxol; (iv) fractional precipitation of the crude taxol in a mixture of alcohol and water and vacuum drying the precipitate to obtain taxol powder; and, (v) high performance liquid chromatography of the taxol powder. According to the method of the present invention, taxol of over 99% purity can be simply obtained from Taxus genus plant with a high recovery of over 90%.

    摘要翻译： PCT No.PCT / KR96 / 00059 Sec。 371日期：1996年5月28日 102（e）日期1996年5月28日PCT提交1996年4月27日PCT公布。 公开号WO96 / 34973 日期1996年11月7日本发明涉及一种高纯度和回收率的紫杉属植物细胞培养物中大量生产紫杉醇的快速简便方法。 本发明紫杉属植物紫杉醇的批量生产方法，包括以下步骤：（一）从紫杉属植物提取生物质的有机溶剂，得到粗提物; （ii）合成吸附剂处理粗提物并过滤得到滤液; （iii）向滤液中加入己烷以沉淀粗紫杉醇; （iv）将粗紫杉醇在醇和水的混合物中分级沉淀，并真空干燥沉淀物以获得紫杉醇粉末; 和（v）紫杉醇粉末的高效液相色谱法。 根据本发明的方法，可以简单地从具有90％以上高回收率的紫杉属植物获得纯度超过99％的紫杉醇。

    Mass production of paclitaxel by changing the temperature of the medium during the plant cell culture
    15.
    发明授权
    Mass production of paclitaxel by changing the temperature of the medium during the plant cell culture 有权
    通过在植物细胞培养期间改变培养基的温度来批量生产紫杉醇

    公开(公告)号：US06589765B1

    公开(公告)日：2003-07-08

    申请号：US09269154

    申请日：1999-02-26

    申请人： Ho-Joon Choi ,  Hyung-Kyoon Choi ,  Sang-Ic Kim ,  Jeong-Hwan Yun ,  Joo-Sun Son ,  Moon-Seok Chang ,  Eunsoo Choi ,  Hong-Rak Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Ho-Joon Choi ,  Hyung-Kyoon Choi ,  Sang-Ic Kim ,  Jeong-Hwan Yun ,  Joo-Sun Son ,  Moon-Seok Chang ,  Eunsoo Choi ,  Hong-Rak Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： C12P1702

    CPC分类号： C12N5/04 ,  C12P17/02

    摘要： The present invention relate to a method to increase the production level of paclitaxel by changing the temperature during the plant cell culture. According to the present invention, the production of the paclitaxel comprises the following procedure: (i) cultivating the Taxus genus plant cells in a medium at ca. 20 to 25° C.; and (ii) when growth of the plant cells has progressed sufficiently, changing the cultivation temperature to ca. 26 to 32° C. to continue the culture. The present invention comprises also the method of increasing the paclitaxel production by inoculating the cells at a high initial concentration and by increasing the saccharide concentration in the medium: According to the present invention, paclitaxel can be mass-produced conveniently and therefore has an industrial application.

    摘要翻译： 本发明涉及通过改变植物细胞培养过程中的温度来提高紫杉醇生产水平的方法。 根据本发明，紫杉醇的制备包括以下步骤：（i）在培养基中培养紫杉属植物细胞。 20至25℃。 和（ii）当植物细胞生长充分进展时，将培养温度改变为约 26至32°C继续培养。 本发明还包括通过以高初始浓度接种细胞并增加培养基中的糖浓度来增加紫杉醇产生的方法：根据本发明，紫杉醇可以方便批量生产，因此具有工业应用 。

    Cell growth inhibiting and cell differentiation specific SYG972 gene, genomic DNA and promoter thereof
    16.
    发明授权
    Cell growth inhibiting and cell differentiation specific SYG972 gene, genomic DNA and promoter thereof 失效
    细胞生长抑制和细胞分化特异性SYG972基因，基因组DNA及其启动子

    公开(公告)号：US06573371B2

    公开(公告)日：2003-06-03

    申请号：US09879833

    申请日：2001-06-12

    申请人： Neon-Cheol Jung ,  Jin-Man Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee ,  Young-Rim Seong ,  Jae-Ho Lee

    发明人： Neon-Cheol Jung ,  Jin-Man Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee ,  Young-Rim Seong ,  Jae-Ho Lee

    IPC分类号： C07H2102

    CPC分类号： C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158

    摘要： The present invention relates to a diagnosis method of cancer using SYG972 gene and to the genomic DNA and to a promoter regulating transcription of the gene. Expression of SYG972 gene is greatly reduced in breast cancer cells compared to normal breast cells. Therefore the present invention provides a method of diagnosis method by using the gene of the present invention or its fragments as a probe. SYG972 genomic according to the present invention is composed of 4 exons and 3 introns. Promoter is 3 kb long from the transcription initiating site in the 5′ direction. SYG972 genomic DNA and promoter can be used to design and screen drugs to promote or to inhibit apoptosis and differentiation of cells, especially to screen drugs to treat cancer, a disease wherein cell growth and differentiation is abnormal.

    摘要翻译： 本发明涉及使用SYG972基因和基因组DNA的癌症诊断方法以及调节基因转录的启动子。 与正常乳腺细胞相比，乳腺癌细胞中SYG972基因的表达大大降低。 因此，本发明提供了通过使用本发明的基因或其片段作为探针的诊断方法。 根据本发明的SYG972基因组由4个外显子和3个内含子组成。 启动子距5'方向的转录起始位点长3 kb。 SYG972基因组DNA和启动子可用于设计和筛选药物，以促进或抑制细胞的凋亡和分化，特别是筛选药物以治疗癌症，其中细胞生长和分化异常。

    Method for treating rheumatoid arthritis with composition containing histone
    17.
    发明授权
    Method for treating rheumatoid arthritis with composition containing histone 有权
    用含有组蛋白的组合物治疗类风湿性关节炎的方法

    公开(公告)号：US06204242B1

    公开(公告)日：2001-03-20

    申请号：US09381559

    申请日：2000-01-19

    申请人： Insoo Bae ,  Dong-soo Kim ,  Heajoon Yim ,  Neon-Cheol Jung ,  Yong-Weon Yi ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Insoo Bae ,  Dong-soo Kim ,  Heajoon Yim ,  Neon-Cheol Jung ,  Yong-Weon Yi ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： A61K3800

    CPC分类号： A61K38/1709

    摘要： The present invention relates to a novel use of histone and provides a pharmaceutical composition containing histone as an active ingredient to improve the symptoms of progressive, inflammatory and autoimmune arthritis. The pharmaceutical composition of the present invention includes histone, especially histone H1 as an active ingredient, and could include pharmacologically approved carriers if necessary. Histone H1 lowered induction of arthritis and reduced arthritis index more effectively than steroidal dexamethasone and also had a significant preventive effect.

    摘要翻译： 本发明涉及组蛋白的新用途，并提供含有组蛋白作为活性成分以改善进行性，炎性和自身免疫性关节炎症状的药物组合物。 本发明的药物组合物包括组蛋白，特别是组蛋白H1作为活性成分，如果需要，可以包括药理学上认可的载体。 组蛋白H1比甾体地塞米松更有效地降低了关节炎的诱导，降低了关节炎指数，并且具有显着的预防作用。