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公开(公告)号：US20080182263A1
公开(公告)日：2008-07-31
申请号：US12022079
申请日：2008-01-29
Applicant: Stephen J. Gunstream
Inventor： Stephen J. Gunstream
CPC classification number: C12Q1/6851 , G01N21/274 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2021/6441 , G01N2201/12 , G01N2201/12746 , G16B30/00 , C12Q2545/113 , C12Q2545/101
Abstract: The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (RT-PCR) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same RT-PCR thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample. A set of maxima values and a set of minimum values, and/or other calibration information useful for adjusting emission data for sample dyes can be recorded, for example, for 10 cycles, 20 cycles, or each cycle of a complete RT-PCR run. Such testing of dye response under realistic operating conditions can enable more accurate characterization of plate, dye, filter, or instrument response and therefore more accurate calibration corrections and other and/or adjustments.
Abstract translation: 本教导涉及在实时聚合酶链反应（RT-PCR）或其他扩增反应期间产生校准信息的方法。 样品孔板或其它载体可以含有一种或多种经受相同RT-PCR热循环或用于在生物样品上进行扩增或其他反应的其他条件的染料或其它参考物质。 可以记录一组最大值和一组最小值，和/或可用于调整样品染料的发射数据的其他校准信息，例如10个循环，20个循环或完整RT-PCR运行的每个循环 。 在真实的操作条件下对染料响应的这种测试可以实现板，染料，过滤器或仪器响应的更精确的表征，因此可以进行更精确的校准校正和其他和/或调整。
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公开(公告)号：US20080178653A1
公开(公告)日：2008-07-31
申请号：US12022094
申请日：2008-01-29
Applicant: Stephen J. Gunstream
Inventor： Stephen J. Gunstream
IPC: G01N37/00
CPC classification number: C12Q1/6851 , G01N21/274 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2021/6441 , G01N2201/12 , G01N2201/12746 , G16B30/00 , C12Q2545/113 , C12Q2545/101
Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.
Abstract translation: 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。
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13.发明授权公开(公告)号：US08265883B2
公开(公告)日：2012-09-11
申请号：US13070273
申请日：2011-03-23
Applicant: Stephen J. Gunstream
Inventor： Stephen J. Gunstream
IPC: G06F19/00
CPC classification number: C12Q1/6851 , G01N21/274 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2021/6441 , G01N2201/12 , G01N2201/12746 , G06F19/22 , C12Q2545/113 , C12Q2545/101
Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.
Abstract translation: 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。
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Patent Agency Ranking
公开(公告)号：US20120160995A1
公开(公告)日：2012-06-28
申请号：US13302793
申请日：2011-11-22
Applicant: Stephen J. GUNSTREAM , Mark Oldham
Inventor： Stephen J. GUNSTREAM , Mark Oldham
IPC: G01J1/58
CPC classification number: G01J1/58 , G01N21/278 , G01N21/6452 , Y10T436/10 , Y10T436/11 , Y10T436/112499 , Y10T436/113332
Abstract: Implementations of the present invention describe an apparatus for generating calibration factors for a spectral detector instrument. The calibration factors are derived from a calibration plate containing one or more spectral species in each well of the calibration plate. Each well is then exposed to an excitation source that causes the one or more spectral species in each of the wells to fluoresce. The signal response is measured and associated with each spectral species at each different well position in the calibration plate. Next, the measured signal response from each spectral species at each well position in the calibration plate is compared with a predetermined signal response for each spectral species. The results of this comparison can be used to determine a calibration factor for each well and spectral species to compensate for the difference between the measured signal response and the predetermined signal response.
Abstract translation: 本发明的实施方式描述了一种用于产生光谱检测器仪器的校准因子的装置。 校准因子来源于校准板的每个孔中含有一个或多个光谱种类的校准板。 然后将每个孔暴露于激发源，使得每个孔中的一个或多个光谱物质发荧光。 测量信号响应并与校准板中每个不同孔位置处的每个光谱种类相关联。 接下来，将来自校准板中每个孔位置处的每个光谱种类的测量信号响应与每个光谱种类的预定信号响应进行比较。 该比较的结果可用于确定每个孔和光谱种类的校准因子，以补偿测量的信号响应与预定信号响应之间的差异。
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公开(公告)号：US08084260B2
公开(公告)日：2011-12-27
申请号：US11286071
申请日：2005-11-23
Applicant: Stephen J. Gunstream , Mark Oldham
Inventor： Stephen J. Gunstream , Mark Oldham
CPC classification number: G01J1/58 , G01N21/278 , G01N21/6452 , Y10T436/10 , Y10T436/11 , Y10T436/112499 , Y10T436/113332
Abstract: Implementations of the present invention describe an apparatus for generating calibration factors for a spectral detector instrument. The calibration factors are derived from a calibration plate containing one or more spectral species in each well of the calibration plate. Each well is then exposed to an excitation source that causes the one or more spectral species in each of the wells to fluoresce. The signal response is measured and associated with each spectral species at each different well position in the calibration plate. Next, the measured signal response from each spectral species at each well position in the calibration plate is compared with a predetermined signal response for each spectral species. The results of this comparison can be used to determine a calibration factor for each well and spectral species to compensate for the difference between the measured signal response and the predetermined signal response.
Abstract translation: 本发明的实施方式描述了一种用于产生光谱检测器仪器的校准因子的装置。 校准因子来源于校准板的每个孔中含有一个或多个光谱种类的校准板。 然后将每个孔暴露于激发源，使得每个孔中的一个或多个光谱物质发荧光。 测量信号响应并与校准板中每个不同孔位置处的每个光谱种类相关联。 接下来，将来自校准板中每个孔位置处的每个光谱种类的测量信号响应与每个光谱种类的预定信号响应进行比较。 该比较的结果可用于确定每个孔和光谱种类的校准因子，以补偿测量的信号响应与预定信号响应之间的差异。
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16.发明申请公开(公告)号：US20080308160A1
公开(公告)日：2008-12-18
申请号：US12221604
申请日：2008-08-05
Applicant: Steven J. Boege , Mark F. Oldham , Marc Haberstroh , Stephen J. Gunstream , Adrian Fawcett , Douglas W. Grunewald
Inventor： Steven J. Boege , Mark F. Oldham , Marc Haberstroh , Stephen J. Gunstream , Adrian Fawcett , Douglas W. Grunewald
IPC: F16L53/00
CPC classification number: H05B6/108 , B01F13/0818 , B01L3/50851 , B01L2300/0803 , B01L2300/0809 , B01L2300/1816 , H05B6/109 , H05B2214/04 , Y10T137/6606
Abstract: A device is provided that comprises one or more fluid retainment regions each having at least one wall, and one or more loops in heat-transfer communication with the at least one wall. Each of the loops can comprise an electrical conductor that surrounds the same or a different fluid retainment region. A device is provided that comprises one or more fluid retainment regions each having particulates disposed therein. A system is provided that includes a platen adapted to hold a device including fluid retainment regions and one or more electrical conductors in heat-transfer communication with the fluid retainment regions. Methods of heating a sample are also provided.
Abstract translation: 提供了一种装置，其包括一个或多个流体保持区域，每个流体保持区域各自具有至少一个壁，以及与所述至少一个壁热传递连通的一个或多个环路。 每个环可以包括围绕相同或不同流体保持区域的电导体。 提供了一种装置，其包括一个或多个流体保持区域，每个流体保持区域各自具有设置在其中的微粒。 提供了一种系统，其包括适于保持包括流体保持区域的装置和与流体保持区域传热连通的一个或多个电导体的压板。 还提供了加热样品的方法。
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公开(公告)号：US20080209978A1
公开(公告)日：2008-09-04
申请号：US12022098
申请日：2008-01-29
Applicant: Stephen J. Gunstream
Inventor： Stephen J. Gunstream
IPC: G12B13/00
CPC classification number: C12Q1/6851 , G01N21/274 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2021/6441 , G01N2201/12 , G01N2201/12746 , G16B30/00 , C12Q2545/113 , C12Q2545/101
Abstract: Methods for generating a calibration factor and for calibrating are provided whereby interstitial spacing between support locations of a platform is used to embed a detectable material, emissions from which are used to generate the calibration factor. In some embodiments, the external space, outer perimeter, and other areas of a platform are used to embed detection materials for the normalization, calibration, correction, compensation, or other method of adjustment for detected emission data. The emission data can be taken from an assay, amplification, reaction, analysis, or other process, for example, from a PCR run or other reaction. By calibrating or adjusting the sample support with the detected emission data, a more efficient detection of the assay, amplification, reaction, analysis, or other process, can be achieved.
Abstract translation: 提供用于产生校准因子和校准的方法，由此使用平台的支撑位置之间的间隙来嵌入可检测材料，用于产生校准因子的发射。 在一些实施例中，平台的外部空间，外部周边和其他区域用于嵌入检测材料，用于检测发射数据的归一化，校准，校正，补偿或其他调整方法。 发射数据可以从测定，扩增，反应，分析或其它过程中获得，例如，从PCR运行或其他反应。 通过使用检测到的排放数据校准或调整样品支架，可以实现更有效的检测，扩增，反应，分析或其他过程。
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18.发明申请公开(公告)号：US20080182264A1
公开(公告)日：2008-07-31
申请号：US12022087
申请日：2008-01-29
Applicant: Stephen J. Gunstream
Inventor： Stephen J. Gunstream
CPC classification number: C12Q1/6851 , G01N21/274 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2021/6441 , G01N2201/12 , G01N2201/12746 , G06F19/22 , C12Q2545/113 , C12Q2545/101
Abstract: Systems and methods for normalizing detected emission data collected in real-time polymerase chain reaction (RT-PCR) and other reactions, are provided. In some embodiments, a sample plate can be loaded with a fluorescent dye and subjected to a real-time PCR reaction. During the initial cycles, detected emissions that correspond to the background signal contributed by the plate, buffer, and other non-reactant pieces of the reaction system and chemistry can be identified. The raw emission data can be normalized by dividing the emission data by the identified baseline signal. According to various embodiments, the normalized amplification profile can normalize to an initial value of 1, because the actual signal emerges from the baseline at the point exponential growth begins. A normalized amplification profile based on a ratio to the baseline can create a more uniformly scaled amplification curve across different samples, filters, wells, dyes, or machines.
Abstract translation: 提供了在实时聚合酶链反应（RT-PCR）和其他反应中收集的检测到的排放数据归一化的系统和方法。 在一些实施方案中，样品板可以装载荧光染料并进行实时PCR反应。 在初始循环期间，可以鉴定出与反应体系和化学物质的板，缓冲液和其它非反应物片段所贡献的背景信号相对应的检测到的排放物。 可以通过将发射数据除以所识别的基线信号来对原始发射数据进行归一化。 根据各种实施例，归一化放大曲线可以归一化为初始值1，因为实际信号在指数增长点开始从基线出现。 基于与基线的比率的归一化扩增曲线可以在不同样品，滤器，孔，染料或机器上产生更均匀的缩放扩增曲线。
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