Minimizing effects of dye crosstalk
    1.
    发明授权
    Minimizing effects of dye crosstalk 有权
    减少染料串扰的影响

    公开(公告)号:US07839507B2

    公开(公告)日:2010-11-23

    申请号:US11770443

    申请日:2007-06-28

    CPC classification number: G01N21/6428 G01N21/274 G01N2021/6421

    Abstract: Methods and systems for processing signals to minimize the effects of dye crosstalk. Deconvolution of multiplexed dye signals can include corrections for residual error determined from experimental measurements. Residual error corrections can account for reaction or assay specific factors and modify the subsequent filtering of signals. Correction values can be determined for specific dye-probe conjugates to minimize dye crosstalk and may be combined with residual error correction to further minimize errors in spectral deconvolution. Apparatus, systems, and computer-readable media can process signals and modify filters based on values obtained using the methods.

    Abstract translation: 用于处理信号的方法和系统,以最小化染料串扰的影响。 多重染料信号的去卷积可以包括由实验测量确定的残差的校正。 残留误差校正可以解释反应或测定特异性因素,并修改随后的信号过滤。 可以针对特定的染料 - 探针缀合物确定校正值以最小化染料串扰,并可与残留误差校正组合以进一步最小化光谱去卷积中的误差。 装置,系统和计算机可读介质可以基于使用这些方法获得的值处理信号和修改滤波器。

    Methods for monitoring polymerase chain reactions
    3.
    发明授权
    Methods for monitoring polymerase chain reactions 有权
    监测聚合酶链反应的方法

    公开(公告)号:US07875425B2

    公开(公告)日:2011-01-25

    申请号:US11270841

    申请日:2005-11-09

    Abstract: A method for calibrating temperature can include cycling temperatures of a set of wells, wherein each well of the set comprises a sample with a spectrally distinguishable species. The method can further include measuring a signal from the spectrally distinguishable species for each well at a temperature during a first temperature cycle, and calibrating the temperatures for measuring the signal from each well during subsequent temperature cycles.

    Abstract translation: 用于校准温度的方法可以包括一组孔的循环温度,其中该组的每个孔包含具有光谱可区分物种的样品。 该方法还可以包括在第一温度循环期间的温度下测量来自每个孔的光谱可区分物质的信号,并且在随后的温度循环期间校准用于测量来自每个孔的信号的温度。

    Systems and methods for baseline correction using non-linear normalization
    5.
    发明授权
    Systems and methods for baseline correction using non-linear normalization 有权
    使用非线性归一化进行基线校正的系统和方法

    公开(公告)号:US08005628B2

    公开(公告)日:2011-08-23

    申请号:US12022087

    申请日:2008-01-29

    Abstract: Systems and methods for normalizing detected emission data collected in real-time polymerase chain reaction (RT-PCR) and other reactions, are provided. In some embodiments, a sample plate can be loaded with a fluorescent dye and subjected to a real-time PCR reaction. During the initial cycles, detected emissions that correspond to the background signal contributed by the plate, buffer, and other non-reactant pieces of the reaction system and chemistry can be identified. The raw emission data can be normalized by dividing the emission data by the identified baseline signal. According to various embodiments, the normalized amplification profile can normalize to an initial value of 1, because the actual signal emerges from the baseline at the point exponential growth begins. A normalized amplification profile based on a ratio to the baseline can create a more uniformly scaled amplification curve across different samples, filters, wells, dyes, or machines.

    Abstract translation: 提供了在实时聚合酶链反应(RT-PCR)和其他反应中收集的检测到的排放数据归一化的系统和方法。 在一些实施方案中,样品板可以装载荧光染料并进行实时PCR反应。 在初始循环期间,可以鉴定出与反应体系和化学物质的板,缓冲液和其它非反应物片段所贡献的背景信号相对应的检测到的排放物。 可以通过将发射数据除以所识别的基线信号来对原始发射数据进行归一化。 根据各种实施例,归一化放大曲线可以归一化为初始值1,因为实际信号在指数增长点开始从基线出现。 基于与基线的比率的归一化扩增曲线可以在不同样品,滤器,孔,染料或机器上产生更均匀的缩放扩增曲线。

    SYSTEMS AND METHODS FOR BASELINE CORRECTION USING NON-LINEAR NORMALIZATION
    6.
    发明申请
    SYSTEMS AND METHODS FOR BASELINE CORRECTION USING NON-LINEAR NORMALIZATION 审中-公开
    使用非线性正规化进行基线校正的系统和方法

    公开(公告)号:US20130041595A1

    公开(公告)日:2013-02-14

    申请号:US13584950

    申请日:2012-08-14

    Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

    Abstract translation: 提供了系统和方法,用于校准在聚合酶链式反应(PCR),扩增反应,测定,过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔,容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据,可以获得更准确的发射数据或信息信号读数。

    System and method for interpolative calibration
    7.
    发明授权
    System and method for interpolative calibration 有权
    内插校准系统和方法

    公开(公告)号:US08285492B2

    公开(公告)日:2012-10-09

    申请号:US12534742

    申请日:2009-08-03

    Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

    Abstract translation: 提供了系统和方法,用于校准在聚合酶链式反应(PCR),扩增反应,测定,过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔,容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据,可以获得更准确的发射数据或信息信号读数。

    Systems And Methods For Baseline Correction Using Non-Linear Normalization
    8.
    发明申请
    Systems And Methods For Baseline Correction Using Non-Linear Normalization 有权
    使用非线性规范化进行基线校正的系统和方法

    公开(公告)号:US20110231132A1

    公开(公告)日:2011-09-22

    申请号:US13070273

    申请日:2011-03-23

    Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

    Abstract translation: 提供了系统和方法,用于校准在聚合酶链式反应(PCR),扩增反应,测定,过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔,容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据,可以获得更准确的发射数据或信息信号读数。

    System and Method for Interpolative Calibration
    9.
    发明申请
    System and Method for Interpolative Calibration 有权
    系统与方法的插值校准

    公开(公告)号:US20100198525A1

    公开(公告)日:2010-08-05

    申请号:US12534742

    申请日:2009-08-03

    Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

    Abstract translation: 提供了系统和方法,用于校准在聚合酶链式反应(PCR),扩增反应,测定,过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔,容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据,可以获得更准确的发射数据或信息信号读数。

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